Leica Microscope Magnifier MS5 User Manual

Leica M Stereo-  
microscopes  
User Manual  
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Dear User  
Thank you for choosing our products. We hope that you will en-  
joy the quality and performance of Leica Microsystems products.  
In developing our instruments, we have placed great emphasis  
on simple, self-explanatory directions. In order to utilize all the  
benefits of your new stereomicroscope, we suggest studying  
this user manual in detail. Should you have any questions, please  
consult your local Leica representative. You will find the address  
of the closest local representative as well as valuable informa-  
tion about products and services from Leica Microsystems on  
We are gladly at your service. Customer service is a big thing  
with us. Not only before the sale, but afterwards as well.  
Leica Microsystems (Switzerland) Ltd  
Stereo & Macroscope Systems  
User Manual  
Your instrument is accompanied by a printed English user man-  
ual. Additional language versions and information can be found  
on the interactive CD-ROM. User manuals and updates are  
available for download on our homepage at  
This user manual contains an explanation of the safety regula-  
tions, assembly, handling and accessories of the Leica MS5,  
MZ6, MZ75, MZ95, MZ125, MZ16, MZ16 A and MZ16 FA  
(if identical) stereomicroscopes. The special functions of the  
automated stereomicroscopes Leica MZ16 A and MZ16 FA can  
be found in separate user manuals.  
Leica M Stereomicroscopes User Manual  
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Table of contents  
Page  
Overview  
Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6  
Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9  
Controls and functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10  
Use  
Changing the magnification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12  
Ergonomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14  
Interpupillary distance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15  
Eyepoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15  
Eye contact, eyecups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16  
Focusing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16  
Microscope carrier: Stereoscopic and axial observation . . . . 18  
Diopter settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20  
Assembly  
Overview: Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22  
Stand  
Profile column for incident light and transmitted-light stands . . 23  
Transmitted-light stand, bright field, 20 W . . . . . . . . . . . . . . . . . . 23  
Transmitted-light stand, bright and dark field . . . . . . . . . . . . . . . 26  
Transmitted-light stand HL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27  
Swinging-arm stand ESD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28  
Large swinging-arm and table-clamp stands . . . . . . . . . . . . . . . 30  
Optics carrier, optical accessories  
Spacer rings, objective combinations . . . . . . . . . . . . . . . . . . . . . 32  
Microscope carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34  
Optics carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34  
Binocular tubes, optical accessories . . . . . . . . . . . . . . . . . . . . . . 35  
Objective nosepiece (MZ16 and MZ16 A) . . . . . . . . . . . . . . . . . . 36  
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Page  
Illuminators  
Transformers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38  
Incident lamp 6 V/10 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40  
Incident lamp 6 V/20 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42  
Cold light sources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45  
Fiber-optic light guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46  
LED illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46  
Supply (line) lamp 25 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47  
Coaxial illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47  
Near-vertical illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48  
Accessories  
Fitting accessory tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50  
Double-iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50  
Drawing tube, discussion tube . . . . . . . . . . . . . . . . . . . . . . . . . . . 51  
Video/phototubes:  
Photography, video, TV, filming, digital imaging . . . . . . . . . . . . . 52  
Attachment for vertical and oblique observation . . . . . . . . . . . . 56  
Graticules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56  
Stages, polarization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57  
Special notes  
Tips and hints: What to do if . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58  
Care and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59  
Calculating total magnification and field  
of view diameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61  
Optical data for MS5, MZ6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62  
Optical data for MZ75, MZ95 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64  
Optical data for MZ125, MZ16, MZ16 A . . . . . . . . . . . . . . . . . . . . 66  
Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67  
Technical data electronical devices . . . . . . . . . . . . . . . . . . . . . . .74  
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Safety concept  
Read the user manual and safety instructions before starting up.  
General directions  
Permitted uses  
The Leica MS5, MZ6, MZ75, MZ95, MZ125, MZ16 and MZ16 A stereomicro-  
scopes are optical instruments which use magnification for improved  
visualization of objects, their details and specimens from technology and  
natural sciences. The basic outfit, which includes a stand and a light  
source, can be supplemented with various modules for photography, TV,  
dual station viewing and other applications.  
• The use of the instrument in a different manner from that described in  
this user manual can lead to injury, malfunction and damage.  
Prohibited  
uses  
• The Leica MS5, MZ6, MZ75, MZ95, MZ125, MZ16 and MZ16 A stereo-  
microscopes must not be used for eye examinations and operations.  
• Do not fit different plugs. Do not dismantle optical systems or mechani-  
cal parts unless instructions for doing so are given in the user manual.  
• The Leica MS5, MZ6, MZ75, MZ95, MZ125, MZ16 and MZ16 A stereo-  
microscopes are intended mainly for indoor use.  
Place  
of use  
• If the stereomicroscope is used outdoors, protect it from dirt and mois-  
ture. Lamps and electrically-operated stands from Leica may not be  
used outdoors.  
The optics carriers MS5, MZ6, MZ75, MZ95, MZ125, MZ16 and MZ16 A, the  
binocular tube, ErgoTube™, ErgoWedge™ 5°–25°, the swinging-arm  
stand ESD and the cold light sources Leica L2 and CLS consist of ESD-  
Use in ESD  
protected rooms  
11  
discharging material (surface resistance <10 ohms/squared, discharge  
time <2 seconds, 1,000 V to 100 V).  
– A connection socket for a 4 mm grounding cable is provided on the  
microscope carrier.  
– There are two connection sockets for 4 mm grounding cables in the  
base of the swinging-arm stand.  
The Leica M series can be used in clean rooms without any problems and  
they can be cleaned as described on page 60. Please observe the  
following rules:  
Use in Clean Rooms  
– Do not clean our stereomicroscopes and accessories in a different  
manner from that described in this user manual. Do not use unsuitable  
cleaning agents, chemicals and techniques. Do not clean painted  
®
surfaces and accessories with rubber parts such as the ErgoTube  
10° – 50° with chemicals.  
This can damage the surfaces. Particles or grindings can contaminate  
the specimen.  
– If the customer cleans our instruments with chemicals without our  
written approval he will do it at his own risk.  
– In most cases we can offer special solutions on request. Some  
products can be modified or we could recommend other accessories  
for use in clean rooms.  
6
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Safety regulations  
Servicing Repairs may only be carried out by Leica-trained service tech-  
nicians.  
Only original Leica spare parts may be used.  
Responsibilities • Ensure that personnel who use the instrument have read and  
of person in charge  
of instrument  
understood this user manual and in particular the safety  
instructions.  
• Ensure that the Leica MS5, MZ6, MZ75, MZ95, MZ125, MZ16  
and MZ16 A stereomicroscopes are operated, maintained  
and repaired only by  
authorized and trained personnel.  
Workplaces with stereomicroscopes facilitate and improve  
the viewing task, but they also impose high demands on the  
eyes and holding muscles of the user. Dependent upon the  
duration of uninterrupted activity, this may lead to asthenic  
trouble and muscular-skeletal pain so that suitable measures  
must be taken to reduce the strain:  
– perfect design of workplace (see p.14), work content and  
work process (frequently changing activities).  
– detailed introduction of the personnel while considering  
ergonomic and organizational work-related aspects.  
The ergonomic optics concept and the design of the Leica M  
series aim at limiting the strain on the user to the lowest possi-  
ble level.  
Direct contact with eyepieces can be a potential transmission  
path for bacterial and viral infections of the eye. Users should  
be made aware of the potential risk of infection. The risk can be  
kept at a minimum by using individual eyepieces or attachable  
eyecups (see p.16).  
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Electrically-operated items  
The safety regulations listed below apply to the following items:  
– Transmitted-light stand, bright field, 20 W (page 24)  
– Supply (line) lamp 25 W (page 47)  
– Regulating transformer 0–7 V/40 W (page 38)  
– Step transformer 4/5/6 V (page 39)  
– Motor focus (separate instructions)  
– Motorized Leica MZ16 A  
Liquids Be careful when handling liquids.  
If spilt on the equipment, they can:  
– cause the stereomicroscopes and accessories to become  
electrically live, endangering personnel,  
– cause damage to the equipment.  
Power cables Inspect cables regularly for damage. Defective cables can:  
– directly endanger personnel,  
– cause the stereomicroscopes and accessories to become  
electrically live, endangering personnel.  
Cable routing Route cables with care. Make sure that personnel do not get  
entangled with them. The instrument could fall over, fall down,  
become damaged, damage other equipment, or cause personal  
injury.  
Opening the instrument Only authorized Leica personnel are permitted to repair electri-  
cal equipment. Before opening up the equipment, pull out the  
power cable. If you touch the live interior of the equipment, you  
may receive an electric shock.  
Supply voltage Ensure that the voltage setting on the equipment is correct.  
A wrong setting can cause damage to the equipment.  
Connections When connecting power-consuming devices to the transform-  
ers, do not exceed the maximum permitted loading, as this can  
cause damage to the equipment.  
Changing bulbs • Before changing a bulb, disconnect the power cable.  
• Never attempt to touch the inside of a lamp housing or  
transmitted-light stand while the instrument is connected  
to the power supply.  
• Before changing a bulb, wait until it is cool, otherwise you  
may burn your fingers.  
8
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Integration in The following must be observed if Leica products are build into  
third-party products third-party products:  
The manufacturer of the complete system or the person putting  
it on the market is responsible for adhering to applicable safety  
regulations, laws and guidelines.  
Legal requirements Adhere to general and local regulations relating to accident  
prevention and environmental protection.  
Disposal The products described here must be disposed off in accor-  
dance with applicable local laws and regulations.  
Conformity with The electrically-operated accessories for the Leica MS5, MZ6,  
European Community MZ75, MZ95, MZ125, MZ16 and MZ16 A stereomicroscopes are  
directive constructed in accordance with the latest technologies and  
provided with a statement of conformity with EC requirements.  
Symbols used in this user manual  
Safety instructions  
This symbol denotes information which must be read and  
obeyed. Non-observance  
– can put personnel at risk!  
– can cause malfunctioning or damage to the equipment.  
Warning about accessible hot places, e.g. bulbs.  
Failure to respect it can cause burns!  
Important information  
This symbol denotes additional information or explanations  
which promote understanding.  
Action  
̈This symbol within the text indicates that certain operations  
must be carried out.  
Explanatory notes  
• This symbol within the text stands for additional information  
and explanations.  
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Overview  
Stereomicroscope  
Binocular tube  
1
2
Magnification changer  
7
Adjustable eyepiece tubes  
Interpupillary distance adjustable from  
52 mm – 76 mm.  
Engaging ratchet positions  
3a Focusing drive  
8
Wide-field eyepieces for persons wearing  
glasses  
Diopter setting adjustable within the range  
from +5 to –5, and eyecups adjustable.  
3b Coarse and fine focusing  
Larger knob: Coarse focusing,  
Smaller knob: Fine focusing.  
3c Adjustable ring  
9
Clamping screws  
These hold the eyepieces in the  
binocular tube.  
Changes the ease of movement  
of the coarse/fine drive.  
4
5
6
Clamping screw  
Holds the optics carrier in the  
microscope carrier.  
10 ErgoTube™  
The viewing angle is adjustable within the  
range 10° – 50°.  
Clamping screw  
Holds the binocular tube (or accessory)  
to the optics carrier.  
11 Built-in double-iris diaphragm  
(MZ16/MZ16 A only)  
The special functions of the Leica MZ16 A are  
shown in the user manual M2-116-0.  
Interchangeable objective  
Achromatic, planachromatic or planapochromatic  
Leica MS5  
Leica MZ6  
8
8
9
7
9
7
5
5
1
1
2
4
6
4
6
3a  
3a  
10  
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Leica MZ75  
8
Leica MZ95  
8
7
5
7
5
2
1
1
2
4
6
4
6
3c  
3b  
3c  
3b  
Leica design  
by Ernest Igl/Christophe Apothéloz  
Leica MZ125  
8
Leica MZ16  
8
9
7
10  
5
7
2
10  
5
1
11  
3b  
1
2
4
6
3c  
4
6
3c  
3b  
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11  
Use  
Magnification changer  
• Zoom means continuous change of magnification.  
Ratchets can be engaged for certain magnifications  
(see below).  
• For zooming with MZ16 A, see the user manual M2-116-0.  
MS5: 5 steps  
MZ6: 6:1 zoom  
MZ75: 7.9:1 zoom  
MZ95: 9.5:1 zoom  
MZ125: 12.5:1 zoom  
MZ16 and MZ16 A: 16:1 zoom  
Changing the • The magnification changer can be operated from either  
magnification  
the right or the left side.  
̈Look into the eyepieces.  
̈Focus on the object (page 16).  
̈Turn the magnification changer  
to the desired position.  
Ratchet steps MZ6: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2  
MZ75: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4  
MZ95: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5  
MZ125: 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8  
MZ16: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8, 10  
• Engage the ratchets whenever magnifications need to be  
exactly reproducible.  
̈For MZ6, MZ95, MZ125 and  
MZ16: Press the switch.  
̈For MZ75: Turn the knurled ring  
counterclockwise.  
For setting ratchets at the MZ16 A,  
see the user manual M2-116-0.  
12  
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Scale of MS5, MZ6: Display of magnification factors from 0.63–4.  
magnifications  
MZ75: Display of magnification factors from 0.63–5.  
Corresponding scales for other objective and eyepiece combi-  
nations can be affixed to the rotary knobs (page 34).  
MZ95: Display of magnification factors from 0.63–6.  
MZ125: Display of magnification factors from 0.8–10.  
MZ16, MZ16 A: Display of magnification factors from 0.71–11.5  
MZ16 A: For the digital display of the magnification see the user  
manual M2-116-0.  
