Roper Digital Camera CoolSnap User Guide

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© Copyright 2005  
Roper Scientific, Inc.  
3440 East Britannia Drive  
Tucson, Arizona 85706  
Tel: 800.874.9789/520.889.9933  
Fax: 520.295.0299  
All rights reserved. No part of this publication may be reproduced by any means without the written  
permission of Roper Scientific, Inc.  
Printed in the United States of America.  
CoolSNAP is a trademark of Roper Scientific, Inc.  
Photometrics, PVCAM, and Roper Scientific are registered trademarks of Roper Scientific, Inc.  
Acrobat and Reader are registered trademarks of Adobe Systems Incorporated in the United States and/or  
other countries.  
Mac, Macintosh, and Panther are trademarks of Apple Computer, Inc., registered in the U.S. and other  
countries.  
Pentium is a registered trademark of Intel Corporation. Xeon is a trademark of Intel Corporation.  
Windows is a registered trademark of Microsoft Corporation.  
Other brand and product names are the trademarks or registered trademarks of their respective owners and  
manufacturers.  
The information in this publication is believed to be accurate as of the publication release date. However,  
Roper Scientific does not assume any responsibility for any consequences including any damages resulting  
from the use thereof. The information contained herein is subject to change without notice. Revision of this  
publication may be issued to incorporate such change.  
57-061-001 Rev G0  
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LIMITED WARRANTY — Photometrics Analytical Instrumentation  
Photometrics, a division of Roper Scientific, Inc. (“Photometrics,” us,” “we,” “our”) makes the following  
limited warranties. These limited warranties extend to the original purchaser (“You”, “you”, "Your", "your")  
only and no other purchaser or transferee. We have complete control over all warranties and may alter or  
terminate any or all warranties at any time we deem necessary.  
Basic Limited One (1) Year Warranty  
Photometrics warrants this product against substantial defects in materials and/or workmanship for a period  
of up to one (1) year after shipment. During this period, Photometrics will repair the product or, at its sole  
option, repair or replace any defective part without charge to you. You must deliver the entire product to the  
Photometrics factory or, at our option, to a factory-authorized service center. You are responsible for the  
shipping costs to return the product. International customers should contact their local Photometrics  
authorized representative/distributor for repair information and assistance, or visit our technical support  
page at www.photomet.com.  
Limited One (1) Year Warranty on Refurbished or Discontinued Products  
Photometrics warrants, with the exception of the CCD imaging device (which carries NO WARRANTIES  
EXPRESS OR IMPLIED), this product against defects in materials or workmanship for a period of up to one  
(1) year after shipment. During this period, Photometrics will repair or replace, at its sole option, any defective  
parts, without charge to you. You must deliver the entire product to the Photometrics factory or, at our option,  
a factory-authorized service center. You are responsible for the shipping costs to return the product to  
Photometrics. International customers should contact their local Photometrics representative/distributor for  
repair information and assistance or visit our technical support page at www.photomet.com.  
Normal Wear Item Disclaimer  
Photometrics does not warrant certain items against defect due to normal wear and tear. These items include  
internal and external shutters, cables, and connectors. These items carry no warranty, expressed or implied.  
Shutter Warranty  
Photometrics warrants the standard, factory-installed shutter of all our products that incorporate an  
integrated shutter for a period of twelve (12) months. This warranty applies to the standard shutter installed  
in the camera system at the time of manufacture. Non-standard shutters, SPR (special product request)  
shutters, and third-party shutter drive equipment carry no warranty expressed or implied. Photometrics will  
supply, at no cost to the customer, up to one (1) replacement shutter during the warranty period.  
Photometrics will, at Photometrics option, either ship a ready-to-install shutter to the customer site for  
installation by the customer according to the instructions in the product User Manual or arrange with the  
customer to return the camera system (or portion of the camera system) to the factory (or factory authorized  
service center) for shutter replacement by a factory-authorized agent. Responsibility for transportation and  
insurance charges is described above.  
Vacuum Integrity Limited 24 Month Warranty  
Photometrics warrants the vacuum integrity of all our products for a period of up to twenty-four (24) months  
from the date of shipment. We warrant that the detector head will maintain the factory-set operating  
temperature without the requirement for customer pumping. Should the detector experience a Vacuum  
Integrity failure at anytime within twenty-four (24) months from the date of delivery all parts and labor  
needed to restore the vacuum integrity will be covered by us. Responsibility for shipping charges is as  
described above under our Basic Limited One (1) Year Warranty.  
X-Ray Detector Limited One Year Warranty  
Photometrics warrants, with the exception of CCD imaging device and fiber optic assembly damage due to X-  
rays (which carry NO WARRANTIES EXPRESSED OR IMPLIED), all X-ray products for one (1) year after  
shipment. See additional Basic Limited One (1) year Warranty terms and conditions above, which apply to this  
warranty. Responsibility for shipping charges is as described above under our Basic Limited One (1) Year  
Warranty.  
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Software Limited Warranty  
Photometrics warrants all of our manufactured software discs to be free from substantial defects in materials  
and / or workmanship under normal use for a period of one (1) year from shipment. Photometrics does not  
warrant that the function of the software will meet your requirements or that operation will be uninterrupted  
or error free. You assume responsibility for selecting the software to achieve your intended results and for the  
use and results obtained from the software. In addition, during the one (1) year limited warranty, the original  
purchaser is entitled to receive free version upgrades. Version upgrades supplied free of charge will be in the  
form of a download from the Internet. Those customers who do not have access to the Internet may obtain the  
version upgrades on a CD-ROM from our factory for an incidental shipping and handling charge. See Item 12  
in the following section of this warranty ("Your Responsibility") for more information.  
