Hitachi OPTIGEN AP 720STM User Manual

AP 720STM  
LCD Panel Guide  
OPTIGEN®  
Semi-Automated Instrument  
Table of Contents  
Page  
1
Operation Key…………………………………………………………...  
Screen Display and Operation Method  
3
2
2-1  
2-2  
2-3  
2-4  
2-5  
2-6  
2-7  
2-8  
2-9  
Initialization………………………………………………………  
Main Menu……………………………………………………….  
Panel Type Selection……………………………………………..  
Auto-Run (Process 1 to 7)……………………………………….  
Auto-Run (Photoreagent Setup and Process 8)………………….  
Auto-Run (Process End)…………………………………………  
Maintenance……………………………………………………...  
Pause……………………………………………………………..  
Start/End Process and Position Setup…………………………….  
4
5
6
7
9
10  
11  
13  
16  
17  
2-10 Alarm……………………………………………………………..  
3
Flowchart………………………………………………………………...  
21  
2
1 Operation Key  
Display:  
Liquid Crystal Panel  
Operation Key:  
Only the touch panel key in the shaded field is executable. Press the Operation Key  
to execute the function. See Figure 1.  
Numerical Value Input Key:  
Numerical value can be registered by ‘ENT’ key. If the value entered is out of the  
defined range, beep sounds will be on for 3 times. ‘ESC’ key is used for returning  
to the original value. See Figure 2.  
(Figure 1)  
(Figure 2)  
3
2 Screen Display and Operation Method  
2-1 Initialization  
[Screen Display]  
(Figure 3)  
(Figure 4)  
[Summary]  
Figure 3 will display approximately 5 seconds after the power is turned on. Then  
the screen will change automatically to the initialization screen, Figure 4. After the  
completion of initialization, there is a beep sound and Main Menu screen, Figure 5  
will display.  
[Operation Key]  
(Figure 4)  
Pause:  
To Figure 27 Pause screen  
4
2 Screen Display and Operation Method  
2-2 Main Menu  
[Screen Display]  
(Figure 5)  
(Figure 6)  
[Summary]  
Figure 5 is the Main Menu screen after completion of Figure 4. Panel 36 is the  
default panel.  
Figure 6 is the Main Menu screen after selecting Panel 20 in Figure 7.  
[Operation Key]  
Panel 36:  
To Figure 7 Panel Type Selection screen  
To Figure 8 Auto-Run screen  
Next:  
Maintenance:  
To Figure 19 Maintenance screen  
5
2 Screen Display and Operation Method  
2-3 Panel Type Selection  
[Screen Display]  
(Figure 7)  
[Summary]  
Select 36-allergen or 20-allergen panel in Figure 7.  
[Operation Key]  
36:  
To Figure 5 Main Menu screen  
20:  
To Figure 6 Main Menu screen  
6
2 Screen Display and Operation Method  
2-4 Auto-Run (Process 1 to Process 7)  
[Screen Display]  
(Figure 8)  
(Figure 9)  
(Figure 10)  
(Figure 12)  
(Figure 11)  
(Figure 13)  
(Figure 14)  
(Continued on next page)  
7
(Auto-Run, cont.)  
[Summary]  
Figure 8 is a confirmation screen. The Operator needs to check that the Wash  
Buffer Bottle and Antibody Reservoir are filled, and that the Waste Bottle is  
emptied prior to the Auto-Run. Press “Start” key. The following screens will be  
displayed in order and Auto-Run will be executed:  
o Figure 9 displays the priming is in process.  
o Figure 10 displays the Rack and Pette setup is being checked.  
o Figure 11 displays the Rack and Pette setup is confirmed.  
o Figure 12 displays the washing process (Process 1, 4 or 7) or aspiration  
process (Process 2 or 5) is in operation. See Chapter 6, Operation, in the AP  
720S Instruction Manual.  
o Figure 13 shows incubation process (Process 3 or 6) is in operation.  
o Figure 14 is a warning screen during incubation. It indicates the door is open  
with a beep sound. After the door is closed, the screen will return to Figure 13.  