Magnifications The tables on pages 62 – 66 provide information about the  
and fields magnifications and field of view diameters as a function of the  
of view magnification changer position of the eyepiece-objective  
combination used.  
Magnification with When the MZ125/MZ16 planachromatic and planapochromatic  
MZ125/MZ16 objectives are used on the Leica MS5, MZ6, MZ75 and MZ95,  
planachromatic and the magnification is increased by the factor 1.25× . This factor  
planapochromatic is taken into account in the tables.  
objectives  
Magnification on  
MZ125/MZ16  
Magnification on  
MS5/MZ6/MZ75/MZ95  
1× plan/planapo  
1.6× planapo  
0.63× planapo  
0.5× plan  
1.25×  
2×  
0.8×  
0.63×  
1×  
0.8× plan  
2× planapo  
2.5×  
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Ergonomics, viewing height  
Preconditions for • Pay great attention when setting up your stereomicroscope.  
working comfortably  
You will only benefit fully from the outstanding optical and  
ergonomic advantages of the instrument if you set it up  
precisely in accordance with the directions already given.  
• For fatigue-free work, use the ErgoModules™.  
• Arrange your workplace as well as possible, and pay  
attention to the heights of chair and table.  
• When sitting, use the entire seat area and also the back  
support of the chair.  
• Support your forearms.  
• During work breaks, stand up and move around for exercise.  
Please also observe the note on p. 7.  
Ergonomics The reasons why users often cannot work comfortably with  
the 45° tube are the differing heights of the outfits, the various  
accessories and working distances, and the fact that the  
same instrument is often used by several people of differing  
builds. This problem can be effectively solved by using the  
Leica ErgoModules™.  
If you have problems with the viewing configuration on your  
stereomicroscope, ask your Leica consultant for the most  
ergonomic Leica solutions.  
Ergo objective With the achromatic Ergo objective 0.4×– 0.63× for the Leica  
MS5, MZ6, MZ75 and MZ95 it is possible to focus in the region  
of 90mm (63.5 mm – 153.5 mm) ergonomically and precisely,  
without changing the viewing height. At the same time,  
magnification and working distance can be changed without  
any time-consuming changing of the objectives.  
Viewing height, The viewing height is correct when your head and back are  
viewing angle comfortably positioned.  
• On the ErgoTube™, the viewing angle  
is adjustable from 10° – 50°.  
̈Holding the eyepiece tubes at their  
bases, swing them up or down (see  
arrow).  
14  
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ErgoModule™ – ErgoWedge™ 5° – 25°  
– ErgoWedge™ 15°  
– ErgoModule™ 50 mm  
– The ErgoTube™ 45° has eyepiece tubes which are 65 mm  
longer than the standard ones.  
Magnification factor 1.6×.  
MZ16 A: The magnification factor can be toggled on and off  
using the OPT key.  
– ErgoModule™ 30 – 120 mm  
Relubrication may be required  
after several years (please send  
the item to Leica Service).  
Ergo objective The achromatic Ergo objective 0.4×–0.63× can be used to  
ergonomically and accurately focus in the range of 90 mm  
(63.5–153.5 mm) without changing the viewing height. At the  
same time, magnification and working distance can be  
changed without time-consuming change of objectives.  
Motor focus Frequently repeated fine-motor tasks, such as focusing by  
hand, can lead to muscle fatigue and hand problems. Using the  
Leica motor focusing system, every equipment can be moved  
up and down without applying force.  
Interpupillary distance, eye contact  
Interpupillary distance The interpupillary distance is correct  
when you see a single circular field when  
viewing with both eyes.  
̈Look into the eyepieces and use both  
hands to move the eyepiece tubes closer  
together or further apart.  
Locating the exit pupil The distance between eye and eyepiece measures approx.  
22 mm for 10×/21B wide-field eyepieces for persons wearing  
glasses and 12 mm for 10×/21 wide-field eyepieces.  
̈Slowly approach the eyepieces with  
your eyes until you can see the complete  
image field without corner cutting.  
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Selecting the degree You do not wear glasses and prefer close contact with 10×/21B  
of eye contact wide-field eyepieces for persons wearing glasses:  
̈Holding the diopter ring, turn the eyecup  
counterclockwise until it is released.  
̈Pull the eyecup upwards.  
̈Holding the diopter ring,  
turn the eyecup clockwise.  
Persons wearing glasses need the  
whole of the available distance.  
Move the eyecups to the lowest  
position.  
The 10×/21B wide-field eyepieces for persons wearing glasses  
are supplied with soft eyecups, which can be attached to the in-  
tegrated eyecups made of hard plastic to achieve the following:  
Soft  
eyecups  
– Prevent eye infections if several users are working at the  
same instrument (see p. 7).  
– Protect your glasses from being scratched.  
The 10×/21 wide-field eyepieces are supplied with laterally  
tapered soft eyecups.  
Setting the working distance, focusing = obtaining a  
sharp image  
• To focus, use the focusing drive to raise or lower the stere-  
omicroscope until the feature of interest is in focus working  
distance to the objective.  
The working distances of the various objectives are listed in  
the tables on pages 62 – 66.  
• The focusing drive can be operated from either the right or  
the left side.  
• When focusing on a new object, always start with the lowest  
magnification, because the larger field of view makes the  
object easier to find. Afterwards, select the desired magnifi-  
cation.  
16  
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̈Place the object beneath the objective.  
̈Engage the lowest magnification  
(page 12).  
̈Look into the eyepieces.  
̈Using the rotary knob, bring the  
object into focus.  
The coarse/fine drive supports up to  
15 kg.  
Coarse/fine drive  
– Resolution with 5 kg load: 1 µm  
– Resolution with 10 kg load: 2 µm  
̈Focus approximately with the  
larger knob.  
̈Fine-focus with the smaller  
(concentric) knob.  
Is the focus movement too loose or too tight? Does the outfit  
tend to slide downwards? You can adjust the movement in  
accordance with the weight of the outfit and with your own  
personal requirements:  
Adjusting the free  
running of the focusing  
drive  
̈Hold the drive knobs firmly with  
both hands and turn them in  
opposite directions until you  
obtain the required degree of  
resistance during focusing.  
– Focusing drive  
MS5 – MZ95  
̈Hold the left or right fine drive.  
̈Turn the other fine drive until the  
desired resistance during  
– Focusing drive,  
coarse/fine  
focusing is reached.  
Leica M Stereomicroscopes – Operation  
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Microscope carrier  
Microscope carrier • This microscope carrier permits only  
for stereoscopic  
stereoscopic observation, using both beam paths.  
observation • The microscope carrier can be fitted to the drive  
housing in either of two positions (see page 34):  
• The optics carrier can be turned sideways in  
the microscope carrier if the user needs to  
work from the side:  
̈Release the clamping screw.  
̈Turn the optics carrier sideways  
as required.  
̈Retighten the clamping screw.  
Microscope carrier AX  
The stereomicroscopes can be equipped with the microscope  
carrier AX for stereoscopic and axial observation instead of  
the standard microscope carrier (page 34).  
Different models are available for MS5, MZ6, MZ75, MZ95 and  
for MZ125, MZ16 and MZ16 A. The achromatic objectives and  
the planochromatic 1× fit in the thread in the AX microscope  
carrier for MS5, MZ6, MZ75, MZ95 (p. 33).  
The planochromatic and planapochromatic objectives fit in the  
thread in the AX microscope carrier for MZ125, MZ16, MZ16 A  
(p. 33). In addition, the AX microscope carrier features a clamp-  
ing screw for fixing it in one of 3 positions and an aperture which  
can be turned manually (see below). Otherwise, the use of and  
switching to the left, middle and right position are identical.  
• The optics carrier cannot be turned sideways in the micro-  
scope carrier AX.  
Stereoscopic A three-dimensional image is required for examining plastic  
observation objects and for working.  
̈Shift the stereomicroscope to the central position.  
18  
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Axial observation The parallax-free imaging produces more precise results in  
photography, measuring and polarization.  
̈Shift the stereomicroscope to the left  
or the right position. The object is now  
located directly beneath either the left  
or the right beam path.  
• When photographing or measuring, se-  
lect the beam path above which the pho-  
totube or measuring graticule is located.  
Aperture  
A rotating aperture is build into the AX microscope carrier. It  
prevents interfering reflections during axial observation with  
coaxial incident illuminator and quarter-wave plate.  
• With the AX microscope carrier for MS5, MZ6, MZ75, MZ95,  
the aperture automatically turns during the moving process.  
• With the AX microscope carrier for MZ125, MZ16, MZ16 A,  
the aperture is located underneath the microscope carrier  
and must manually be turned as follows:  
Please note:  
– In the position for stereoscopic observa-  
tion through both beam paths, turn the  
aperture counterclockwise until it stops.  
Otherwise the field of view is underex-  
posed (vignetting).  
– In the position for axial observation  
through the right or left beam path, turn  
the aperture clockwise until it stops.  
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19  
Adjusting diopter settings and parfocality  
Tips for your work Leica stereomicroscopes are parfocally matched. This means  
that, once you have focused on a feature in the manner de-  
scribed, taking account of diopter settings, you can zoom the  
magnification up and down without refocusing. You only need  
to refocus if you want to look at higher or lower planes in the  
object. A prerequisite is the correct setting of the diopters and  
the parfocality.  
• The dioptric range is from +5 to –5.  
The following adjustments must be carried out only once by  
each user. The procedures for adjusting the dioptric setting and  
the parfocality are somewhat different if an eyepiece contains  
a graticule. Refer to the user manuals for the MPS modular  
photomicrographic systems or for measuring.  
A Preparations ̈Install the lamp.  
̈Set the interpupillary distance and,  
if necessary, the viewing height.  
̈On the video/phototube: Move the  
lever to position "VIS" and open the  
diaphragm.  
̈Set the double-iris diaphragm to "OPEN."  
̈Adjust the eyecups.  
̈Set both eyepieces to zero.  
̈Set the microscope carrier AX for  
stereoscopic observation.  
B Focus on the 1. Place a flat test object under the  
test object objective.  
2. Engage the lowest magnification  
position.  
̈Use the coarse focusing drive to set  
the working distance (page 16).  
3. Look into the eyepieces  
̈Use the focusing drive, to bring  
the test object into focus.  
20  
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4. Engage the highest magnification position.  
5. Optimize the focus using the focusing drive.  
C Adjusting 6. Engage the lowest magnification  
dioptric settings position.  
7. Do not look into the eyepieces.  
̈Rotate the eyepieces counterclockwise  
in the "+" direction as far as the stop  
(+5 diopters).  
8. Look into the eyepieces  
̈ Slowly rotate each eyepiece  
individually in the "–" direction until  
each eye sees the object sharply  
imaged.  
D Inspecting 9. Engage the highest magnification  
parfocality position.  
10. Refocus if necessary.  
11. Slowly move the magnification  
changer from the lowest magnification  
to the highest.  
The image sharpness must remain  
constant (parfocal). Otherwise,  
repeat the procedure.  
Leica M Stereomicroscopes – Operation  
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Assembly  
The components  
1a Incident-light base with stage plate  
1b Transmitted-light base with glass stage plate  
2a Side-faced column with drive housing, coarse/fine  
2b Side-faced column with drive housing, 300 mm or 500 mm  
3
4
5
6
7
8
Microscope carrier  
Optics carrier  
Interchangeable objective  
Binocular tube  
Wide-field eyepiece for spectacle wearers, with eyecups  
Various ErgoModules™ or accessories for video, photography,  
drawing, fluorescence, coaxial incident light etc.  
7
8
6
4
5
3
2b  
2a  
1a  
1b  
22  
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Stand  
Incident-light stand  
Side-faced column ̈ Remove the stage plate.  
incident-light base ̈ Insert 3 Allen screws into the underside  
of the baseplate and secure them to the  
bottom end of the side-faced column.  
̈ Insert the stage plate.  
To fit the microscope carrier and the stereomicroscope,  
see page 34  
Transmitted-light stand  
Pull the power cable out of the socket in the stand.  
Side-faced column ➜  
transmitted-light base  
̈ Remove the glass stage plate.  
̈ Carefully invert the transmitted-  
light base and remove 7 Allen  
screws from the base.  
̈ Lift off the lower part of the base.  
̈ Reverse the upper part of the base.  
̈ Insert 3 Allen screws into the cover  
plate from beneath and secure  
them to the bottom end of the side-  
faced column.  
̈ Replace and secure the base.  
̈ Insert the glass stage plate.  
̈ Fit the microscope carrier and the  
stereomicroscope as described  
on page 34.  
Technical data see page 74  
Leica M Stereomicroscopes – Assembly  
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Transmitted-light stand, bright field, 20 W  
Follow the safety instructions given on page 7.  
̈ Fit the side-faced column to the transmitted-light base as  
described on page 23.  
Fitting the bulb  
Never touch the bulb when it is hot.  
You may sustain burns.  
• Do not touch the new 6 V/20 W halogen bulb directly with  
your fingers, because finger marks can cause it to crack  
when heated.  
Remove finger marks immediately with a cloth dipped in pure  
alcohol.  
̈ Insert the slider together with the  
bulb socket.  
̈ Hold the new bulb in a cloth and push  
it into position.  
̈ Push the slider carefully but complete-  
ly back along the rail to the stop.  
Filters • The transmitted-light stand contains a KG1 filter.  
• You can insert other 50 mm filters if you need them:  
̈ Pull out the slider.  
̈ Insert the 50 mm filter.  
̈ Push the slider carefully but  
completely back along the  
rail to the stop.  
Starting up The ex-factory setting of the voltage selector is 230 (for volt-  
ages between 200–240 V) and the fuse holder contains two  
160 mAT fuses.  
For 100–120 V power supply:  
̈ Set the voltage selector to 115.  