Owner's Manual and Troubleshooting  
You should read the owner’s manual thoroughly before operating this product. In the unlikely event that you  
should encounter difficulty operating this product, the owner’s manual should be consulted before contacting  
the Photometrics technical support staff or authorized service representative for assistance. If you have  
consulted the owner's manual and the problem still persists, please contact the Photometrics technical support  
staff or our authorized service representative. See Item 12 in the following section of this warranty ("Your  
Responsibility") for more information.  
Your Responsibility  
The above Limited Warranties are subject to the following terms and conditions:  
1. You must retain your bill of sale (invoice) and present it upon request for service and repairs or  
provide other proof of purchase satisfactory to Photometrics.  
2. You must notify the Photometrics factory service center within (30) days after you have taken  
delivery of a product or part that you believe to be defective. With the exception of customers who  
claim a “technical issue” with the operation of the product or part, all invoices must be paid in full in  
accordance with the terms of sale. Failure to pay invoices when due may result in the interruption  
and/or cancellation of your one (1) year limited warranty and/or any other warranty, expressed or  
implied.  
3. All warranty service must be made by the Photometrics factory or, at our option, an authorized  
service center.  
4. Before products or parts can be returned for service you must contact the Photometrics factory and  
receive a return authorization number (RMA). Products or parts returned for service without a return  
authorization evidenced by an RMA will be sent back freight collect.  
5. These warranties are effective only if purchased from the Photometrics factory or one of our  
authorized manufacturer's representatives or distributors.  
6. Unless specified in the original purchase agreement, Photometrics is not responsible for installation,  
setup, or disassembly at the customer’s location.  
7. Warranties extend only to defects in materials or workmanship as limited above and do not extend to  
any product or part which has:  
been lost or discarded by you;  
been damaged as a result of misuse, improper installation, faulty or inadequate maintenance or  
failure to follow instructions furnished by us;  
had serial numbers removed, altered, defaced, or rendered illegible;  
been subjected to improper or unauthorized repair; or  
been damaged due to fire, flood, radiation, or other “acts of God” or other contingencies beyond  
the control of Photometrics.  
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8. After the warranty period has expired, you may contact the Photometrics factory or a Photometrics-  
authorized representative for repair information and/or extended warranty plans.  
9. Physically damaged units or units that have been modified are not acceptable for repair in or out of  
warranty and will be returned as received.  
10. All warranties implied by state law or non-U.S. laws, including the implied warranties of  
merchantability and fitness for a particular purpose, are expressly limited to the duration of the  
limited warranties set forth above. With the exception of any warranties implied by state law or non-  
U.S. laws, as hereby limited, the forgoing warranty is exclusive and in lieu of all other warranties,  
guarantees, agreements, and similar obligations of manufacturer or seller with respect to the repair or  
replacement of any parts. In no event shall Photometrics’ liability exceed the cost of the repair or  
replacement of the defective product or part.  
11. This limited warranty gives you specific legal rights and you may also have other rights that may vary  
from state to state and from country to country. Some states and countries do not allow limitations on  
how long an implied warranty lasts, when an action may be brought, or the exclusion or limitation of  
incidental or consequential damages, so the above provisions may not apply to you.  
12. When contacting us for technical support or service assistance, please refer to the Photometrics factory  
of purchase, contact your authorized Photometrics representative or reseller, or visit our technical  
support page at www.photomet.com.  
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Optional CoolSNAP Camera System Service Contract  
The optional CoolSNAP Camera System Service Contract provides extended coverage for the CoolSNAP  
camera system. All terms and conditions listed in the CoolSNAP Camera System Warranty, including those  
pertaining to shipping costs and appropriate system use, apply to the CoolSNAP Camera System Service  
Contract.  
The Service Contract also guarantees that Photometrics shall ship a complete replacement CoolSNAP camera  
system within forty-eight (48) hours of receiving notification of a CoolSNAP camera system defect. All  
components of the defective CoolSNAP camera system must be returned to Photometrics. Shipment of these  
components should not be made without prior authorization by Photometrics.  
When purchased within two (2) months of the date listed on the packing slip shipped with the original  
equipment, the Service Contract is valid for a period of twenty-seven (27) months from the packing slip date.  
This period comprises the initial CoolSNAP Camera System Warranty period of twelve (12) months, the  
CoolSNAP Camera System Service Contract period of twelve (12) months, and an additional three (3) months  
of free coverage.  
When purchased prior to the lapse of the CoolSNAP Camera System Warranty or an active CoolSNAP  
Camera System Service Contract, the CoolSNAP Camera System Service Contract is valid for twelve (12)  
months beyond the lapse date of the Camera System Warranty or current Service Contract.  
The CoolSNAP Camera System Service Contract can be renewed repeatedly. If the renewal occurs outside of a  
period covered by Warranty or Service Contract, Photometrics may, at its sole discretion, require an inspection  
of the camera system as a condition of consent to place it under Service Contract.  
U. S. Government Restricted Rights  
The software and documentation are provided with Restricted Rights. Use, duplication, or disclosure by the  
Government is subject to restrictions as set forth in subparagraph (c)(1)(ii) of the Rights in Technical Data and  
Computer Software clause at DFARS 252.227-7013 or subparagraphs (c)(1) and (2) of the Commercial  
Computer Software-Restricted Rights at 48 CFR 52.227-19, as applicable. Contractor/manufacturer is  
Photometrics, 3440 East Britannia Drive, Tucson, AZ 85706.  
This license is effective until terminated. It will terminate upon the conditions set forth above or if you fail to  
comply with any term hereof. Upon termination, you agree that the software and accompanying materials,  
and all copies thereof, will be destroyed. This agreement is governed by the laws of the State of Arizona. You  
acknowledge that you have read this agreement, you understand it, you agree to be bound by its terms, and  
that this is the complete and exclusive statement of the agreement between you and Photometrics regarding  
the software.  
