Leica Dollhouse NCL CK17 User Manual

TM  
Novocastra Lyophilized  
Mouse Monoclonal Antibody  
Cytokeratin 17  
Product Code: NCL-CK17  
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.  
Intended Use  
Specificity  
Clone  
Human cytokeratin 17 intermediate filament protein.  
E3  
IgG2b  
Ig Class  
Keratin preparation from rat enterocytes.  
Antigen Used for  
Immunizations  
Mouse myeloma (X63-Ag8.653).  
Hybridoma Partner  
Lyophilized tissue culture supernatant containing sodium azide.  
Reconstitute with the volume of sterile distilled water indicated on the vial label.  
Preparation  
Yes  
Yes  
Effective on Frozen Tissue  
Effective on Paraffin Wax  
Embedded Tissue  
Immunohistochemistry on paraffin sections.  
Recommendations on Use  
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra  
Epitope Retrieval Solution pH6.  
Suggested dilution: 1:20 for 30 minutes at 25 °C. This is provided as a guide and users should  
determine their own optimal working dilutions.  
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.  
For further product information or support, contact your local distributor or regional office of Leica  
The performance of this antibody should be validated when utilized with other manual staining  
systems or automated platforms.  
Immunohistochemistry: Trachea.  
Western Blotting: HeLa cell line.  
Positive Controls  
Cytoplasmic.  
Staining Pattern  
Store unopened antibody at 2–8 °C. Under these conditions, there is no significant loss in product  
performance up to the expiry date indicated on the vial label. Do not use after expiration date  
indicated on the vial label. The reconstituted antibody is stable for at least two months when  
stored at 2–8 °C. For long term storage, it is recommended that aliquots of the reconstituted  
antibody are stored frozen at -20 °C (frost-free freezers are not recommended). Repeated  
freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to 2–8  
°C immediately after use. Storage conditions other than those specified above must be verified  
by the user.  
Storage and Stability  
This reagent has been prepared from the supernatant of cell culture confirm. As it is a biological  
product, reasonable care should be taken when handling it. This reagent contains sodium azide.  
A Material Safety Data Sheet is available upon request or available from  
Warnings and Precautions  
Leica Biosystems Newcastle Ltd  
Balliol Business Park West  
Benton Lane  
Newcastle Upon Tyne NE12 8EW  
United Kingdom  
(
+44 191 215 4242  
© Leica Biosystems Newcastle Ltd NCL- Rev D CK17 21/01/2014  
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