̈ Fit the enclosed two 315 mAt fuses  
in the fuse holder instead of the  
two 160 mAT fuses (see the section  
"Changing fuses" on the right).  
̈ Connect the power cable to the socket  
of the base and to the power source.  
24  
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Changing fuses Use:  
– Two 160 mAT fuses for voltages between 200–240 V.  
– Two 315 mAT fuses for voltages between 100–120 V  
(see the section "Starting up" on the left).  
̈ Disconnect the power cable from the stand.  
̈ Pull out the fuse holder.  
̈ Fuse at rear: Press out downwards.  
̈ Spare fuse at front: Pull out sideways and  
insert into holder at rear.  
̈ Push the fuse holder back into position.  
Transmitted or On the rear side of the base, there is a  
incident light socket for incident lamps of up to 20 W.  
• Connect an incident lamp to the trans-  
mitted-light stand, if required.  
• Either transmitted light or incident light  
can be switched on.  
̈ For transmitted light: Switch in position I.  
̈ For incident light: Switch in position II.  
̈ Use thumb wheel to adjust brightness.  
Switching on the ̈ On the rear of the stand: Use the thumb  
transformer  
wheel to switch on the transformer and  
to adjust the brightness.  
Disconnection from ̈ Remove the power cable from the power socket.  
mains power supply  
Transmitted light ̈ Switch on transmitted light.  
Transmitted light, Bright field is suitable for examining translucent objects featur-  
bright field ing contrasting structures. The object is directly illuminated  
from below and is seen in its natural colors against a bright  
background.  
̈ For transmitted light, bright field:  
Pull lever forwards.  
Inclined Inclined transmitted light which traverses the object obliquely  
transmitted light will produce effects advantageous for observing small translu-  
cent objects such as foraminifera and fish eggs.  
̈ Move the lever slowly towards the column of the stand until  
the desired effect is obtained.  
Leica M Stereomicroscopes – Assembly  
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Transmitted-light stand, bright and dark field  
The illumination of the transmitted-light stand bright and dark  
field consists of a fiber-optic light guide and a light source.  
Observe the safety regulations from the  
manufacturer of the light source.  
̈ Unplug the fiber-optic light guide from the connection in  
the stand.  
̈ Fit the side-faced column to the transmitted-light base as  
described on page 23.  
̈ Fit the microscope carrier and stereomicroscope as  
described on page 34.  
̈ Attach the fiber-optic light guide at the stand and the  
light source.  
Starting up Before using the light source, read the corresponding user  
manual.  
Transmitted light, Bright field is suitable for examining translucent objects featur-  
bright field ing contrasting structures. The object is directly illuminated  
from below and is seen in its natural colors against a bright  
background.  
̈ Slide handle to the right until the  
stop: bright field.  
Transmitted light, Dark field offers more information for objects poor in contrast  
dark field with rather poorly marked or very fine structures. In this case,  
the light beams penetrate the object at a flat angle. Fine detail  
structures and contours contrast brilliantly and brightly from  
dark background.  
̈ Slide the handle out to the right:  
dark field.  
26  
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Transmitted-light stand HL  
Column Base The column seat contains Allen screws.  
̈ Remove the glass stage plate.  
̈ Invert the transmitted-light base and place it on a table.  
̈ Hold column against the column seat from underneath.  
̈ Insert the Allen screws into the column from the top and  
tighten them.  
̈ Invert the transmitted-light base and insert the glass  
stage plate.  
̈ Fit additional components such as the microscope carrier  
and optics carrier (page 34).  
Illumination Add a very luminous cold light source and a light guide with  
a channel to the transmitted-light stand (TVC, active 9 mm).  
̈ Insert light guide into the base at the rear.  
̈ Connect light guide to the light source.  
Accessory condenser For stereomicroscopes with high apertures such as Leica  
Basis MZ125, MZ16 and MZ16 A with objective 1.6×, the resolution  
can be increased by inserting an accessory condenser.  
̈ Push the accessory condenser into the lens holder at the  
underside of the transmitted-light base and secure it with  
2 Allen screws.  
Use ̈ Turn on the light source according to the manufacturer’s  
user manual and adjust the light intensity.  
̈ Place a transparent object on the glass stage plate and  
focus.  
Tilted mirror The built-in mirror features a clear and an obscured side and  
can be rotated and moved. The obscured side provides a dif-  
fused illumination. The angle of incidence in the specimen  
plane changes depending on the position of the mirror so that  
the transmitted light bright field via oblique illumination can also  
generate an illumination similar to dark field.  
̈ Rotate and move the mirror with the black rotary knob on the  
right side.  
̈ Adjust exact bright field and maximum illumination: move  
mirror to the stand column until it stops.  
Rotate the mirror until it engages in the 45° position.  
Transmitted-light stand Detailed information about setup, operation and use can be  
HL-RC™ found in the separate instructions for use M2-216-2.  
Leica M Stereomicroscopes – Assembly  
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Swinging-arm stand ESD  
The swinging-arm stand ESD is of conducting material (surface  
resistance <1011 Ohm/squared, discharge time <2 seconds from  
1,000 V to 100 V). Two sockets for connecting 4 mm ground  
cables are provided on the base.  
Assembly of column ̈ Insert 4 Allen screws into the base  
base  
from beneath and secure them to  
the end of the column.  
Safety ring column  
The safety ring secures the instrument and must always  
be fixed beneath the cross-member.  
̈ Slide the safety ring  
down the column.  
̈ Retighten the clamping screw.  
Cross-member ̈ Lower the cross-member onto the  
column  
safety ring.  
̈ Retighten the clamping screw.  
Horizontal arm ̈ Slide the horizontal arm into the  
cross member  
cross-member with the connecting  
piece towards you.  
̈ Retighten the clamping screw.  
Drive housing • The drive housing / microscope carrier can be fitted to  
horizontal arm  
the horizontal arm in either of two positions (see page 34).  
• The circular port in the microscope  
carrier accepts an illuminator.  
̈ Insert the peg of the inclinable focusing  
drive into the connecting piece.  
̈ Retighten the clamping screw.  
Optics carrier ̈ Fit the optics carrier in the  
microscope carrier  
microscope carrier.  
̈ Turn the optics carrier sideways in  
the microscope carrier as required.  
̈ Retighten the clamping screw.  
28  
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Focusing The focusing knobs are located on the inclinable drive housing.  
Is the focus movement too loose or too tight? Does the outfit  
tend to slide downwards? You can adjust the movement in  
accordance with the weight of the outfit and with your own  
personal requirements:  
̈ Hold the drive knobs firmly with both  
hands and turn them in opposite direc-  
tions until you obtain the required degree  
of resistance during focusing.  
Possible adjustments  
Tilting ̈ Release the clamping screw.  
̈ Tilt the instrument.  
̈ Retighten the clamping screw.  
Altering the ̈ Release the clamping screw.  
working distance ̈ Raise or lower the cross-member as re-  
quired together with the horizontal arm.  
̈ Retighten the clamping screw.  
Reposition the safety ring (see page 28).  
Sideways movement ̈ Release the clamping screw.  
̈ Move the instrument sideways  
on the cross-member.  
̈ Retighten the clamping screw.  
Altering the overhang ̈ Release the clamping screw.  
̈ Displace the horizontal arm.  
̈ Retighten the clamping screw.  
Leica M Stereomicroscopes – Assembly  
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Large swinging-arm and table-clamp stands  
Table-clamp stand ̈ Use the clamp to secure the column to  
a table top 20 mm – 50 mm thick.  
Swinging-arm stand ̈ Insert 4 Allen screws into the base from  
beneath and secure them to the end of  
the column.  
Safety ring column  
The safety ring secures the instrument and must always  
be positioned beneath the horizontal arm.  
̈ Position the safety ring with the screw head uppermost and  
towards you, and slide it down the column.  
̈ Retighten the clamping screw.  
Safety ring column ̈ Lower the horizontal arm onto the safety  
ring, with its end pointing towards you.  
̈ Retighten the clamping screw.  
Limitation  
The screw head must engage the notch. It  
limits the lateral movement. It may not be  
removed, and neither may the safety ring  
be milled down.  
If the horizontal arm is swung out beyond  
the limit, the instrument may fall over.  
Drive housing ➜  
horizontal arm  
This section describes the assembly of the focusing drive with  
side-faced carrier rod. To fit the focusing drive with peg, refer  
to page 28.  
̈ Release the clamping lever.  
̈ Tilt the carrier rod downwards.  
̈ Retighten the clamping lever.  
̈ Slide the drive housing with side-faced  
carrier rod onto the horizontal arm.  
̈ Retighten the clamping lever.  
The clamping levers can be positioned  
as required.  
̈ Pull out the clamping lever along  
its axis and turn it.  
30  
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Microscope carrier ➜  
̈ Secure as described  
on page 34.  
drive housing  
Optics carrier ➜  
microscope carrier  
̈ Secure as described on page 34.  
Possible adjustments  
Tilting  
̈ Release the clamping lever.  
̈ Tilt the instrument.  
̈ Retighten the clamping lever.  
Focusing with  
coarse/fine drive  
̈ Set large distances with the larger  
knob.  
̈ Fine-focus with the smaller (concentric)  
knob.  
̈ Using the ring on the right-hand drive  
knob, adjust the ease of movement  
of the drive.  
Altering the working  
distance  
̈ Release the clamping screw.  
̈ Raise or lower the  
horizontal arm.  
̈ Retighten the clamping screw.  
Reposition the safety ring (see page 30).  
Sideways movement  
Altering the overhang  
̈ Release the clamping screw.  
̈ Pull out or push in the  
horizontal arm.  
̈ Retighten the clamping screw.  
During the sideways movement,  
observe the limit stops (see page 30).  
̈ Release the clamping screw.  
̈ Displace the horizontal arm.  
̈ Retighten the clamping screw.  
Leica M Stereomicroscopes – Assembly  
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Optics carrier, optical accessories  
Spacer rings Spacer rings are required in order to attach the objectives for  
certain instrument configurations.  
• The Leica MZ95 is supplied with a spacer ring (10 446 393)  
for the smaller-diameter achromatic objectives and 1×  
planachromatic objective. When the spacer ring has been  
removed by applying the tool provided, the larger-diameter  
planachromatic and planapochromatic objectives for the  
MZ125/MZ16 can be used.  
• The Leica MS5, MZ6 and MZ75 can also be equipped with the  
larger-diameter planachromatic and planapochromatic objec-  
tives for the MZ125/MZ16 if a spacer ring is fitted (10 446 172).  
• When the MZ125/MZ16 planachromatic and planapo-  
chromatic objectives are used on the Leica MS5, MZ6, MZ75  
and MZ95, the magnification is increased by the factor 1.25×.  
10 447 103 MZ16 A  
10 447 102 MZ16  
10 446 370 MZ125  
10 446 272 MZ95  
10 446 371 MZ75  
10 447 107  
10 445 614 MZ6  
10 445 613 MS5  
10 445 819 1x Plan  
10 446 157 0.5x Plan  
10 447 075 0.8x Plan  
10 447 157 1x Planapo  
10 447 101 2x Planapo  
10 447 050 1.6x Planapo  
10 447 051 0.63x Planapo  
Plan/Planapo  
10 411 589 1x  
10 422 562 1.5x  
10 447 081 2x  
10 473 832 0.8x  
10 445 201 0.63x  
10 422 563 0.5x  
10 422 564 0.32x  
Achromat  
10 446 275 1x  
Plan  
10 446 172  
10 447 148  
0.4-0.63x  
Ergo  
10 446 275 1x  
Plan  
10 411 589 1x  
10 422 562 1.5x  
10 447 081 2x  
10 473 832 0.8x  
10 445 201 0.63x  
10 422 563 0.5x  
10 422 564 0.32x  
Achromat  
10 446 157 0.5x Plan  
10 447 075 0.8x Plan  
10 447 157 1x Planapo  
10 447 101 2x Planapo  
10 447 050 1.6x Planapo  
10 447 051 0.63x Planapo  
Plan/Planapo  
10 367 929  
10 315 306  
10 445 352  
10 367 929  
10 315 306  
10 445 352  
32  
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Objective combinations  
Part no.  
MS5  
MZ6  
MZ75  
MZ95  
MZ125  
MZ16/MZ16 A  
Achromat  
Achromat 1×  
10 411 589  
10 422 562  
10 447 081  
10 473 832  
10 445 201  
10 422 563  
10 422 564  
10 447 148  
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
Achromat 1.5×  
Achromat 2×  
Achromat 0.8×  
Achromat 0.63×  
Achromat 0.5×  
Achromat 0.32×  
Ergo objective 0.4× – 0.63×  
Planachromatic  
Planachromatic 1×  
10 446 275  
10 445 819  
10 446 157  
10 447 075  
C
C
C
C
Planachromatic 1× MZ125/MZ16  
Planachromatic 0.5× MZ125/MZ16  
Planachromatic 0.8×  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
C*M  
C*M  
C*M  
C
C
C
C
C
C
Planapochromatic  
Planapochromatic  
1× MZ125/MZ16  
10 447 157  
10 447 050  
10 447 051  
10 447 101  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
CA (10 446 172) M CA (10 446 172) M CA (10 446 172) M  
C*M  
C*M  
C*M  
C*M  
C
C
C
C
C
C
C
C
Planapochromatic  
1.6× MZ125/MZ16  
Planapochromatic  
0.63× MZ125/MZ16  
Planapochromatic  
2× MZ125/MZ16  
Accessories  
Coaxial incident light  
10 446 180  
10 445 618  
C
C
C
C
O (10 446 300)  
C
O (10 446 300)  
C
C
C
C
C
Microscope carrier AX  
MS5, MZ6, MZ75, MZ95  
Microscope carrier AX  
MZ125, MZ16, MZ16 A  
10 447 062  
10 445 198  
10 445 156  
10 447 107  
CA  
C
CA  
C
CA  
C
Near vertical illuminator  
CA 2× (10 446 300)  
CA 2× (10 446 300)  
(C)  
CA (10 446 300)  
CA (10 446 300)  
(C)  
CA (10 446 300)  
+ (10 446 393)  
CA (10 446 300)  
+ (10 446 393)  
Attachment for vertical and  
oblique observation  
C
C
CA (10 446 300)  
+ (10 446 393)  
CA (10 446 300)  
+ (10 446 393)  
Objective turret for 2  
×
and 1  
×
(C)  
(C)  
(C)  
C
planapo objectives  
C
Compatible  
CA Compatible if used with intermediate ring (order separately)  
0
Intermediate ring is recommended  
M
*
Magnification increased by factor 1.25× or higher  
Remove intermediate ring (10 446 393); already included with MZ95.  