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Declaration of Conformity  
Roper Scientific, Inc. declares that the equipment described in this document is a CLASS A DEVICE in  
conformance with the requirements of the European Council Directives, listed below:  
89/336/EEC  
93/68/EEC  
73/23/EEC  
EMC Directive & Amendments  
EMC Directive & Amendments  
Product Safety with Amendments  
on the application of the laws of Member States relating to Electromagnetic Compatibility and  
Product Safety.  
This declaration is based upon compliance of the product to the following standards:  
EN 55022  
EN 61000-3 Series  
EN 55024  
RF Emissions Control  
Harmonics & Flicker  
Immunity from Electromagnetic Disturbances  
Product Safety  
EN 60950  
Product Description:  
Model:  
Video Camera  
CoolSNAPcf / CoolSNAPES  
Test Reports:  
EMC Rpt 2K01-0122-003B- EN55022  
EMC Rpt 2K00-0122-003C- EN55024  
EMC Rpt 2K01-0122-003D-EN61000-3 Series  
SAFETY Rpt 2K01-0122-003E-EN60950  
Roper Scientific, Inc.  
3440 East Britannica Drive  
Tucson, AZ, 85706  
USA  
Manufacturer:  
ROPER SCIENTIFIC  
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Declaration of Conformity  
Roper Scientific, Inc. declares that the equipment described in this document is a CLASS A DEVICE in  
conformance with the requirements of the European Council Directives, listed below:  
89/336/EEC  
92/31/EEC  
93/68/EEC  
73/23/EEC  
EMC Directive  
Amendment  
Amendment  
Product Safety  
on the application of the laws of Member States relating to Electromagnetic Compatibility and  
Product Safety.  
This declaration is based upon compliance of the product to the following standards:  
EN50082-1, EN60000-3-2  
EN50082-1, EN60000-3-3  
EN50082-1, EN61000-4-2  
EN50082-1, EN61000-4-3  
EN50082-1, EN61000-4-4  
EN50082-1, EN61000-4-5  
EN50082-1, EN61000-4-6  
EN50082-1, EN61000-4-11  
EN55022-1998  
Current Harmonics  
Voltage Fluctuations and Flicker  
Electrostatic Discharge (ESD)  
Radiated Electromagnetic Field (EMF)  
Electrical Fast Transient/Burst (EFT)  
Surge Immunity (Mains)  
RF Injected Current  
Voltage Dips and Interruptions  
with Amendment A1:2000 (CISPR-22)  
Product Description:  
Video Camera  
CoolSNAPcf / CoolSNAPES, IEEE-1394  
Model:  
Authorized Signature:  
E. J. Pisa, Vice President  
Roper Scientific, Inc.  
3440 East Britannica Drive  
Tucson, AZ 85706  
USA  
Manufacturer:  
ROPER SCIENTIFIC  
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Table of Contents  
Chapter 1. Introduction  
Description.................................................................................................................................1  
System Components.................................................................................................................1  
About This Manual...................................................................................................................2  
Precautions.................................................................................................................................2  
Environmental Requirements .................................................................................................2  
Storage Requirements...............................................................................................................3  
Microscopes, Lenses, and Tripods..........................................................................................3  
Repairs........................................................................................................................................3  
Cleaning .....................................................................................................................................3  
Photometrics Customer Service ..............................................................................................4  
Chapter 2. System Installation  
Introduction...............................................................................................................................5  
Software Compatibility Requirements...................................................................................5  
Host Computer Requirements ................................................................................................5  
Multiple Cameras......................................................................................................................6  
Software Installation.................................................................................................................6  
Installing the Interface Card....................................................................................................6  
Connecting Your CoolSNAP Camera ....................................................................................7  
Chapter 3. Operating Features  
Common Features.....................................................................................................................9  
Readout Speed....................................................................................................................9  
Binning ................................................................................................................................9  
CoolSNAPES Features ...............................................................................................................9  
Dual Mode Operation .......................................................................................................9  
Antiblooming ...................................................................................................................10  
Application Examples .....................................................................................................11  
Chapter 4. Troubleshooting  
System Does Not Boot Normally..........................................................................................12  
New Hardware Found Dialog Box Does Not Appear (Windows 2000/XP).......................12  
Green LED Does Not Illuminate (Camera Has No Power)...............................................12  
Images Not Displayed Properly............................................................................................13  
Bright Spots in Image / Increased Background Noise ..........................................................13  
Camera Running Too Warm .................................................................................................13  
PVCAM Error Message Appears..........................................................................................13  
Lengthy Pauses During Imaging ..........................................................................................13  
IEEE-1394 CoolSNAP Camera will Not Image When Attached to Certain Dual-  
processor or Hyperthreading-capable PCs..........................................................................14  
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Chapter 5. Basic Specifications  
Dimensions ..............................................................................................................................15  
CCD Specifications and Orientations...................................................................................17  
Additional Measurements .....................................................................................................17  
Detector ....................................................................................................................................18  
Auxiliary Power Supply.........................................................................................................19  
Usage Requirements........................................................................................................19  
Installation ........................................................................................................................19  
Specifications....................................................................................................................19  
Index........................................................................................................20  
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Chapter 1.  
Introduction  
CoolSNAPcf and CoolSNAPES, from Photometrics®, are ideal cameras for digital  
microscopy and many other biological applications. The simple, compact  
CoolSNAPcf incorporates a high-quality CCD (charge-coupled device), a 12-bit  
scientific digitizer, and low-noise electronics to produce high-quality 12-bit  
monochrome or 36-bit digital color images at greater than 1k x 1k resolution. The  
CoolSNAPES offers higher sensitivity (especially in the red region) and lower read  
noise to produce high quality 12-bit monochrome images.  