In the case the door is open during the operation, i.e. initialization, priming,  
rack & pettes check, washing, aspirating, rinsing and draining, the screen will  
switch to Pause (see section 2-8 Pause)  
[Operation Key]  
(Figure 8)  
Start: To Figure 9 Priming screen  
Back: To Figure 5 Main Menu screen  
(Figure 9)  
Pause: To Figure 28 Pause screen  
(Figure 10)  
Pause: To: Figure 29 Pause screen  
(Figure 11)  
Pause: To: Figure 29 Pause screen  
(Figure 12)  
Pause: To: Figure 30 Pause screen  
8
2 Screen Display and Operation Method  
2-5 Auto-Run (Photoreagent Setup and Process 8)  
[Screen Display]  
(Figure 15)  
(Figure 16)  
(Figure 17)  
[Summary]  
Figure 15 will alarm Operator that Photoreagent is needed. The display shows the  
elapsed time from the start of auto-pause. “Alarm Off” key will stop the alarm.  
“Continue” key will shift the screen to Figure 16.  
Figure 16 requests confirmation of Photoreagent setup. “No” key will return to the  
previous screen. “Yes” key will shift the screen to Figure 17 and continue  
Auto-Run. Please note that before pressing “Yes” key, the Operator needs to fill the  
Photoreagent Reservoir with Photoreagent mixture and load into Sample/Reagent  
Rack. See instructions in AP 720S Instruction Manual.  
[Operation Key]  
(Figure 15)  
Alarm Off:  
Continue:  
Stop beep sound  
To Figure 16 screen  
(Figure 16)  
Yes:  
No:  
To Figure 17 screen  
To Figure 15 screen  
(Figure 17)  
Pause:  
To Figure 30 Pause screen  
9
2 Screen Display and Operation Method  
2-6 Auto-Run (Process End)  
[Screen Display]  
(Figure 18)  
[Summary]  
Figure 18 will alarm the Operator of the completion of Auto-Run. The display  
shows the elapsed time from the completion of Auto-Run. “Alarm Off” key will  
stop the alarm. “End” key will return to the Main Menu screen, Figure 5. “Rinse”  
key will shift the screen to Figure 22.  
[Operation Key]  
(Figure 18)  
Alarm Off:  
End:  
Rinse:  
Stop beep sound  
To Figure 5 Main Menu screen  
To Figure 22 screen  
10  
2 Screen Display and Operation Method  
2-7 Maintenance  
[Screen Display]  
(Figure 19)  
(Figure 20)  
(Figure 22)  
(Figure 24)  
(Figure 21)  
(Figure 23)  
(Figure 25)  
(Figure 26)  
(Continued on next page)  
11  
(Maintenance, cont.)  
[Summary]  
Figure 19 provides the Prime, Rinse and Clock functions.  
[Operation Key]  
(Figure 19)  
Prime: To Figure 20 Prime Operation screen  
Rinse: To Figure 22 Rinse Operation screen  
Clock: To Figure 26 Time Setup screen  
Back: To Figure 5 Main Menu screen  
(Figure 20)  
Prior to Prime Operation, the Operator needs to set the Wash Buffer tube into  
the Wash Buffer Bottle, the water tube into the Deionized Water Bottle, &  
empty the Waste Bottle. “Ok” key will start priming operation.  
Ok: To Figure 21 Priming Operation screen  
Back: To Figure 19 Maintenance screen  
(Figure 22)  
Prior to Rinse Operation, the Operator needs to set the Wash Buffer tube and  
water tube into Deionized Water Bottle. “Ok” key will start rinsing operation.  
Ok: To Figure 23 Rinse Operation screen  
Back: To Figure 19 Maintenance screen  
(Figure 24)  
Prior to Drain Operation, the Operator needs to pull out the tubes from Wash  
Buffer Bottle and Deionized Water Bottle. “Ok” key will start draining  
operation.  
Ok: To Figure 25 Drain Operation screen  
End: To Figure 19 Maintenance screen  
(Figure 26)  
Internal clock of the machine can be set in this screen.  
Year: Figure 2 numerical value input screen with title [#Years]  
Month: Figure 2 numerical value input screen with title [#Months]  
Day: Figure 2 numerical value input screen with title [#Days]  
Hour: Figure 2 numerical value input screen with title [#Hours]  
Minute: Figure 2 numerical value input screen with title [#Minutes]  
Second: Figure 2 numerical value input screen with title [#Seconds]  
Set: Clock is set, return to Figure 19 Maintenance screen  
Back: Clock is not set, return to Figure 19 Maintenance screen  
12  
2 Screen Display and Operation Method  
2-8 Pause  
[Screen Display]  
(Figure 27)  
(Figure 28)  
(Figure 30)  
(Figure 32)  
(Figure 29)  
(Figure 31)  
(Figure 33)  
(Figure 34)  
(Continued on next page)  
13  
(Pause, cont.)  