(C) Large fields of view at low magnifications are not fully illuminated.  
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33  
Microscope carrier  
Microscope carrier The microscope carrier can be attached to the focusing drive in  
drive housing 2 positions:  
• For short working distances and for  
flat objects, use Position 1.  
Use this position also in conjunction  
with the attachment for vertical and  
oblique observation (see detailed in-  
structions).  
• Use Position 2 for long working  
distances and for bulky objects.  
̈ Use an Allen screw to secure the micro-  
scope carrier in the position required.  
A connection socket for a 4 mm  
grounding cable is provided on the  
microscope carrier.  
Microscope carrier AX • Different models are available for MS5,  
MZ6, MZ75, MZ95 and for MZ125, MZ16  
and MZ16 A (see p. 18).  
• The microscope carrier AX can only be  
fitted in position 1 to the focusing drive.  
Optics carrier  
Optics carrier ̈ Gently lower the optics carrier  
microscope carrier  
into the microscope carrier.  
̈ Use the screw to secure the optics  
carrier in the position required.  
Only for MZ75:  
Apply the adhesive ̈ Remove the screws.  
scale of ̈ Remove the rotary knobs.  
magnifications ̈ Apply the adhesive scales.  
̈ Replace the rotary knobs.  
34  
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Binocular tube  
̈ Release the clamping screw.  
• If you have an accessory such as an  
ergo module (page 14), a coaxial illumi-  
nator (page 47) or a video/phototube  
(page 52), fit it now to the optics carrier.  
Tighten the clamping screw.  
̈ Tilt the binocular tube and insert the  
edge of its dovetail ring beneath the  
two lugs on the optics carrier.  
̈ Move the binocular tube from side to  
side until the locating screw on the  
optics carrier engages the guide.  
̈ Retighten the clamping screw.  
Optical accessories  
Eyepiece ̈ Insert a graticule, if necessary (page 56).  
̈ Release the clamping screws on the eye-  
pieces.  
̈ Insert the eyepieces fully into the eye-  
piece tubes. Check that they have seated  
correctly.  
̈ Tighten the clamping screw.  
• You can extend the overall magnification  
range by using 10×, 16×, 25× and 40×  
wide-eyepieces for persons wearing  
glasses.  
Objective Hold the objectives during assembly and disassembly to pre-  
vent them from falling on the stage plate. This applies particu-  
larly to the 2× planapochromatic objective, which is very heavy.  
Remove the specimens from the stage plate.  
̈ Screw on the selected objective counter-  
clockwise.  
• Some instrument configurations require spacer rings in order  
to secure the objectives (page 32). These rings are fitted and  
removed using the tool provided.  
• The Leica MZ95 is supplied with a spacer ring for the achro-  
matic objectives and for the 1× planachromatic objective.  
When the spacer ring has been removed by applying the tool  
provided, the larger-diameter planachromatic and planapo-  
chromatic objectives for the MZ125/MZ16 can be used.  
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Objective nosepiece  
• Suggested for Leica MZ16 and MZ16 A  
Please note: When using the objective nosepiece together with MS5, MZ6,  
MZ75, MZ95 or MZ125 large fields of view at low magnifications are not fully  
illuminated.  
– Hold objectives during assembly and disassembly to prevent them from  
falling on the stage plate. This applies particularly to the 2× planapochro-  
matic objective, which is very heavy. Remove the specimens from the  
stage plate.  
– The adjustable ring nut with the 1× planapochromatic objective is fastened  
with 2 Allen screws after the adjustment. Loosen the Allen screws if you  
need to remove the objective.  
– Attach the limit stop to the stand column. It prevents the 2× objective from  
hitting the limit stop when the objective nosepiece is rotating, if the work-  
ing distance was changed ×while working with the 1 objective.  
Caution: Carefully rotate the objective nosepiece and take care not to  
pinch your fingers between column and objective or objective nosepiece.  
MZ16 A: When changing to objective 1× or 2×, press the OPT + SEL function  
keys simultaneously. The display shows the current total magnification, and  
in REF mode it shows the current measurement with objective 1× or 2×.  
A 1 or 2 in front of the value indicates the selected objective.  
Preparation Remove the securing strap from the objective nosepiece:  
̈ Remove both screws (at the bottom).  
Leica MZ16 and MZ16 A are delivered with a threaded adapter for objectives.  
Remove the adapter to use the objective nosepiece.  
̈ Invert the optics carrier.  
̈ Loosen 3 screws and remove the adapter.  
Assembling The objective nosepiece features two threads:  
1× planapochromatic – a fixed thread for the 2× planapochromatic objective  
– a thread with 2 Allen screws for the 1× planapochromatic objective with  
adjustable ring nut. The adjustable ring nut is supplied separately and used  
to adjust the parfocality between both objectives.  
Invert the objective nosepiece to simplify the assembly of the objectives.  
̈ Screw on the adjustable ring nut on the thread of the 1× planapochromatic  
objective until the limit stop and tighten it with the enclosed wrench.  
̈ Screw in the 1× planapochromatic objective with the ring nut in clockwise  
direction on the side with the 2 Allen screws.  
• The fine-pitch thread on the ring nut consists of 24 threads.  
You should expect at least 40 turns.  
The adjustable ring nut must never be set higher than the top surface of the  
nosepiece plate to prevent the nosepiece from locking while rotating.  
36  
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Assembling ̈ Carefully turn and tighten the 2× planapochromatic objective clockwise to  
2× planapochromatic  
the limit stop without applying excessive force.  
Assembling ̈ Raise the focusing drive with the drive heads to  
objective nosepiece  
provide sufficient space for the 2× planapochro-  
matic objective.  
̈ Fit the objective nosepiece like a microscope car-  
rier with the Allen screw (see page 34, position 1).  
̈ Insert the optics carrier in the objective nosepiece.  
̈ Retighten the clamping screw.  
̈ Attach the limit stop to the column of the stand so  
that the working distance beneath the 2× objective  
will always measure approx. 15 mm.  
Adjusting parfocal • Fit and adjust the 1× and 2× planapochromatic objectives as described.  
The image then remains parfocal in the range of < 0.05 mm, and refocusing  
after changing the objective is no longer required.  
• Use a very fine, flat object (e.g. an object micrometer) for the adjustment,  
which allows maximum focusing even at highest resolution.  
̈ Screw the 2× planapochromatic objective into the beam path.  
̈ Adjusting the diopter settings and parfocality (see p. 20).  
̈ Screw the 1× planapochromatic objective into the  
beam path.  
̈ Observe the same object with smallest magnifica-  
tion without changing the settings made with the  
2× planapochromatic objective.  
̈ Turn the 1× planapochromatic objective counter-  
clockwise until the object can be seen in focus  
at the smallest magnification.  
̈ Slowly zoom to the highest magnification. At  
the same time correct the focus by turning the  
1× planapochromatic objective.  
• Verify that the object is in focus at highest magnification with the  
2× planapochromatic as well as the 1× planapochromatic objective  
without refocusing. Otherwise turn the objective at the ring nut until  
the object is focused.  
̈ Fasten the ring nut with both screws.  
Do not overtighten both screws.  
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Illuminators  
Transformers  
Follow the safety instructions given on page 7.  
Leica transformers may only be opened by authorized service  
personnel, and the power cable must be disconnected before-  
hand. The transformers are not to be used outdoors.  
Regulating transformer  
The regulating transformer 5.3 to 7.5 V/40 VA is used to operate  
the 6 V/10 W and 6 V/20 W lamps.  
1 Connections for two lamps (maximum combined  
loading 40 W)  
2 Connection for power cable  
3 Fuse holder 2x T 1A L 250 VA  
4 Power switch  
I
0
on  
off  
5 ON display: Instrument is switched on.  
6 Regulating knob  
7 6 V display: The standard voltage has been reached.  
Technical data see page 74  
2
3
7
5
1
4
6
1
Starting up Switching the input voltage is not necessary.  
The adjustment is carried out automatically by means of the  
switching controller in the regulating transformer.  
̈ Connect the power cable to the socket of the base and to  
the power source.  
̈ Connect the lamp. The sockets accept two lamps with  
a maximum combined loading of 40 W.  
38  
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Switching You can prolong the life of your bulb if you:  
on and off  
̈ First switch off the lamp by turning the  
rotary knob into the black range  
̈ and only then switch the transformer  
on or off.  
̈ Turn on the power switch.  
The diode is illuminated.  
̈ Use the rotary knob to adjust the  
brightness.  
Above 6 V the diode is illuminated.  
Changing fuses Fuses 1.0A, 5×20 (stock no. 167 651)  
̈ Lamp off.  
̈ Power switch off.  
̈ Use a flat-bladed screwdriver to press on  
the slots in the fuse holders (3) and turn  
the fuse holders to the left.  
Pull out the fuse holder.  
̈ Fit a new fuse and replace the fuse  
holder.  
In the event of ̈ Lamp off.  
overload ̈ Power switch off.  
̈ Wait a few seconds.  
̈ Power switch on.  
̈ Lamp on.  
Step transformer  
Follow the safety instructions given on page 7.  
• You can attach a 6 V/10 W lamp to the  
step transformer.  
̈ Set the voltage selector on the rear  
side to 115 V or 230 V.  
̈ Attach the power cable to the power  
supply.  
̈ Connect the lamp.  
̈ Adjust the brightness with the three-step switch (4/5/6 V).  
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6 V/10 W incident lamp  
• The 6 V/10 W lamp is an inclined incident source for  
illuminating spatial objects  
• By using two lamps, you can soften or eliminate shadows.  
• At the nominal voltage (6 V), the color temperature is 2700 K.  
Assembly ̈ Release the clamping screw.  
̈ Unfold the lampholder arm.  
̈ Retighten the clamping screw.  
Lampholder ̈ Use the screw to secure the clamp to  
clamp for side-faced  
columns  
the side-faced column, either above or  
below the focusing drive.  
̈ Use the connector to attach the lamp-  
holder arm to the clamp, on either the  
right or the left.  
Lampholder ̈ Fit the 25 mm adapter to the cast foot.  
cast foot ̈ Using the connector, attach the lampholder to the adapter.  
Lampholder ̈ Remove the objective.  
objective ̈ Slide the adapter over the objective.  
̈ Using the connector, attach the lampholder to the adapter.  
Lampholder ̈ Unscrew a connector from the lampholder.  
inclinable focusing ̈ Release the clamping screw.  
drive ̈ Using the thread, secure the lampholder to the focusing drive.  
̈ Retighten the clamping screw.  
Lamp housing ̈ Attach the lamp to the connector on  
lampholder arm  
the lampholder arm.  
̈ Fit the filter holder, complete with KG1  
heat-absorbing filter, to one of the pegs.  
• A total of 4 filter holders with 50 mm  
filters can be attached.  
40  
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Operating the lamp  
Observe the safety notes on page 7.  
Observe the notes with respect to the use of the trans-  
former on page 38.  
̈ Connect the cable between the lamp  
housing and the transformer (page 38).  
̈ Switch on the transformer and use the  
rotary knob to adjust the brightness  
(page 39).  
̈ Hold the lamp housing and release  
the clamping screw.  
̈ Align the light spot with the object.  
̈ Retighten the clamping screw.  
̈ Swing in the heat-absorbing filter.  
̈ Enlarge or reduce the size of the light  
spot by turning the socket of the bulb.  
Changing  
the bulb  
Disconnect the lamp cable from the transformer.  
Never touch the bulb when it is hot.  
You may sustain burns.  
• You can separate the lamp housing and the bulb socket. Line  
up the arrow head on the mount with its shaft on the housing.  
• Do not touch the 6 V/10 W halogen bulb because finger  
marks can cause it to crack when heated. Remove finger  
marks with a cloth dipped in alcohol.  
̈ Turn the socket to the separation  
position.  
̈ Pull the socket forcibly out of the housing.  
̈ Hold the new bulb in a cloth and push it  
into position.  
̈ Replace the socket in the housing. The  
socket clicks into position.  
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41  
6 V/20 W incident lamp  
• The 6 V/20 W lamp is an inclined incident source for  
illuminating spatial objects.  
• By using two lamps, you can soften or eliminate shadows.  
• At the nominal voltage (6 V), the color temperature is 3200 K,  
ideal for photography. Set the regulating transformer to this  
nominal voltage (see page 38).  
̈ Release the clamping screw.  
̈ Unfold the lampholder arm.  
Assembly  
̈ Secure the lampholder to the lamp.  
̈ Use the screw to secure the clamp to  
the side-faced column, either above or  
below the focusing drive.  
̈ Using a fork wrench and the thread con-  
nector, attach the lampholder arm to the  
clamp, on either the right or the left.  
̈ Position the lamp.  
̈ Retighten the clamping screw.  
• The thread connector also enables the lampholder  
to be connected to the cast foot.  