Description  
Note: Unless otherwise noted, the CoolSNAPcf and the CoolSNAPES cameras are referred  
to by the name "CoolSNAP".  
Components for the CoolSNAPcamera system are listed as follows:  
System  
Components  
Camera  
Interface hardware (high-speed digital LVDS or IEEE-1394 data-transfer  
interface card)  
Data cable (connects camera and interface hardware)  
Software discs (PVCAM® drivers included)  
CoolSNAP Camera with Test Lens  
CoolSNAP LVDS Cable  
CoolSNAP IEEE-1394 Cable  
CoolSNAP LVDS Interface Card  
CoolSNAP IEEE-1394 Interface Card  
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The CoolSNAP User Manual is divided into five chapters. It is suggested that you  
read the entire manual before operating the camera in order to ensure proper  
use. The chapters that follow this introduction are:  
About This  
Manual  
System Installation — Instructions for installing the camera system’s  
hardware and software.  
Operating Features — Descriptions of specific camera features.  
Troubleshooting — Answers to camera system problems.  
Basic Specifications — Specifications for CoolSNAP components.  
The CCD and other system electronics are extremely sensitive to electrostatic  
discharge (ESD). To avoid permanently damaging the system, please observe  
the following precautions:  
Precautions  
This is a Class A product. In a domestic environment, this product may  
cause radio interference, in which case, the user may be required to take  
adequate measures.  
If you are using high-voltage equipment (such as an arc lamp) with your  
camera system, be sure to turn the camera power on last and power the  
camera off first.  
Never connect or disconnect any cable while the camera system is  
powered on (LVDS cameras only).  
Although you should switch off the camera’s power before disconnecting  
the CoolSNAP cable from the DATA connector or the PCI card, you do  
not need to power off your computer to detach the cable.  
Use caution when triggering high-current switching devices (such as an  
arc lamp) near your system. The CCD can be permanently damaged by  
transient voltage spikes. If electrically noisy devices are present, an  
isolated, conditioned power line or dedicated isolation transformer is  
highly recommended.  
Always leave 1/2 inch of space around the camera’s external cooling  
fins for air flow.  
Never open the camera. There are no user-serviceable parts inside the  
CoolSNAP camera. Opening the camera voids the warranty.  
Use only a CoolSNAP data cable and a CoolSNAP interface card with  
your CoolSNAP camera. Using a different cable or interface card may  
result in permanent damage to your system.  
Do not use a screw longer than 1/4 inch (6.3 mm) in the camera’s tripod  
mounting hole.  
Do not use a C-mount lens having optics that extend farther than .47  
inches (11.9 mm) behind the flange of the lens.  
The CoolSNAP camera system should be operated in a clean, non-condensing  
environment.  
Environmental  
Requirements  
The camera system’s ambient operating temperature is 15°C to 30°C.  
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CoolSNAPcf /CoolSNAPES  
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Store the CoolSNAP camera system in its original containers. To protect the  
system from excessive heat, cold, and moisture, store at an ambient temperature  
between 0°C and 70°C with a relative humidity of 0%-80% noncondensing.  
Storage  
Requirements  
The camera has a standard threaded video mount and can be mounted to any  
microscope that accepts a standard C-mount adapter. The camera also allows  
you to install any lens that is compatible with a standard threaded video mount  
as long as its optics do not extend farther than .47 inches (11.9 mm) behind the  
flange of the lens. The CoolSNAP camera can be mounted to a tripod using the  
tripod-mounting hole located on the bottom of the camera. See Additional  
Measurements on page 17 for more information.  
Microscopes,  
Lenses, and  
Tripods  
Note: In microscopy applications, a 0.5x C-mount camera coupler is recommended for an  
expanded field of view.  
The CoolSNAP camera system contains no user-serviceable parts. Repairs must  
be done by Photometrics. Should your camera system need repair, contact  
Photometrics Customer Service. Please save the original packing materials so  
you can safely ship the camera system to another location or return it for repairs  
if necessary.  
Repairs  
Note: Do not open the camera. Opening the CoolSNAP camera voids the warranty.  
Clean exterior surfaces of the camera with a dry, lint-free cloth. To remove  
stains, contact Photometrics Customer Service.  
Cleaning  
To clean the camera’s imaging window, use only a filtered compressed-air  
source. Do not touch the window.  
Chapter 1. Introduction  
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If you have any questions about your camera system, contact Photometrics  
Customer Service. When you call, please have your Photometrics sales order  
number or equipment serial numbers available.  
Photometrics  
Customer Service  
Tel:  
Fax:  
800. 874.9789/520.889.9933 between 8:00 am and 5:00 pm MST  
520.295.0299  
Mail:  
Photometrics  
3440 East Britannia Drive  
Tucson, Arizona 85706  
In Europe, you can reach Customer Service at:  
BENELUX  
Tel:  
Fax:  
31.347.324989  
31.347.324979  
Mail:  
Roper Scientific, BV  
Ir. D.S. Tuijnmanweg 10  
4131 PN VIANEN, Netherlands  
FRANCE  
Tel:  
Fax:  
33.160.86.03.65  
33.160.86.07.09  
Mail:  
Roper Scientific, SARL  
Z.I. Petite Montagne Sud  
4, rue de l'Oisans - C.E. 1702  
91017 Evry Cedex, France  
GERMANY  
Tel:  
Fax:  
49.89.660.779.3  
49.89.660.779.50  
Mail:  
Roper Scientific, GmbH  
Rosenheimer Landstr. 87  
D-85521 Ottobrunn, Germany  
In Japan, you can reach Customer Service at:  
Tel:  
Fax:  
81.3.5639.2731  
81.3.5639.2775  
Mail:  
Nippon Roper, K.K.  