[Summary]  
Figure 27 to Figure 33 the alarm will indicate that the current operation is paused.  
The screen will display the [Run Status] including the time elapsed from the start  
of the pause. A message “Information=” may be displayed in the screen, Figure 33.  
See [Supplement] below for details.  
“Continue” key will shift the screen to the original status, and resume the operation.  
For example, in Figure 30 Process was paused at pette #12 and the operation will  
resume on pette #12. “Cancel” key will shift the screen to Figure 34. “No” key  
will return to the previous screen. Yes” key will forward to Figure 3.  
[Operation Key]  
(Figure 27) to (Figure 33)  
Alarm Off:  
Continue:  
Cancel:  
Stop beep sound  
Return to the original screen and operation is resumed  
To Figure 34  
(Figure 34)  
Yes:  
No:  
To Figure 3  
To previous screen  
[Supplement]  
At the time of operation failure, the instrument will automatically pause and  
various warning message will be displayed on the screen. Even though operation  
can resume, if causes of failure remain, the instrument will pause again. Please call  
Service Representative for additional assistance.  
The following are examples of alarm messages:  
o Example) “Information=D. Open”  
“D. Open” indicates that the door is open after the start of run operation. Press  
“CONTINUE” key after the door is closed.  
o Example) “Information =C1 L”  
“C1 L” indicates failure from both sides of head part of carrier drive or sensor  
failure. After you confirm the status, press “CONTINUE” key and check the  
status.  
o Example) “Information =C1 H”  
“C1 H” indicates failure from both sides of head part of carrier drive or sensor  
failure. After you confirm the status, press “CONTINUE” key and check the  
status.  
(Continued on next page)  
14  
(Examples of alarm messages, cont.)  
o Example) “Information =C2 L”  
“C2 L” indicates failure from the head part of top/bottom carrier drive or sensor  
failure. After you confirm the status, press “CONTINUE” key and check the  
status.  
o
Example) “Information =C2 H”  
“C2 H” indicates failure from the head part of top/bottom carrier drive or  
sensor failure. After you confirm the status, press “CONTINUE” key and check  
the status.  
o Example) “Information =C3 L”  
“C3 L” indicates failure of PET rack rotation carrier drive or sensor failure.  
After you confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C3 H”  
“C3 H” indicates failure of PET rack rotation carrier drive or sensor failure.  
After you confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C4 L”  
“C4 L” indicates failure of reagent rack rotation carrier drive or sensor failure.  
After you confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C4 H”  
“C4 H” indicates failure of reagent rack rotation carrier drive or sensor failure.  
After you confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C5 L”  
“C5 L” indicates failure of aspiration syringe drive or sensor failure. After you  
confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C5 H”  
“C5 H” indicates failure of aspiration syringe drive or sensor failure. After you  
confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C6 L”  
“C6 L” indicates failure of wash syringe drive or sensor failure. After you  
confirm the status, press “CONTINUE” key and check the status.  
o Example) “Information =C6 H”  
“C6 H” indicates failure of wash syringe drive or sensor failure. After you  
confirm the status, press “CONTINUE” key and check the status.  
15  
2 Screen Display and Operation Method  
2-9 Start/End Process and Position Setup  
[Screen Display]  
(Figure 35)  
Start  
End  
Process  
Process  
[Summary]  
Figure 35 will appear when power interruption occurs during Auto-Run or the  
current process is cancelled/paused during Auto-Run.  
Before re-starting the Auto-Run, the Operator needs to setup the START  
PROCESS NO., END PROCESS NO. and PETTE POSITION NO. according to  
the status at the interruption of Auto-Run so that the operation will be continued  
without missing steps.  
Start Process:  
End Process:  
Position:  
Range 1-8 (a smaller number than End Process)  
Range 1-8 (a greater number than Start Process)  
Pette start position, range 1-20  
[Operation Key]  
(Figure 35)  
Start Process key:  
To Figure 2 numerical value input screen with the  
title [Start Process]  
End Process key:  
To Figure 2 numerical value input screen with the  
title [End Process]  
Start Position key:  
To Figure 2 numerical value input screen with the  
title [Start Position]  
Next:  
Back:  
To Figure 8 Auto-Run screen  
To Figure 5 Main Menu screen  
16  
2 Screen Display and Operation Method  
2-10 Alarm  
[Summary]  
The alarm screen will appear when a problem occurs during the Auto-Run. The  
alarm will sound and can be stopped by pressing the “Alarm Off” key.  