• A total of 4 filter holders with 32 mm filters can  
be attached.  
A heat-absorbing filter is built in.  
Diffusing and  
other filters  
̈ Push the filter holder, complete with  
diffusing filter, onto one of the pegs,  
and swing it in.  
42  
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Operating the lamp  
Observe the safety notes on page 7.  
Observe the notes with respect to the use of the  
transformer on page 38.  
̈ Connect the cable between the lamp housing and the trans-  
former (page 38).  
̈ Switch on the transformer and use the  
rotary knob to adjust the brightness.  
Illuminating the object ̈ Push the condenser lens forwards  
or backwards until the slider engages.  
̈ Hold the lamp housing and release  
the clamping screw.  
̈ Align the light spot with the object.  
̈ Retighten the clamping screw.  
̈ Adjust the size of the light spot by  
moving the condenser lens forward  
or back.  
̈ If necessary, swing in a filter (page 42).  
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Changing the bulb  
Disconnect the lamp cable from the transformer.  
Never touch the bulb when it is hot.  
You may sustain burns.  
Do not touch the 6 V/20 W halogen bulb directly with your fin-  
gers, because finger marks can cause it to crack when heated.  
Remove finger marks immediately with a cloth dipped in pure  
alcohol.  
̈ Press the spring clips together and  
pull off the rear part of the housing.  
̈ Hold the new bulb in a cloth and push  
it into position.  
̈ Press the spring clips together and  
close the housing again.  
̈ Center the bulb.  
Centering the bulb The 6 V/20 W bulb is a powerful source of illumination. The  
more carefully you set it up, the more uniform the lighting will  
be. For this reason, recenter a new bulb after fitting it.  
̈ Switch on the lamp.  
̈ Swing out the diffusing filter.  
̈ Pull the condenser lens fully out.  
̈ Hold the lamp and release the  
clamping screw.  
̈ Point the lamp vertically downwards  
at a white sheet of paper so that two  
filament images are seen.  
̈ Retighten the clamping screw.  
̈ Using the slotted screw, bring the  
two filament images into focus.  
̈ Displace the filament images so that  
they are opposite to one another, but  
in contact.  
̈ Swing back the diffusing filter.  
44  
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Leica L2 cold-light source  
The antistatic Leica L2 cold-light source is powerful, small,  
compact, inexpensive and suitable for simple observation tasks  
with the Leica MS5 and MZ6 stereo microscopes. In addition to  
one or two-arm light guides, corresponding accessories are al-  
so available for the coaxial, vertical and transmitted-light illumi-  
nation methods. The Leica L2 can be directly connected on the  
stand.  
The light guides can be mounted with arms in the threads on  
the microscope carrier. For instructions on mounting the clamp  
for fiber-optic light guides, see page 46.  
For detailed information, refer to the separate user manual  
Leica L2 M2-288-0.  
When in use, the Leica L2 fiber-optic light source must always  
be locked into an adapter to ensure stability.  
̈ Secure the clamp to the 300 mm  
focusing drive by tightening the screw.  
̈ Using the mounting rail, slide the fiber-  
optic light source into the adapter fork  
until it engages.  
Adapter ➜  
300 mm focusing drive  
When mounted on the baseplate, the light  
source can be used as a stand-alone unit.  
Adapter ➜  
baseplate  
̈ Using the mounting rail, slide the fiber-  
optic light source into the adapter fork  
until it engages.  
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Cold light source Leica CLS series  
The Leica CLS series is a high-performance line for high light  
intensity within the smallest possible space and flicker-free  
white light with the lowest possible thermal effect on the ob-  
jects. The comprehensive line of accessories enables unlimited  
use. The CLS series is produced of antistatic material.  
When using the ring lamp (76 mm) on the planachromatic  
objective 0.8× (80 mm), a special adapter (10 447 078) is  
required.  
For a detailed information, refer to the user manual Leica CLS.  
Fiber-optic • The clamp of the fiber-optic light guide cannot be  
light guide  
attached to the microscope carrier AX.  
̈ Turn the clamping screw out of the clamp.  
̈ Hold the clamp in the opening of the  
microscope carrier.  
̈ Push the clamping screw through the  
holes in the microscope carrier and in  
the broader part of the clamp, and screw  
it finger-tight to the narrow part of the  
clamp.  
̈ Insert the fiber-optic light guide into the clamp.  
̈ Switch on the power unit.  
̈ Align the light spot with the object.  
̈ Retighten the clamping screw.  
Leica LED illumination  
The Leica LED1000 (Laser Emitting Diode) illumination is avail-  
able with ring illuminator and/or spot and suitable for routine  
tasks with the Leica MS5, MZ6, MZ75, MZ95 stereomicroscopes.  
LEDs, which do not develop any heat, are used as illuminators.  
The color temperature measures 5000 K (daylight).  
For details see the separate instructions for use for the LED  
illumination.  
46  
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Supply (line) lamp 25W  
Follow the safety instructions given on  
page 7.  
̈ Fit the lamp holder and lamp as  
described below (incident-light  
lamp 6 V/10 W).  
̈ Connect the 25 W supply (line)  
lamp to the power supply.  
̈ Switch on the supply (line) lamp.  
̈ Align the light spot with the object  
as described.  
Coaxial illuminator  
• This illuminator is for observing and photographing flat,  
highly-reflecting objects such as wafers, integrated circuits  
and polished metal sections.  
• The coaxial incident light housing fits like an accessory tube  
between the optics carrier and the binocular tube, or between  
the optics carrier and the phototube (page 50).  
• For the MZ75 and MZ95, fit the objective  
preferably to the spacer ring (10 446 300),  
page 32. This will completely illuminate  
the largest field of view.  
• A quarter-wave plate is required when the microscope  
carrier AX is used in the vertical position.  
̈ Using the clamping screw, secure the quarter-wave plate to  
the objective mount.  
̈ Using the knurled ring, turn the quarter-wave plate until the  
required effect is obtained.  
Please observe the notes on the use of the aperture in the  
AX microscope carrier (p. 19).  
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Near-vertical illuminator  
• The near-vertical (approx. 5°) light beam illuminates depres-  
sions and cavities.  
• Shadowing by tools is eliminated.  
• This illuminator can only be used with the achromatic objec-  
tives having focal lengths of 100, 150, 175 and 200 mm.  
• Spacer rings are required for the Leica MZ75, MZ95, MZ125  
to secure the near-vertical illuminator to the optics carrier  
(page 32).  
̈ Unscrew the objective.  
̈ Release the clamping screw and remove the adapter ring  
from the incident-light housing for near-vertical light.  
For MS5, MZ6: ̈ Screw the adapter ring onto the  
microscope carrier.  
̈ Secure the incident-light housing  
to the adapter ring.  
For Leica MZ75, ̈ First screw the spacer ring(s) (page 32)  
MZ95, MZ125:  
onto the microscope carrier, then screw  
the adapter ring to the spacer ring.  
̈ Secure the incident-light housing to the  
adapter ring.  
̈ Screw the achromatic objective (see  
above) into position.  
̈ Release the left or right clamping screw  
and change the cover plate accordingly.  
48  
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̈ Attach the fiber-optic light guide on  
either the left or the right.  
To switch on the cold-light illuminator,  
refer to the user manual provided with it.  
No light?  
̈ Turn the rotary knob.  
• A movable prism directs the light from  
the left or right outlet onto the object.  
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Accessories  
Tubes  
Assembly • All accessory tubes which fit between the optics carrier and  
the binocular tube are fitted in the same manner.  
• In the dovetail ring of the optics carrier is a locating screw  
which must engage the notch in the accessory (page 35).  
This is best accomplished by moving the accessory slightly  
from side to side.  
̈ Release the clamping screw.  
̈ Insert the accessory tube into the dove-  
tail ring in the same manner as the binoc-  
ular tube (page 35) and move it slightly  
from side to side until the locating screw  
engages the notch.  
̈ Retighten the clamping screw.  
̈ Fit the binocular tube on the accessory  
tube in the same manner.  
Double-iris diaphragm  
A double-iris diaphragm is built into the MZ16 and MZ16 A. It is  
operated by means of a knurled ring. A separate double-iris  
diaphragm is available as accessory for the other instruments.  
The double-iris diaphragm for MS5–MZ125  
can be inserted between optics carrier and  
binocular tube or video/phototube.  
Set the lever to OPEN: The double-iris  
diaphragm (aperture diaphragm) is open.  
The depth of field increases as the lever  
is moved from OPEN to 1. Remember,  
however, that  
– brightness decreases, so exposure times become longer  
– resolution deteriorates.  
50  
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Drawing tube  
• The drawing tube is for both left-handers and right-handers.  
Fit the mirror on the left-hand or right-hand side of the optics  
carrier.  
̈ Fit the drawing tube between optics car-  
rier and binocular tube as described on  
page 50.  
̈ Place a sheet of paper beneath the  
mirror.  
̈ Use a table lamp to illuminate  
the paper.  
Drawing ̈ Move the lever to the circle.  
̈ Look into the binocular tube, position  
the object, and focus on it.  
̈ Move the lever to the black spot.  
̈ Look into the binocular tube and balance  
the brightness so that the object, the  
drawing surface and the pencil are all  
seen together.  
• In general, the paper needs to be illuminated strongly, but the  
object only weakly.  
̈ Move the lens mount of the drawing tube forwards or back-  
wards until the drawing surface is sharply imaged.  
̈ Now you can draw the feature.  
• If you place a graduated scale beneath the object and draw  
its intervals as well, you can easily determine the image  
scale on the paper.  
Discussion tube  
At the discussion tube two observers see the same erect,  
laterally-correct, three-dimensional image.  
The assembly and use of the discussion tube are described in  
the separate user manual M2-263-0.  
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Video/phototubes  
1”, 2/3”, 1/2”, 1/3”  
C-Mount, CCD cameras  
Leica DC cameras  
Leica  
MPS30 / MPS60  
photoautomats  
B-Mount  
3-chip 2/3”  
B-Mount  
3-chip 1/2”  
C-Mount  
1”  
SLR  
camera housing  
CCD camera CCD camera CCD camera  
11 543 706  
11 543 703  
11 543 702  
Adapter  
SLR  
11 541 038  
0.55x -1.1x  
10 446 120  
8x  
10 445 304  
10x  
10 162 226  
10 445 305  
16x  
10 445 541  
10 404 207  
10 446 261  
2/3”, 0.63x  
10 446 307  
0.8x  
11 541 006  
1”, 1x  
10 446 175  
10 445 929  
1/2”, 0.5x  
10 445 932  
11 541 007 10 445 928  
2/3”, 0.63x 1/3”, 0.32x  
11 541 016  
1/2”, 0.5x  
10 445 930  
1x  
10 446 121 8x  
10 446 131 10x  
10 446 132 16x  
HD F 10 446 308  
HD V 10 446 309  
10 445 924 50%  
10 446 229 100%  
Leica MZ16, MZ16 A  
HD50  
10 446 197  
H
A
10 445 931  
10 445 925  
Leica MS5, MZ6, MZ75, MZ95,  
MZ125, MZ FLIII  
10 445 927  
52  
Leica M Stereomicroscopes – Accessories  
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• Video/phototubes are designed to support modular  
photomicrography systems, and Leica digital, video, film  
or SLR cameras.  
• The modular photomicrographic systems and the Leica digi-  
tal cameras are detailed in a separate user manual, along  
with instructions for their assembly and use.  
• When you are not taking photographs, engage the observa-  
tion beam path.  
• Accessories such as the coaxial incident illuminator always  
fit between the stereomicroscope and the phototube.  
Video/phototube A  
This tube does not permit stereoscopic observation. The image  
is studied either in the camera viewfinder, or down the focusing  
telescope of the camera. In the video/photo beam path, 100%  
light is available.  
Trinocular video/phototube 50%  
̈ Fit the trinocular video/phototube on  
the optics carrier in place of the  
binocular observation tube (page 35).  
For examples on camera assembly  
see page 54.  
Partition of light • 50% to both eyepieces  
• 50% in the video/photo beam path  
Stop Before taking the photograph, close off  
the right eyepiece to keep out stray light.  
̈ Move lever to the left.  
Leica M Stereomicroscopes – Accessories  
53  
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Trinocular video/phototube 100%  
Fitting: See "Trinocular video/phototube 50%".  
Switchover ̈ Observation: Push lever in.  
̈ Photography: Pull out lever to the left.  
Light partition • 100% in both eyepieces.  
setting "VIS"  
Light partition • 100% in the left eyepiece  
setting "PHOT" • 100% in the video/photo beam path.  
Camera setup, As shown in the setup diagram on page 52, there are various  
variants possibilities for adapting an MPS, video, digital or SLR camera.  
Example:  
With this equipment you can, for example, attach an MPS  
camera (see detailed description in the MPS user manual), an  
SLR camera, or even a digital or video camera.  
̈ Fit the 1× video/photo objective.  
Example:  
With this equipment you can, for example, attach a digital  
or video camera.  
̈ Fit the video objective (2) on a Leica  
DC digital camera (3) with the C-mount  
connection.  
̈ Connect the video objective with the  
mounted Leica DC digital camera on  
the right output of the video/phototube.  
̈ Tighten the coupling ring (1).  
54  
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Video/phototube HD50  
̈ Use the video/phototube HD50 between  
the optics carrier and the binocular tube  
(page 50).  
Switchover ̈ Observation: Push lever in.  
̈ Documentation: Pull lever out.  
Light partition • 50% to both eyepieces  
for "observation" • 50% in the left-hand video/photo beam path  
• 50% in the right-hand video/photo beam path  
Light partition • 100% to left video/photo beam path  
for "documentation" • 50% to right eyepiece  
• 50% in the right-hand video/photo beam path  
Camera setup, variants As shown in the setup diagram on page 52, there are various  
possibilities for adapting an MPS, video, digital or SLR camera.  