Sakurai Building  
2-8-19 Fukagawa  
Koto-ku, Tokyo  
Japan 135-0033  
General product information and answers to some customer service questions can  
be found on our website: http://www.photomet.com  
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CoolSNAPcf /CoolSNAPES  
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Chapter 2.  
System Installation  
Carefully review the Precautions section on page 2 before performing any of the  
procedures outlined here. Again, use only a CoolSNAP cable and a CoolSNAP interface  
card with your CoolSNAP camera. Using a different cable or interface card may result in  
permanent damage to your system.  
Your CoolSNAPcf or CoolSNAPES camera system has the following hardware  
components:  
Introduction  
Camera  
Data cable  
Interface card  
CoolSNAP system components are linked by the data cable and controlled by  
your host computer system. All of these hardware components should be  
included with your shipment. Refer to the information and figures in System  
Components on page 1.  
The CCD you selected is installed in your camera.  
Keep all the original packing materials so you can safely ship the CoolSNAP  
system to another location or return it for service if necessary.  
If you have any difficulty with any step of the instructions, call Photometrics  
Customer Service.  
The CoolSNAP package includes an image-capture software program designed  
for use with your CoolSNAP camera.  
Software  
Compatibility  
Requirements  
All other imaging software must also be PVCAM-compatible. For full access to  
imaging software functions, the most current version of PVCAM must be used.  
The host computer (PC) for your CoolSNAP camera must have the following:  
Host Computer  
Requirements  
®
Windows 2000 or XP operating system  
®
1 GHz Pentium 4 (or greater)  
256 MB RAM (or greater)  
CD-ROM drive  
At least one unused PCI or PCI-X card slot  
16-bit color display (or greater)  
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If you are a Mac user, the host computer for your CoolSNAP camera must have  
the following:  
Macintosh OS X.3 (Panther) operating system  
512 MB RAM (or greater)  
CD-ROM drive  
At least one unused PCI or PCI-X card slot (LVDS cameras only)  
At least one unused IEEE-1394 port (IEEE-1394 cameras only)  
Video adapter that supports 24-bit color (millions of colors)  
Windows versions of PVCAM support multiple open LVDS cameras. In order to  
use this function, it must also be supported by your imaging software. Many  
imaging packages support multiple open cameras.  
Multiple Cameras  
If your imaging software supports multiple cameras, there must be a separate  
PCI card for each camera.  
PVCAM for Macintosh, PVCAM for Linux, and IEEE-1394 cameras do not  
support the multiple open camera feature.  
An Installation Guide appropriate to your system is included with your camera  
system. This guide provides step-by-step instructions for installing the camera  
interface software and the application software for Windows-based and  
Macintosh-based PCs. Additional instructions are included for installing a PCI  
card in your computer and capturing images.  
Software  
Installation  
The Photometrics CD-ROM contains the following files.  
Linux directory — this directory contains the files for installing on a  
Linux PC.  
MacOS directory — this directory contains the files required for  
installing on a Macintosh computer.  
Manuals directory — this directory contains user manuals in PDF  
format.  
WinOS directory — this directory contains the files for installing on a  
Windows PC.  
You will be using a CoolSNAP interface card to allow the camera to  
communicate with your computer.  
Installing the  
Interface Card  
Refer to the Readme text files on the CD-ROM and to the Software Installation  
insert before installing the interface card.  
After installing the interface card, proceed to Connecting your CoolSNAP Camera  
on page 7.  
6
CoolSNAPcf /CoolSNAPES  
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The CoolSNAP cable connects your CoolSNAP camera to the interface card. This  
cable is designed to serve as a conduit both for data and power.  
Connecting Your  
CoolSNAP  
Camera  
CoolSNAP LVDS Cable  
CoolSNAP IEEE-1394 Cable  
To connect your LVDS CoolSNAP camera:  
1. Connect either end of the CoolSNAP LVDS cable to the CoolSNAP interface  
card that you have installed in the host computer.  
2. Connect the other end of the CoolSNAP LVDS cable to the DATA connector  
located on the back of the camera (shown below).  
The following connectors and display lights, as well as the power switch, are  
located on the back of the CoolSNAP camera.  
DATA connector: 20-pin, high-density connector for data transfer and  
power.  
EXPOSE OUT connector: BNC connector; the signal at the BNC  
connector will go to a TTL high level when the exposure begins; useful  
for synchronizing an external shutter in the illumination pathway; will  
not power the shutter.  
XFER display light: amber LED illuminates during data transfer.  
ON display light: green LED illuminates when camera is powered on.  
POWER switch: momentary on/off rocker switch (CoolSNAPcf ) or  
standard rocker switch (CoolSNAPES).  
Note: LVDS CoolSNAP cameras draw power from the PCI bus via the PCI card.  
Therefore, the computer must be powered on for the LVDS CoolSNAP camera to  
operate.  
Chapter 2. System Installation  
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To connect your IEEE-1394 CoolSNAP camera:  
1. Connect either end of the CoolSNAP IEEE-1394 cable to the CoolSNAP  
IEEE-1394 interface card that you have installed in the host computer.  
2. Connect the other end of the CoolSNAP IEEE-1394 interface cable to either  
DATA connector located on the back of the camera (shown below).  
The following connectors and display lights are located on the back of the  
CooSNAP camera.  
DATA connectors: 6-pin IEEE-1394 connectors for data transfer and  
power.  
EXPOSE OUT connector: BNC connector; the signal at the BNC  
connector will go to a TTL high level when the exposure begins; useful  
for synchronizing an external shutter in the illumination pathway; will  
not power the shutter.  
XFER display light: amber LED illuminates during data transfer.  