There are two kinds of alarm screens. The first alarm screen is for confirmation.  
The Operator will confirm the failure or error displayed on the screen. Press “Ok”  
key to proceed to the next process. The second alarm screen requires action. The  
Operator needs to make a proper correction according to the screen display, and  
then make the selection to “Retry” or “End” the process.  
Figure 36 Alarm screen of power interruption (confirmation)  
Figure 37 Alarm screen of data initialization (confirmation)  
Figure 38 Alarm screen of no Sample/Reagent Rack (selection)  
Figure 39 Alarm screen of no pettes (selection)  
Figure 40 Alarm screen of wrong pette setup (selection)  
Figure 41 Alarm screen of wrong start pette setup (selection)  
Figure 36 - Alarm Screen of Power Interruption (Confirmation)  
[Screen Display]  
(Figure 36)  
[Summary]  
If there is a power failure during the Auto-Run, the control software will detect the  
power interruption after the power has returned and Figure 36 will appear. The  
elapsed time from the start of power interruption will be displayed. Press ‘Ok’  
key to continue the process.  
17  
Figure 37 - Alarm Screen of Data Initialization (Confirmation)  
[Screen Display]  
(Figure 37)  
[Summary]  
Figure 37 shows data error has occurred after initialization. The Operator needs to  
press “Ok” so that the data for the instrument will be recovered and the default  
values will be implemented. Re-entry of program and internal parameters may be  
required. If this is the case, please contact your Service Representative.  
Figure 38 - Alarm Screen of No Sample/Reagent Rack  
[Screen Display]  
(Figure 38)  
[Summary]  
If the control software detects that there is no Sample/Reagent Rack at the start of  
the run, Figure 38 will appear. If the run needs to be continued, setup the  
Sample/Reagent Rack, and press ‘Retry’ to continue. If the run needs to be  
cancelled, press ‘End’ key to end the Auto-Run.  
18  
Figure 39 - Alarm Screen of No Pettes  
[Screen Display]  
(Figure 39)  
[Summary]  
If the control software detects that there is no pette at the start of the run, Figure 39  
will appear. If the run needs to be continued, setup pettes in the Pette Rack, and  
press ‘Retry’ key to continue. If the run needs to be cancelled, press ‘End’ key to  
end the Auto-Run.  
Figure 40 - Alarm Screen of Wrong Pette Setup  
[Screen Display]  
(Figure 40)  
[Summary]  
If the control software detects that the pette setup is wrong at the start of the run,  
Figure 40 will appear. If the run needs to be continued, setup pettes correctly and  
press ‘Retry’ key to continue. If the run needs to be cancelled, press ‘End’ key to  
end the Auto-Run.  
Figure 40 shows that the last pette is detected at position #10, and a wrong pette  
setup is detected at position #12. This is because no pette is detected at position  
#11.  
When “End # 0” is shown, pette is not detected at position #1.  
19  
Figure 41 - Alarm Screen of Wrong Start Pette Setup  
[Screen Display]  
(Figure 41)  
[Summary]  
If the control software detects that the position of the start pette is wrong at the  
start of the run, Figure 41 will appear. If the run needs to be continued, setup the  
pettes correctly, press ‘Retry’ key to continue. If the run needs to be cancelled,  
press ‘End’ key to end the Auto-Run.  
Figure 41 shows that the pettes are loaded from position #1 to #4. However, the  
Operator entered position #6 for the pette start position (refer to section 2-9).  
Figure 42 - Alarm Screen of Communication Error  
[Screen Display]  
(Figure 42)  
[Summary]  
After the power is turned on, Figure 42 will appear if the control software cannot  
communicate with the panel operation. The version information and current  
date/time will not be displayed in the screen as Figure 3 shows. The date/time  
display will count up from 80/1/10:0:0.  
When this alarm screen occurs, normal machine function is not possible.  
Please contact your Service Representative.  
20  
Pause  
Auto-Run  
AP720S™ Panel Flowchart  
Maintenance  
Interrupted run  
21  

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