Example:  
̈ Fit the video/photo objective  
H on the left-hand side.  
With this equipment you can, for example, attach an MPS  
camera (see detailed description in the MPS user manual), an  
SLR camera, or even a digital or video camera.  
Example:  
With this equipment you can, for example, attach a digital  
or video camera.  
̈ Fit the video objective (2) on a  
Leica DC digital camera (3) with  
the C-mount connection.  
̈ Connect the video objective  
with the mounted Leica DC digital  
camera on the right output of the  
video/phototube.  
̈ Tighten the coupling ring (1).  
Leica M Stereomicroscopes – Accessories  
55  
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Attachment for vertical and oblique observation  
• The attachment for vertical and oblique observation enables  
spatial objects to be observed from above, and also from all  
around at an angle of 45°.  
• The microscope carrier must be fitted in the lower  
position 1 (page 34).  
• For MS5, MZ6: Fit the 1× achromatic objective by screwing  
its adapter ring directly into the optics carrier.  
• Spacer rings are required for the Leica  
MZ75, MZ95, MZ125 and MZ16 to secure  
the attachment for vertical and oblique  
observation to the optics carrier (page 32).  
• Only usable with 1× achromatic objec-  
tive.  
A detailed description can be found in the  
user manual M2-266.  
Graticules  
• The graticules for length measurements and numbering are  
fitted in mounts and can be inserted into the eyepieces.  
• A special measuring graticule is available for the Leica MZ16  
A (see the user manual M2-116).  
• A high-precision object micrometer with 50-mm scale,  
0.1 mm and 0.01 mm graduation, allows for calibration  
corresponding to the selected magnification.  
• Preferably measure with the microscope carrier AX in  
vertical position. The measurements are more accurate with-  
out the convergence angle in the stereoscopic image.  
• Graticules show boundary lines for all film formats.  
Inserting the ̈ Unscrew the insert at the knurled ring.  
graticule ̈ Clamp the graticule onto the insert with  
a little bit of pressure. Check for correct  
seating!  
̈ Tighten the insert with graticule.  
̈ Insert the eyepiece.  
̈ Look into the eyepiece: Align the gratic-  
ule by turning the eyepiece in the tube  
and tighten the clamping screw.  
A detailed description can be found in the  
user manual M2-275.  
56  
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Stages  
The 120 mm stages can be used in those incident and trans-  
mitted-light stands which have a 120 mm port.  
Gliding stage The gliding stage can be moved in all directions and  
rotated completely.  
̈ Place the 120 mm gliding stage in  
the 120 mm port and secure it with  
the eccentric lever.  
̈ Insert the 120 mm glass stage plate or  
the black/white stage plate.  
Cup stage • The cup stage can be inclined as required.  
• You can secure objects to its rubberized upper  
surface with needles.  
• The movable holder accepts petri dishes.  
̈ Place the 120 mm  
support ring in the  
120 mm port.  
̈ Place the cup stage on it.  
Thermo stages The Leica MATS thermocontrol system is a heating system for  
microscopes and stereomicroscopes. Leica MATS allows the  
observation of temperature-sensitive specimens and living cells  
in biology, medicine and pharmaceutics under exact tempera-  
ture conditions.  
For details see M2-227-0 instructions for use.  
Polarization, transmitted light  
The analyzer, in combination with either the rotatable polariza-  
tion stage or the polarizer on glass stage plate, enables double-  
refracting materials to be studied. These can include organic  
crystals, mineral grains, thin sections of rock or bone, polymers,  
stressed glass, and liquid crystals.  
A detailed description can be found  
in the user manual M2-277.  
Leica M Stereomicroscopes – Accessories  
57  
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Notes  
Tips and hints: What to do if …  
The field of view is Remedy:  
partly shadowed.  
• Set the interpupillary distance correctly (page 15).  
• Locate the eyepoint (page 15).  
The image does not Remedy:  
stay in focus.  
• Fit the eyepieces correctly (page 35).  
• Adjust your dioptric settings exactly as described (page 20).  
The focusing drifts Remedy:  
downwards or can  
be moved only with  
difficulty.  
• Adjust the ease of movement (pages 16, 29, 31).  
If an electrical fault • Is the voltage selector set correctly (bright-field transmitted  
develops, first check light stand, page 24; transformer, page 38)?  
the following: • Is the power switch on?  
• Is the power cable attached correctly?  
• Are all connecting cables attached correctly?  
• Are the fuses in order?  
The image Remedy:  
is too dark.  
• Turn up the regulating knob high enough (bright-field  
transmitted light stand, page 24; transformer, page 38).  
• Open the double-iris diaphragm of the phototube.  
TV, photography  
The image Remedy:  
on the screen  
• Adjust the video camera / monitor system in accordance  
is too dark.  
with the directions of the manufacturer.  
Refer to the remedies listed above under "The image is too dark."  
Blurred Remedy:  
photographs.  
• Focus precisely (page 16).  
• Adjust your dioptric settings exactly as described (page 20).  
Color photographs Remedy:  
have a yellow cast.  
• Use artificial-light film.  
• Turn the regulating knob of the transformer clockwise to the  
position of maximum voltage (bright field transmitted-light  
stand, page 24; transformer, page 38).  
58  
Leica M Stereomicroscopes – Notes  
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Care, maintenance  
In this section We will show you how to look after your microscope and how  
to clean it.  
We guarantee You are working with a high-precision, high-performance  
the quality. instrument.  
Accordingly, we guarantee the quality of each of our instru-  
ments. The guarantee covers all faults in materials and manu-  
facture. It does not, however, cover damage resulting from  
careless or improper handling.  
Please handle your valuable optical instrument with care. It will  
then fulfill its function with the same precision for decades. Our  
instruments are known for that!  
If, contrary to expectations, your stereomi-  
croscope develops a fault, please call in a  
specialist – contact your local Leica agent  
or Leica Microsystems (Switzerland) Ltd,  
CH-9435 Heerbrugg.  
Protect your • From moisture, vapors, acids, alkalis and  
instruments  
corrosive substances, and keep chemicals  
away from the instruments.  
• From improper handling.  
Never dismantle optical systems or  
mechanical parts unless instructions for  
doing so are given in the user manual.  
• From oil and grease.  
Do not grease guide surfaces or  
mechanical parts.  
Leica M Stereomicroscopes – Notes  
59  
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Cleaning  
Dust and dirt will affect the quality of your results.  
Therefore: ̈ Put a dust cover over the instrument  
during breaks in work.  
̈ Use dust caps to protect  
tube openings, tubes without  
eyepieces, and eyepieces.  
̈ Remove dust with a pneumatic  
rubber bulb or with a soft brush.  
̈ Clean objectives and eyepieces  
with special optic cleaning  
cloths and with pure alcohol.  
̈ When accessories are not being  
used, protect them against dust.  
Cleaning polymer Some components are made of polymer, or are polymer-coated.  
components They are, therefore, pleasant and convenient to handle. The use  
of unsuitable cleaning agents and techniques can damage  
polymers, so always observe the following rules:  
Never clean • Never clean them in an ultrasonic bath. The polymer can  
polymers this way  
become brittle and later break.  
• Never use corrosive cleaning agents or ones containing  
acetone.  
• Never use solvents for cleaning (except for ethanol and  
isopropanol).  
You can clean • With warm soapy water, wiping down afterwards  
polymers harmlessly  
with distilled water.  
• With ethanol (industrial alcohol) and isopropanol.  
When cleaning with ethanol or isopropanol, adhere  
to the appropriate safety regulations.  
60  
Leica M Stereomicroscopes – Notes  
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Calculation  
Total magnification  
and field of view  
diameter  
M
M
z
Magnification of objective  
O
E
Magnification of eyepiece  
Magnification changer position  
Tube factor, e.g. 1.5× for coaxial incident light,  
q
TM  
45° ErgoTube 1.6×  
r
Factor 1.25× if the planachromatic and planapochro-  
matic objectives of the MZ125/MZ16 are used on the  
MS5, MZ6, MZ75 or MZ95  
N
Field number of eyepiece. The field numbers are printed  
on the eyepieces:  
10× = 21, 16× = 14, 25× = 9.5, 40× = 6.  
FOV  
Example  
M
M
z
Eyepiece 1×  
O
E
25×/9.5 eyepiece  
Zoom position 4  
Tube factor 1.5× (because of coaxial illuminator).  
Factor 1.25×  
q
r
Magnification down binocular tube:  
1 × 25 × 4 × 1.5 × 1.25 = 187.5 ×  
M
= M × M × z × q × r  
O
TOT VIS  
E
Diameter of field of view:  
N
FOV  
9.5  
OF :  
= 1.3 mm  
M
× z × q × r  
O
1 × 4 × 1.5 × 1.25  
Leica M Stereomicroscopes – Notes  
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61  
Optical data of Leica MS5, MZ6  
1× Plan  
1.6× Planapo* 0.63× Planapo* 0.5× Plan*  
1× Planapo*  
0.32× Achromat 0.5× Achromat 1.5× Achromat  
1× Achromat  
0.8× Plan*  
2× Achromat  
0.8× Achromat 0.63× Achromat  
Objectives  
Working distance (mm)  
81 Plan  
55 Planapo  
19 Planapo  
97 Planapo  
135 Plan  
297 Achromat 187 Achromat 49 Achromat  
89 Achromat  
112 Plan  
27 Achromat  
112 Achromat 149 Achromat  
Eyepieces  
0.63  
0.8  
1
6.3  
8
33.3  
26.3  
21  
7.9  
10  
26.6  
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
16.7  
5
42  
3.9  
5
53.8  
42  
2
105  
84  
3.2  
4
65.6  
52.5  
42  
9.4  
11.9  
14.9  
18.7  
23.9  
29.9  
37.3  
47.8  
59.7  
22.3  
17.6  
14.1  
11.2  
8.8  
21  
13.1  
10.5  
8.4  
6.4  
8
32.8  
26.3  
21  
2.5  
3.1  
3.9  
5
10  
12.5  
15.6  
20  
16.8  
13.5  
10.5  
8.4  
6.3  
7.8  
33.3  
26.9  
21  
67.7  
5
1.25  
1.6  
2
12.5  
16  
16.8  
13.1  
10.5  
8.4  
10  
53.8  
42  
6.3  
8
33.3  
26.3  
21  
10×/21B  
16×/14B  
25×/9.5B  
6.6  
12.8  
16  
16.4  
13.1  
10.5  
8.2  
10  
20  
25  
5.3  
12.5  
15.6  
20  
16.8  
13.5  
10.5  
8.4  
6.3  
7.8  
33.3  
26.9  
21  
10  
7
2.5  
3.2  
4
25  
31.3  
40  
6.7  
4.2  
20  
12.5  
16  
16.8  
13.1  
10.5  
5.6  
32  
6.6  
5.3  
3.3  
25.6  
32  
10  
4.4  
40  
5.3  
50  
4.2  
2.6  
6.6  
25  
12.5  
16.8  
20  
3.5  
0.63  
0.8  
1
10.1  
12.8  
16  
22.2  
17.5  
14  
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
17.8  
14  
20.2  
25.6  
32  
11.1  
8.8  
7
8.1  
10.2  
12.8  
16  
27.7  
22  
6.3  
8
35.6  
28  
3.2  
4
70  
5
44.8  
35  
15  
14.9  
11.7  
9.4  
7.5  
5.9  
4.7  
3.8  
2.9  
2.3  
56  
6.4  
8
19.1  
23.9  
29.9  
38.2  
47.8  
59.7  
76.4  
95.5  
11.2  
9
17.5  
14  
10  
22.4  
17.9  
14  
5
44.8  
35.6  
28  
28  
1.25  
1.6  
2
20  
11.2  
8.8  
7
40  
5.6  
4.4  
3.5  
2.8  
2.2  
1.8  
12.5  
16  
6.3  
8
10  
22.4  
17.5  
14  
25.6  
32  
7
51.2  
64  
20.5  
25.6  
32  
10.9  
8.8  
7
12.8  
16  
5.6  
4.5  
3.5  
2.8  
20  
11.2  
9
10  
12.5  
16  
20  
22.4  
17.9  
14  
2.5  
3.2  
4
40  
5.6  
4.4  
3.5  
80  
25  
20  
11.2  
8.8  
7
51.2  
64  
102.4  
128  
41  
5.5  
4.4  
32  
7
25.6  
32  
51.2  
40  
5.6  
11.2  
0.63  
0.8  
1
15.8  
20  
15  
19.7  
25  
12.1  
9.5  
7.6  
6.1  
4.8  
3.8  
3
31.5  
40  
7.5  
5.9  
4.8  
3.8  
3
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
18.8  
14.8  
11.9  
9.5  
7.4  
5.9  
4.8  
3.7  
3
9.8  
12.5  
15.6  
19.5  
25  
24.2  
19  
4.9  
6.3  
48.5  
37.7  
30.4  
24.2  
19  
7.9  
10  
30.1  
23.8  
19  
23.5  
29.9  
37.3  
46.6  
59.7  
74.6  
93.3  
119.4  
149.3  
10.1  
7.9  
6.4  
5.1  
4
11.9  
9.5  
7.6  
5.9  
4.8  
3.8  
3
25  
31.3  
39.1  
50  
50  
15.2  
12.2  
9.5  
7.8  
12.5  
15.6  
20  
1.25  
1.6  
2
31.3  
40  
62.5  
80  
9.8  
15.2  
11.9  
9.5  
12.5  
15.6  
19.5  
25  
50  
62.5  
78.1  
100  
125  
160  
200  
2.4  
1.9  
1.5  
1.2  
31.3  
39.1  
50  
7.6  
15.2  
12.2  
9.5  
25  
3.2  
2.5  
2
2.5  
3.2  
4
62.5  
80  
6.1  
31.3  
40  
7.6  
100  
125  
2.4  
1.9  
4.8  
5.9  
100  
2.4  
62.5  
3.8  
31.3  
7.6  
50  
4.8  
1.6  
0.63  
0.8  
1
25.2  
32  
9.5  
7.5  
6
31.5  
7.6  
6
50.4  
64  
4.8  
3.8  
3
20.2  
25.6  
32  
11.9  
9.4  
7.5  
6
15.8  
20  
15.2  
12  
7.9  
10  
30.4  
24  
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
19  
15  
12  
9.6  
7.5  
6
37.6  
47.8  
6.4  
5
40  
40  
50  
4.8  
3.8  
3
80  
25  
9.6  
7.7  
6
12.5  
15.6  
20  
19.2  
15.4  
12  
59.7  
4
1.25  
1.6  
2
50  
4.8  
3.8  
3
62.5  
80  
100  
128  
160  
200  
256  
320  
2.4  
1.9  
1.5  
1.2  
0.9  
0.8  
40  
31.3  
40  
74.6  
3.2  
2.5  
2
40×/6B  
64  
51.2  
64  
4.7  
3.8  
3
95.5  
80  
100  
2.4  
1.9  
1.5  
1.2  
50  
4.8  
3.8  
3
25  
9.6  
7.7  
6
119.4  
149.3  
191  
2.5  
3.2  
4
100  
2.4  
1.9  
1.5  
125  
160  
200  
80  
62.5  
80  
31.3  
40  
4.8  
3.8  
3
1.6  
1.3  
1
128  
160  
102.4  
128  
2.3  
1.9  
100  
2.4  
50  
4.8  
238.8  
MS5: Positions 0.63, 1, 1.6, 2.5, 4  
* When using the planachromatic and planapochromatic objectives MZ125, the magnification is increased  
by the factor 1.25×.  