PWR display light: green LED illuminates when camera is powered on.  
EXT PWR connector: 5.5 x 2.1 mm DC jack for auxiliary CoolSNAP  
power supply.  
Note: IEEE-1394 CoolSNAP cameras draw power from the IEEE-1394 bus. Therefore, the  
computer must be powered on for the IEEE-1394 CoolSNAP camera to operate.  
8
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Chapter 3.  
Operating Features  
Common Features  
The features described in this section are identical in the CoolSNAPcf and  
CoolSNAPES cameras. Features that are specific to the CoolSNAPES camera are  
described separately in the "CoolSNAPES" section.  
Readout Speed  
Binning  
The CoolSNAPcf and CoolSNAPES cameras operate at 20 MHz digitization and  
offer greater than 10 full frames per second.  
Both cameras support 1x, 2x, 3x, 4x, and 8x binning in the serial (horizontal)  
direction and flexible binning in the parallel (vertical) direction. Binning  
increases the frame rate and sensitivity but at the expense of resolution.  
Choosing a smaller region of interest (ROI) further increases the frame rate.  
The following subsections describe features that are specific to CoolSNAPES  
camera systems.  
CoolSNAPES  
Features  
Dual Mode Operation  
A unique feature of CoolSNAPES is that it allows two different CCD clocking  
modes that allow you choose between speed and NIR sensitivity. In the PVCAM  
implementation, the clocking modes are referred to as "normal" and "alternate  
normal".  
Normal mode In "Normal" mode, the CCD is optimized for maximum anti-blooming protection  
and frame rate. In this mode, the CCD can be clocked so that exposure and readout  
happen simultaneously as shown in the example below.  
Example: Consider a situation where the full frame readout time is 90 msec (at  
20 MHz) and the exposure time is 200 msec. The readout of a frame will occur  
during the exposure of the next frame. This is possible because the CCD has  
alternate columns of sensitive and masked areas. While charge is integrating in the  
sensor area, the previous frame, which is in the masked area, can be read out  
(Figure 1). In this example, the time required to acquire the three-image sequence is  
690 ms (3 x 200 + 90) and the frame rate is approximately 4.3 fps.  
200ms  
200ms  
200ms  
Exposure  
Readout  
90ms  
90ms  
90ms  
Figure 1. Normal Mode, Overlapped  
9
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If the exposure time is 50 ms, which is less than the readout time, the camera  
operates in "nonoverlapped" mode (see Figure 2). The effective frame rate for  
this is 7.14 fps (1/[0.05+0.090]). While in normal mode, the camera firmware  
automatically calculates the readout times, taking into account binning and/or  
subregion, and carries out the exposure-readout sequence to maximize the  
frame rate.  
50ms  
50ms  
50ms  
50ms  
50ms  
50ms  
Exposure  
Readout  
90ms  
90ms  
90ms  
90ms  
90ms  
Figure 2. Normal Mode, Nonoverlapped  
Alternate Normal mode  
In "Alt-Normal", it is possible to achieve higher QE in the NIR (peak QE of  
approximately 65%) by manipulating the CCD clock voltages. Also, in this  
high-sensitivity mode, the preamplifier is switched off during the exposure to  
eliminate the background generated by preamplifier glow. In this clocking  
mode, irrespective of what the exposure time is, the camera operates in  
"nonoverlapped" or "sequential" mode.  
70  
60  
alternate-normal - high sensitivity  
50  
40  
30  
20  
normal - high frame rate  
10  
0
200 300 400 500 600 700 800 900 1000 1100  
Wavelength (nm)  
Figure 3. QE for Normal and Alternate Normal Modes  
Antiblooming  
Typically, interline CCD devices are designed with antiblooming capabilities. To  
prevent excess electronic charge from migrating to adjacent pixels, "drains" are  
built into the CCD. These drains remove any excessive charge generated from an  
overexposed pixel. Sony interline CCDs will prevent blooming for optical  
signals greater than 1000 times the full-well capacity of a single pixel. However,  
the extended QE capabilities of the Sony ICX285 in the CoolSNAPES reduce the  
antiblooming suppression for certain modes. In normal mode, the CCD operates  
with typical antiblooming suppression. But in alternate normal mode, the  
enhanced sensitivity causes a reduction in antiblooming to greater than 100  
times single-pixel full-well capacity.  
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Application Examples  
Example 1  
"I have a CoolSNAPES and want to operate the camera in the most sensitive  
setting for taking high-resolution, single images."  
For this application, the camera should be operated in "alternate normal" mode  
to provide the best quantum efficiency and the camera gain should be set to 2.  
These settings will operate the camera in its most sensitive mode.  
Example 2  
"I would like to acquire sequences of images with a CoolSNAPES to study time-  
correlated phenomena. My light level is fairly high and I want to optimize the  
acquisition rate of the camera."  
First, the camera speed should be set to 20 MHz. In addition, the camera should  
be put into "normal" mode to take advantage of the overlapping of the readout  
with the integration time. Finally, the "clearing" mode of the camera should be  
set to "clear pre-sequence" to remove the clearing overhead between frames. Of  
course, reducing the region of interest and increasing binning will always  
increase the frame rate further.  
Chapter 3. Operating Features 11  
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Chapter 4.  
Troubleshooting  
If you have any difficulty while troubleshooting, or do not see your camera system’s  
symptoms listed here, contact Photometrics Customer Service.  
If your operating system does not boot normally after you have installed a  
interface card, try installing the new card in another open interface slot. If this  
does not work:  
System Does Not  
Boot Normally  
1. Turn off your computer and remove the newly installed interface card.  
2. Turn your computer back on. If your system boots normally, there is  
probably an interrupt conflict between a previously installed expansion card  
and the interface card that you are installing.  