62  
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Ergo objective  
0.4×–0.63×  
Objectives  
Working distance (mm)  
63.5 mm  
153.5 mm  
Eyepieces  
0.63  
0.8  
1
4
52.5  
41.2  
32.8  
26.3  
20.6  
16.5  
13.2  
10.3  
8.2  
2.6  
3.3  
4
80.8  
63.6  
51.2  
41.2  
31.8  
25.6  
20.4  
15.9  
12.7  
5
6.4  
8
1.25  
1.6  
2
5
10×/21B  
16×/14B  
25×/9.5B  
10.2  
12.7  
15.9  
20.4  
25.5  
6.6  
8.2  
10.3  
13.2  
16.5  
2.5  
3.2  
4
0.63  
0.8  
1
6.4  
8.2  
35  
4
54.6  
42.3  
33.9  
27.3  
21.3  
17  
27.3  
22  
5.3  
10.2  
12.7  
16.3  
20.4  
25.5  
32.6  
40.8  
6.6  
1.25  
1.6  
2
17.6  
13.7  
11  
8.2  
10.5  
13.2  
16.5  
21  
2.5  
3.2  
4
8.8  
6.9  
5.5  
13.6  
10.6  
8.5  
26.3  
0.63  
0.8  
1
10  
23.8  
18.7  
14.9  
11.9  
9.3  
6.5  
8.2  
36.5  
29  
12.7  
15.9  
19.9  
25.5  
31.8  
39.8  
51  
10.3  
12.9  
16.5  
20.6  
25.7  
32.9  
41.2  
23  
1.25  
1.6  
2
18.4  
14.4  
11.5  
9.2  
7.5  
2.5  
3.2  
4
6
4.7  
7.2  
63.7  
3.7  
5.8  
0.63  
0.8  
1
16  
14.9  
11.8  
9.4  
7.5  
5.9  
4.7  
3.8  
2.9  
2.4  
10.4  
13.2  
16.5  
20.6  
26.3  
32.9  
41.2  
52.7  
65.8  
23  
20.4  
25.5  
31.8  
40.8  
51  
18.2  
14.5  
11.7  
9
1.25  
1.6  
2
40×/6B  
7.3  
5.8  
4.6  
3.6  
2.5  
3.2  
4
63.7  
81.5  
101.9  
Leica M Stereomicroscopes User Manual  
Download from Www.Somanuals.com. All Manuals Search And Download.  
63  
Optical data of Leica MZ75, MZ95  
1× Plan  
1.6× Planapo** 0.63×Planapo** 0.5× Plan**  
1× Planapo**  
0.32× Achromat 0.5× Achromat 1.5× Achromat  
1× Achromat  
0.8× Plan**  
2× Achromat  
0.8×Achromat 0.63× Achromat  
Objectives  
Working distance (mm)  
81 Plan  
55 Planapo  
19 Planapo  
97 Planapo  
135 Plan  
297 Achromat 187 Achromat 49 Achromat  
89 Achromat  
112 Plan  
27 Achromat  
112 Achromat 149 Achromat  
Eyepieces  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
6.3  
8
10  
33.3  
26.3  
21  
16.8  
13.1  
10.5  
8.4  
6.6  
5.3  
4.2  
3.5  
7.9  
10  
12.5  
15.6  
20  
25  
31.3  
40  
26.6  
21  
16.8  
13.5  
10.5  
8.4  
6.7  
5.3  
4.2  
3.4  
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
16.7  
5
6.4  
8
10  
12.8  
16  
20  
25.6  
32  
40  
48  
42  
3.9  
5
6.3  
7.8  
53.8  
42  
33.3  
26.9  
21  
16.8  
13.5  
10.5  
8.4  
2
105  
84  
67.7  
53.8  
42  
33.3  
26.9  
21  
3.2  
4
5
6.3  
8
10  
12.5  
16  
20  
25  
30  
65.6  
52.5  
42  
33.3  
26.3  
21  
16.8  
13.1  
10.5  
8.4  
9.4  
11.9  
14.9  
18.7  
23.9  
29.9  
37.3  
47.8  
59.7  
74.6  
89.6  
22.3  
17.6  
14.1  
11.2  
8.8  
7
5.6  
4.4  
3.5  
2.8  
2.3  
13.1  
10.5  
8.4  
6.6  
5.3  
4.2  
3.3  
2.6  
2.1  
1.8  
32.8  
26.3  
21  
16.4  
13.1  
10.5  
8.2  
6.6  
5.3  
4.4  
2.5  
3.1  
3.9  
5
6.3  
7.8  
12.5  
16  
20  
25  
32  
40  
50  
60  
10  
10×/21B  
16×/14B  
25×/9.5B  
40×/6B  
12.5  
15.6  
20  
25  
31.3  
37.5  
10  
50  
62.5  
75  
12.5  
15.6  
18.8  
16.8  
13.5  
11.2  
5
6*  
100  
120  
6.7  
5.6  
2.8  
7
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
10.1  
12.8  
16  
20  
25.6  
32  
40  
51.2  
64  
80  
96  
22.2  
17.5  
14  
11.2  
8.8  
7
5.6  
4.4  
3.5  
2.8  
2.3  
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
17.8  
14  
11.2  
9
20.2  
25.6  
32  
40  
51.2  
64  
11.1  
8.8  
7
8.1  
10.2  
12.8  
16  
20.5  
25.6  
32  
41  
51.2  
64  
27.7  
22  
17.5  
14  
10.9  
8.8  
7
5.5  
4.4  
3.5  
2.9  
6.3  
8
35.6  
28  
22.4  
17.9  
14  
11.2  
9
7
3.2  
4
5
6.3  
8
10  
12.5  
16  
20  
25  
30  
70  
56  
5
6.4  
8
10  
12.8  
16  
20  
25.6  
32  
40  
48  
44.8  
35  
28  
22.4  
17.5  
14  
11.2  
8.8  
7
15  
14.9  
11.7  
9.4  
7.5  
5.9  
4.7  
3.8  
2.9  
2.3  
1.9  
1.6  
19.1  
23.9  
29.9  
38.2  
47.8  
59.7  
76.4  
95.5  
119.4  
143.3  
10  
12.5  
16  
20  
25  
32  
40  
50  
60  
44.8  
35.6  
28  
22.4  
17.9  
14  
11.2  
9
7.5  
5.6  
4.4  
3.5  
2.8  
2.2  
1.8  
1.4  
1.2  
7
5.6  
4.5  
3.5  
2.8  
2.2  
1.9  
80  
102.4  
128  
160  
192  
5.6  
4.5  
3.7  
5
6*  
100  
120  
5.6  
4.7  
76.8  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
15.8  
20  
25  
31.3  
40  
50  
62.5  
80  
100  
125  
150  
15  
19.7  
25  
12.1  
9.5  
7.6  
6.1  
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.3  
31.5  
40  
50  
62.5  
80  
100  
125  
160  
200  
250  
300  
7.5  
5.9  
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.2  
1
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
18.8  
14.8  
11.9  
9.5  
7.4  
5.9  
4.8  
3.7  
3
9.8  
12.5  
15.6  
19.5  
25  
31.3  
39.1  
50  
24.2  
19  
4.9  
6.3  
7.8  
48.5  
37.7  
30.4  
24.2  
19  
15.2  
12.2  
9.5  
7.9  
10  
12.5  
15.6  
20  
25  
31.3  
40  
30.1  
23.8  
19  
15.2  
11.9  
9.5  
7.6  
5.9  
4.8  
3.8  
23.5  
29.9  
37.3  
46.6  
59.7  
74.6  
93.3  
119.4  
149.3  
186.6  
223.9  
10.1  
7.9  
6.4  
5.1  
4
3.2  
2.5  
2
11.9  
9.5  
7.6  
5.9  
4.8  
3.8  
3
2.4  
1.9  
1.6  
31.3  
39.1  
50  
62.5  
78.1  
100  
125  
156.3  
187.5  
15.2  
12.2  
9.5  
7.6  
6.1  
4.8  
3.8  
3
9.8  
12.5  
15.6  
19.5  
25  
31.3  
39.1  
46.9  
62.5  
78.1  
93.8  
7.6  
6.1  
5.1  
50  
62.5  
75  
1.6  
1.3  
1.1  
5
6*  
100  
120  
2.4  
2
0.8  
2.5  
3.2  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
25.2  
32  
40  
50  
64  
9.5  
7.5  
6
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.2  
1
31.5  
40  
50  
62.5  
80  
100  
125  
160  
200  
250  
300  
7.6  
6
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.2  
1
50.4  
64  
80  
4.8  
3.8  
3
20.2  
25.6  
32  
40  
51.2  
64  
11.9  
9.4  
7.5  
6
4.7  
3.8  
3
2.3  
1.9  
1.5  
1.3  
15.8  
20  
25  
31.3  
40  
50  
62.5  
80  
100  
125  
150  
15.2  
12  
9.6  
7.7  
6
4.8  
3.8  
3
2.4  
1.9  
1.6  
7.9  
10  
12.5  
15.6  
20  
25  
31.3  
40  
30.4  
24  
19.2  
15.4  
12  
9.6  
7.7  
6
4.8  
3.8  
3.2  
12.6  
16  
20  
25  
32  
40  
50  
64  
80  
19  
15  
12  
9.6  
7.5  
6
4.8  
3.8  
3
37.6  
47.8  
59.7  
74.6  
95.5  
119.4  
149.3  
191  
238.8  
298.5  
358.2  
6.4  
5
4
3.2  
2.5  
2
1.6  
1.3  
1
100  
128  
160  
200  
256  
320  
400  
480  
2.4  
1.9  
1.5  
1.2  
0.9  
0.8  
0.6  
0.5  
80  
100  
128  
160  
200  
240  
80  
102.4  
128  
160  
192  
50  
62.5  
75  
5
6*  
100  
120  
2.4  
2
0.8  
0.7  
0.8  
* Position 6 for MZ95 only  
** When using the planachromatic and planapochromatic objectives MZ125, the magnification is increased  
by the factor 1.25×.  