3. If you need assistance resolving the interrupt conflict, contact Photometrics  
Customer Service.  
If the New Hardware Found dialog box does not appear after installing a new  
interface card to your computer and booting Windows 2000/XP:  
New Hardware  
Found Dialog Box  
Does Not Appear  
(Windows  
Check to make sure that the new interface card is inserted in a PCI slot  
according to your computer manufacturer’s instructions and that the  
Photometrics disc is in the host computer’s CD drive.  
2000/XP)  
It is possible that there is a conflict between the new interface card and a  
previously installed expansion card. With the computer’s power turned off,  
remove any previously installed expansion cards that your system does  
not need to function. (If you are unsure which cards can be safely  
removed, call Photometrics Customer Service.) Then turn your  
computer back on and boot Windows 2000/XP again.  
If the New Hardware Found dialog box still does not appear, contact  
Photometrics Customer Service.  
If the green LED on the back of the camera does not illuminate when the power  
switch is pressed to the ON position:  
Green LED Does  
Not Illuminate  
(Camera Has No  
Power)  
Check to make sure that the host computer has power:  
If it does not, power the computer on and then switch on the  
camera.  
If it does, power off the computer, check all system connections  
(particularly both ends of the data cable), power the computer back on,  
and then switch on the camera again.  
If you are using a CoolSNAP camera with an IEEE-1394 interface,  
connect only one camera at a time to the IEEE-1394 interface card.  
If the camera still does not have power, contact Photometrics  
Customer Service.  
12  
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If the amber LED on the back of the camera does not illuminate while images are  
being acquired and no images appear:  
Images Not  
Displayed  
Properly  
Confirm that the green LED on the back of the camera is illuminated,  
indicating that the camera is powered on. See Green LED Does Not  
Illuminate (Camera Has No Power) on page 12.  
Confirm that the correct CoolSNAP camera is selected in your imaging  
software application.  
Power off the camera and the host computer and check all system  
connections (particularly both ends of the data cable). Restart. If the  
amber LED on the back of the camera does illuminate while images are  
being taken, but no images appear:  
Confirm that Windows is set for at least 16-bit colors.  
Confirm that the camera is operational by taking an image with a  
standard C-mount lens attached to your CoolSNAP. Using normal  
room lighting, place the camera on a table about 3 meters away  
from an object and acquire an image using your system’s Brightfield  
settings.  
If the problem persists, contact Photometrics Customer Service.  
If you notice bright spots (hot pixels) in the image or an increase in background  
noise, take another calibration image and then re-acquire the original image.  
Bright Spots in  
Image / Increased  
Background Noise  
Camera Running  
Too Warm  
It is normal for the camera to be slightly warm to the touch while in operation.  
However, if the camera is more than slightly warm to the touch (and at least 1/2  
inch of space has been left around the external cooling fins for air flow), switch  
off the camera immediately and contact Photometrics Customer Service.  
If a PVCAM error message appears, note the message’s number code and  
contact Photometrics Customer Service.  
PVCAM Error  
Message Appears  
If you notice lengthy pauses marked by a lot of disk activity while imaging:  
Lengthy Pauses  
During Imaging  
Close any other programs that may be running.  
Install more physical memory to your computer system.  
13  
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Some high-end Windows 2000/XP-enabled computers with Intel Pentium or  
Xeonprocessors include a memory enhancement called Physical Address  
Extension (PAE). PAE allows these compters to address memory above 4GB;  
however, when PAE is enabled the IEEE-1394 CoolSNAP camera will not  
transmit any pixel data.  
IEEE-1394  
CoolSNAP  
Camera will Not  
Image When  
Attached to  
Certain Dual-  
processor or  
Hyperthreading-  
capable PCs  
To determine whether your computer is running with PAE enabled, go to the  
Control Panel and open the "System" icon. Under the "General" tab, you will  
find a reference to Physical Address Extension in the "Computer" section.  
To disable PAE, you must edit your C:\boot.ini file and remove the /PAE flag.  
14 CoolSNAPcf /CoolSNAPES  
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Chapter 5.  
Basic Specifications  
Dimensions  
4.50 in.  
(11.43 cm)  
2.52 in.  
(6.40 cm)  
C
L
Tripod Mount Hole  
on center with CCD  
(.25 in. - 20 UNC-2B)  
CoolSNAP Camera: Front View  
Tripod Mount Hole  
(centered on  
face of camera)  
2.10 in.  
(5.33 cm)  
5.01 in.  
(12.72 cm)  
5.57 in.  
(14.15 cm)  
CoolSNAP LVDS Camera: Side View  
15  
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CoolSNAP IEEE-1394 Camera: Side View  
16 CoolSNAPcf /CoolSNAPES  
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CCD  
Specifications  
and Orientations  
CoolSNAPcf  
CoolSNAP  
ES  
Image Type  
Array  
Color and Mono  
Mono  
Sony ICX205AK (color)  
Sony ICX205AL (mono)  
Sony ICX285AL  
Resolution  
Pixel Size  
1392 x 1040  
1392 x 1040  
4.65 µm x 4.65 µm  
6.45 µm x 6.45 µm  
20 MHz  
Digitization Rate 20 MHz  
Readout Noise  
Cooling  
15 e- rms @ 20 MHz  
8 e- rms at 20 MHz  
Thermoelectric, 5°C below  
Thermoelectric, 5°C below  
ambient temperature  
ambient temperature  
y
y
=
=
11040  
1040  
(0,0)  
x = 1392  
.25 in.  
(.63 cm)  
CCD Orientation, LVDS Camera  
CCD Orientation, IEEE-1394 Camera  
Camera weight: 1.9 lb. (863 g)  
Additional  
Measurements  
Tripod mounting hole (bottom of camera): .25 in.-20 UNC-2B  
Safe tripod mounting screw length: .25 in. (.63 cm)  
Safe C-mount depth: .47 in. (11.9 mm)  
Flange focal distance: .69 in. (17.5 mm)  
17  
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Detector  
Rear Panel Description  
The following connectors, display lights, and controls are located on the back of  
the cameras (pictured below).  
DATA connector: for data transfer and power  
LVDS cameras: 20-pin, high-density connector  
IEEE-1394 cameras: 6-pin IEEE-1394 connector  
EXPOSE OUT connector: BNC connector; the signal at the BNC connector will  
go to a TTL high level (+ 5 V) when the exposure begins; useful for  
synchronizing an external shutter in the illumination pathway; will not power  
the shutter  
XFER display light: amber LED illuminates during data transfer  
ON display light (LVDS camera)/PWR display light (IEEE-1394 cameras):  
green LED illuminates when camera is powered on  
POWER switch (LVDS cameras only): standard rocker switch  
EXT PWR connector (IEEE-1394 cameras only): 5.5 x 2.1-mm DC jack for  
auxiliary CoolSNAP power supply  
CoolSNAP LVDS Rear Panel  
CoolSNAP IEEE-1394 Rear Panel  
18 CoolSNAPcf /CoolSNAPES  
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Auxiliary Power  
Supply  
Usage Requirements The auxiliary CoolSNAP power supply (shown below) provides power to your  
CoolSNAP camera, but is required only under certain conditions.  
If your CoolSNAP camera is connected to an IEEE-1394 port that does not  
supply power (such as a 4-pin connector on a laptop computer) you need to use  
the auxiliary power supply.  
The data ports on the CoolSNAP camera do not pass power. If the CoolSNAP  
camera is connected in a daisy chain configuration, and it is not the first device  
in the daisy chain, you need to use the auxiliary power supply.  
If the CoolSNAP camera is connected to a PC that supplies power through the  
6-pin IEEE-1394 port, the auxiliary power supply is not needed; however,  
connecting the power supply will not damage the camera.  
Warning: Use the auxiliary power supply that shipped with your system ONLY. Do  
not use third-party power supplies.  
Installation The auxiliary power supply ships with four unique wall plugs. You need to  
install one of the plugs on the power supply before using it. Select the correct  
plug for your wall socket and press it into the power supply. The supplied  
plugs include:  
NEMA 1-15 for North America/Japan  
BS1363/A for United Kingdom  
EN50075 for Europe  
AS/NZS 3112-1993 for Australia/New Zealand  
Specifications Input voltage: 90-264 VAC/47-63 Hz  
Output voltage: 12 VDC  
Output current (maximum): 1250 mA  
Chapter 5. Basic Specifications 19  
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Index  
Mount  
camera · 3  
A
Multiple cameras · 6  
Alternate normal mode · 10  
Antiblooming · 10  
Application examples · 11  
Auxiliary power supply · 19  
N
Normal mode · 9  
C
O
Camera cable  
ON display light · 7, 18  
installation · 7  
Optional service contract · iv  
Camera description · 1  
Camera features · 9  
CCP orientation · 17  
Cleaning  
P
POWER switch · 7  
Precautions · 2  
exterior camera surfaces · 3  
imaging window · 3  
Clocking modes  
alternate normal · 10  
normal · 9  
R
Repairs · 3  
Restricted rights · iv  
Connecting  
S
your CoolSNAP IEEE-1394 camera · 8  
your CoolSNAP LVDS camera · 7  
Connectors and display lights · 7, 18  
Customer service · 4  
Service Contract (optional) · iv  
Software compatibility requirements · 5  
Specifications · 17  
System components · 1  
D
T
DATA connector · 7, 18  
Declaration of Conformity · v, vi  
Dimensions · 17  
Tripods · 3  
Troubleshooting · 12–13  
Display lights and connectors · 7, 18  
U
U.S. Government Restricted Rights · iv  
E
Examples of applications · 11  
EXPOSE OUT connector · 18  
V
Video output · 11  
H
W
Hardware installation  
camera cable · 7  
Host computer requirements · 5  
Warranties  
normal wear item disclaimer · i  
one year · i  
one year on refurbished/discontinued products · i  
owner's manual and troubleshooting · ii  
software · ii  
I
IEEE-1394 cable · 1  
IEEE-1394 interface card · 1  
vacuum integrity · i  
x-ray detector · i  
your responsibility · ii  
Website · 4  
L
Lenses · 3  
LVDS cable · 1  
LVDS interface card · 1  
X
XFER display light · 7, 18  
M
Microscopes · 3  
20  
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BENELUX  
Roper Scientific, BV  
Ir. D.S. Tuijnmanweg 10  
4131 PN VIANEN, Netherlands  
tel: 31.347.324989  
fax: 31.347.324979  
FRANCE  
GERMANY  
Roper Scientific, SARL  
Z.I. Petite Montagne Sud  
4, rue de l'Oisans - C.E. 1702  
91017 Evry Cedex, France  
tel: 33.160.86.03.65  
Roper Scientific, GmbH  
Rosenheimer Landstr. 87  
D-85521 Ottobrunn, Germany  
tel: 49.89.660.779.3  
fax: 49.89.660.779.50  
fax: 33.160.86.07.09  
JAPAN  
USA  
Photometrics  
3440 East Britannia Drive  
Tucson, Arizona 85706  
tel: 800.874.9789 or 520.889.9933  
fax: 520.295.0299  
Nippon Roper, K.K.  
Sakurai Building,  
2-8-19 Fukugawa  
Koto-ku, Tokyo  
Japan 135-0033  
tel: 81.3.5639.2731  
fax: 81.3.5639.2775  
57-061-001 Rev G0  
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