64  
Leica M Stereomicroscopes User Manual  
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Ergo objective  
0.4×–0.63×  
Objectives  
Working distance (mm)  
63.5 mm  
153.5mm  
Eyepieces  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
4
5
6.4  
8.0  
10.2  
12.7  
15.9  
20.4  
25.5  
31.8  
38.2  
52.5  
41.2  
32.8  
26.3  
20.6  
16.5  
13.2  
10.3  
8.2  
2.6  
3.3  
4
5
6.6  
80.8  
63.6  
51.2  
41.2  
31.8  
25.6  
20.4  
15.9  
12.7  
10.2  
8.5  
10×/21B  
16×/14B  
25×/9.5B  
40×/6B  
8.2  
10.3  
13.2  
16.5  
20.6  
24.7  
5
6*  
6.6  
5.5  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
6.4  
8.2  
35  
27.3  
22  
4
5.3  
6.6  
8.2  
10.5  
13.2  
16.5  
21  
26.3  
32.9  
39.5  
54.6  
42.3  
33.9  
27.3  
21.3  
17  
13.6  
10.6  
8.5  
10.2  
12.7  
16.3  
20.4  
25.5  
32.6  
40.8  
51  
17.6  
13.7  
11  
8.8  
6.9  
5.5  
4.4  
3.7  
5
6*  
6.8  
5.7  
61  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
10  
23.8  
18.7  
14.9  
11.9  
9.3  
7.5  
6
4.7  
3.7  
3
6.5  
8.2  
36.5  
29  
23  
18.4  
14.4  
11.5  
9.2  
7.2  
5.8  
4.6  
3.8  
12.7  
15.9  
19.9  
25.5  
31.8  
39.8  
51  
63.7  
79.6  
95.5  
10.3  
12.9  
16.5  
20.6  
25.7  
32.9  
41.2  
51.4  
61.7  
5
6*  
2.5  
0.63  
0.8  
1
1.25  
1.6  
2
2.5  
3.2  
4
16  
14.9  
11.8  
9.4  
7.5  
5.9  
4.7  
3.8  
2.9  
2.4  
1.9  
1.6  
10.4  
13.2  
16.5  
20.6  
26.3  
32.9  
41.2  
52.7  
65.8  
82.3  
98.8  
23  
20.4  
25.5  
31.8  
40.8  
51  
63.7  
81.5  
101.9  
127.4  
152.9  
18.2  
14.5  
11.7  
9
7.3  
5.8  
4.6  
3.6  
2.9  
2.4  
5
6*  
Leica M Stereomicroscopes User Manual  
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65  
Optical data of Leica MZ125, MZ16, MZ16 A  
1× Plan  
0.5× Plan  
0.63× Planapo  
0.8× Plan  
1.6× Planapo  
2× Planapo  
Objectives  
1× Planapo  
Working distance (mm)  
60 Plan  
135 Plan  
97 Planapo  
112 Plan  
19 Planapo  
15 Planapo  
55 Planapo  
Eyepieces  
0.71  
0.8  
1
7.1  
8
29.6  
26.3  
21  
3.5  
4
59.1  
52.5  
42  
4.5  
5.1  
47  
5.7  
6.4  
8
37  
11.4  
12.8  
16  
18.5  
16.4  
13.1  
10.5  
8.2  
14.2  
16  
14.8  
13.1  
10.5  
8.4  
6.6  
5.3  
4.2  
3.3  
2.6  
2.1  
1.7  
1.3  
1.1  
0.9  
41.2  
32.8  
26.3  
20.6  
16.4  
13.1  
10.2  
8.2  
32.8  
26.3  
21  
10  
5
6.4  
20  
1.25  
1.6  
2
12.5  
16  
16.8  
13.1  
10.5  
8.4  
6.3  
8
33.3  
26.3  
21  
8
10  
20  
25  
10.2  
12.8  
16  
12.8  
16  
16.4  
13.1  
10.5  
8.2  
25.6  
32  
32  
20  
10  
6.6  
40  
2.5  
3.2  
4
25  
12.5  
16  
16.8  
13.1  
10.5  
8.4  
20  
40  
5.3  
50  
10×/21B  
16×/14B  
25×/9.5B  
40×/6B  
32  
6.6  
20.5  
25.6  
32  
25.6  
32  
51.2  
64  
4.1  
64  
40  
5.3  
20  
25  
6.6  
3.3  
80  
5
50  
4.2  
6.6  
40  
5.3  
80  
2.6  
100  
126  
160  
200  
230  
6.3  
8
63  
3.3  
31.5  
40  
6.7  
40.3  
51.2  
64  
5.2  
50.4  
64  
4.2  
100.8  
128  
160  
184  
2.1  
80  
2.6  
5.3  
4.1  
3.3  
1.6  
10  
100  
115  
2.1  
50  
4.2  
3.3  
80  
92  
2.6  
1.3  
11.5  
1.8  
57.5  
3.7  
72.5  
2.9  
2.2  
1.1  
0.71  
11.4  
12.8  
16  
19.7  
17.5  
14  
5.7  
6.4  
8
39.4  
35  
7.2  
8.2  
31.3  
27.3  
22  
9.1  
10.2  
12.8  
16  
24.6  
22  
18.2  
20.5  
25.6  
32  
12.3  
10.9  
8.8  
7
5.5  
4.4  
3.5  
2.7  
2.2  
1.8  
1.4  
1.1  
0.9  
0.8  
22.8  
25.6  
32  
9.8  
8.8  
7
0.8  
1
28  
10.2  
12.8  
16.4  
20.5  
25.6  
32.8  
41  
17.5  
14  
1.25  
1.6  
2
20  
11.2  
8.8  
7
10  
22.4  
17.5  
14  
11.2  
8.8  
7
5.6  
4.4  
3.5  
2.8  
2.4  
17.5  
13.7  
10.9  
8.8  
40  
5.6  
4.4  
3.5  
2.8  
2.2  
1.8  
1.4  
1.1  
0.9  
0.7  
0.6  
25.6  
32  
12.8  
16  
20.5  
25.6  
32  
10.9  
8.8  
7
5.5  
4.4  
3.5  
2.8  
2.2  
1.8  
1.5  
41  
51.2  
64  
80  
102.4  
128  
160  
201.6  
256  
320  
368  
51.2  
64  
2.5  
3.2  
4
40  
5.6  
4.4  
3.5  
2.8  
2.2  
1.8  
1.4  
1.2  
20  
51.2  
64  
25.6  
32  
6.8  
41  
51.2  
64  
80.6  
102.4  
128  
147.2  
81.9  
102.4  
128  
5.5  
5
80  
40  
51.2  
64.5  
81.9  
102.4  
115.9  
4.4  
6.3  
8
100.8  
128  
160  
184  
50.4  
64  
3.5  
161.3  
204.8  
256  
2.7  
10  
80  
92  
2.2  
11.5  
1.9  
294.4  
0.71  
17.7  
20  
13.4  
11.9  
9.5  
7.6  
5.9  
4.8  
3.8  
3
8.9  
10  
26.8  
23.8  
19  
11.1  
12.8  
16  
21.2  
18.6  
14.8  
11.9  
9.3  
7.4  
5.9  
4.6  
3.7  
3
2.4  
1.9  
1.5  
1.3  
14.2  
16  
16.7  
14.8  
11.9  
9.5  
7.4  
5.9  
4.8  
3.7  
3
2.4  
1.9  
1.5  
1.2  
1
28.3  
32  
8.4  
7.4  
5.9  
4.8  
3.7  
3
2.4  
1.9  
1.5  
1.2  
0.9  
0.7  
0.6  
0.5  
35.4  
40  
6.7  
5.9  
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.2  
1
0.8  
0.6  
0.5  
0.4  
0.8  
1
25  
12.5  
15.6  
20  
20  
40  
50  
1.25  
1.6  
2
31.3  
40  
15.2  
11.9  
9.5  
7.6  
5.9  
4.8  
3.8  
3
20  
25  
50  
62.5  
80  
25.6  
32  
32  
64  
50  
25  
40  
80  
100  
125  
160  
200  
250  
315  
400  
500  
575  
2.5  
3.2  
4
62.5  
80  
31.3  
40  
40  
50  
100  
128  
160  
200  
252  
320  
400  
460  
51.2  
64  
64  
100  
125  
157.5  
200  
250  
287.5  
2.4  
1.9  
1.5  
1.2  
1
50  
80  
5
62.5  
78.8  
100  
125  
143.8  
80  
100  
126  
160  
200  
230  
6.3  
8
100.8  
128  
160  
181.1  
2.4  
1.9  
1.7  
10  
11.5  
0.8  
0.71  
28.4  
32  
8.4  
7.5  
6
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.2  
1
0.8  
0.6  
0.5  
14.2  
16  
16.9  
15  
12  
9.6  
7.5  
6
4.8  
3.8  
3
2.4  
1.9  
1.5  
1.2  
1
17.9  
20.5  
25.6  
32  
41  
51.2  
64  
13.4  
11.7  
9.4  
7.5  
5.9  
4.7  
3.8  
2.9  
2.3  
1.9  
1.5  
1.2  
0.9  
0.8  
22.7  
25.6  
32  
10.5  
9.4  
7.5  
6
4.7  
3.8  
3
2.3  
1.9  
1.5  
1.2  
0.9  
0.8  
0.6  
45.4  
51.2  
64  
5.3  
4.7  
3.8  
3
2.3  
1.9  
1.5  
1.2  
0.9  
0.8  
0.6  
0.5  
0.4  
0.3  
56.8  
64  
80  
4.2  
3.8  
3
0.8  
1
40  
20  
1.25  
1.6  
2
50  
25  
40  
80  
100  
128  
160  
200  
256  
320  
400  
504  
640  
800  
920  
2.4  
1.9  
1.5  
1.2  
0.9  
0.8  
0.6  
0.5  
0.4  
0.3  
0.3  
64  
32  
51.2  
64  
102.4  
128  
80  
40  
2.5  
3.2  
4
100  
128  
160  
200  
252  
320  
400  
460  
50  
80  
160  
64  
81.9  
102.4  
128  
102.4  
128  
160  
201.6  
256  
320  
368  
204.8  
256  
80  
5
100  
126  
160  
200  
230  
320  
6.3  
8
161.3  
204.8  
256  
403.2  
512  
10  
640  
736  
11.5  
289.8  
* Zoom positions 0.71 and 11.5 only for MZ16/MZ16 A  
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Dimensions of Leica MS5  
with incident-light stand  
with transmitted-light stand  
1
1× Achromat  
1× Achromat  
Measurements in mm  
MS5  
MS5  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Dimensions of Leica MZ6  
with incident-light stand  
with transmitted-light stand  
1
1× Achromat  
1× Achromat  
Measurements in mm  
MZ6  
MZ6  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Dimensions of Leica MZ75  
with incident-light stand  
with transmitted-light stand  
1
×
P
la
n
1
1× Plan  
Measurements in mm  
MZ75  
MZ75  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Dimensions of Leica MZ95  
with incident-light stand  
with transmitted-light stand  
1
×
P
la
n
1× Plan  
Measurements in mm  
MZ95  
MZ95  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Dimensions of Leica MZ125  
with incident-light stand  
with transmitted-light stand  
1× Plan  
1× Plan  
Measurements in mm  
MZ125  
MZ125  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Dimensions of Leica MZ16  
with incident-light stand  
with transmitted-light stand  
1x Planapo  
1x Planapo  
Measurements in mm  
MZ16  
MZ16  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Dimensions of Leica MZ16 A  
with incident-light stand  
with transmitted-light stand  
Measurements in mm  
Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.  
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Technical data electronic devices  
Transmitted-light base 20 W – Technical Data  
Type description  
Power supply  
Type of bulb  
Transmitted light base 20 W  
ES 635 474 incl. potentiometer  
Halogen bulb 6 V/20 W  
Connection for one external incident illumination with a maximum combined loading of 20 W  
Socket  
Operating voltage  
Primary voltage: 115 /-230 V, 50/60 Hz  
Secondary voltage: 7 V 28 VA  
Fuses, primary  
Switch positions  
For 115 V: 315 m AT / for 230 V: 160 m AT  
Position I: Transmitted light 6 V/20 W  
Position II: External incident lamp 20 W  
Operating environmental  
conditions:  
– Ambient temperature  
– Relative humidity  
– Atmospheric pressure  
+ 10° C ... + 35° C  
Ambient temperature up to 35° C: 75%  
700 ... 1060 hPa  
Transport and storage:  
– Temperature  
–20° C ... + 52° C  
– Relative humidity  
– Atmospheric pressure  
10 ... 95 % (non condensing)  
500 ... 1200 hPa  
Protection class  
Symbols  
Class II  
Insulation, Protection class II  
Safety transformer, protection class III  
SEV mark of conformity, reference number OG 2  
Regulating transformer – Technichal data  
Model code  
Outlet  
Operating voltage  
Transformer MTR38  
2× Connection for an external incident illuminator up to 20 W  
Primary: 100–240 VAC, 47–63Hz, 1.8 A  
Secondary: 5.3 V DC–7.5V/40 VA  
Primary fuse:  
2× T 1A L 250 V  
Ambient conditions  
during operation:  
– Ambient temperatures  
+ 10° C ... + 35° C  
– Rel. humidity  
– Atmospheric pressure  
Up to 35° C ambient temperature: 75%  
700 ... 1060 hPa  
Transport and storage:  
– Temperature  
–20° C ... + 52° C  
– Rel. humidity  
– Atmospheric pressure  
10 ... 95 % (non-condensing)  
500 ... 1200 hPa  
Protection class  
Symbols  
IP 30 according to EN 60529/IEC 529  
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Leica Microsystems – the brand  
for outstanding products  
The companies of the Leica Microsystems  
Group operate internationally in four business  
segments, where we rank with the market  
leaders.  
Leica Microsystems’ mission is to be the world’s first-choice provider of innovative  
solutions to our customers’ needs for vision, measurement, lithography and analysis  
of microstructures.  
Leica, the leading brand for microscopes and scientific instruments, developed from  
five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge  
Instruments. Yet Leica symbolizes innovation as well as tradition.  
Microscopy Systems  
Our expertise in microscopy is the basis for all  
our solutions for visualization, measurement  
and analysis of microstructures in life sciences  
and industry. With confocal laser technology  
and image analysis systems, we provide three-  
dimensional viewing facilities and offer new  
solutions for cytogenetics, pathology and mate-  
rials sciences.  
Leica Microsystems – an international company  
with a strong network of customer services  
Australia:  
Austria:  
Canada:  
China:  
Denmark:  
France:  
Gladesville, NSW  
Vienna  
Tel. +1 800 625 286  
Tel. +43 1 486 80 50 0 Fax +43 1 486 80 50 30  
Fax +61 2 9817 8358  
Richmond Hill/Ontario Tel. +1 905 762 20 00 Fax +1 905 762 89 37  
Hong Kong  
Herlev  
Rueil-Malmaison  
Cédex  
Bensheim  
Milan  
Tel. +8522 564 6699  
Tel. +45 44 5401 01  
Fax +8522 564 4163  
Fax +45 44 5401 11  
Specimen Preparation  
We provide comprehensive systems and ser-  
vices for clinical histo- and cytopathology  
applications, biomedical research and indus-  
trial quality assurance. Our product range  
includes instruments, systems and consum-  
ables for tissue infiltration and embedding,  
microtomes and cryostats as well as auto-  
mated stainers and coverslippers.  
Tel. +33 1 4732 8585  
Tel. +49 6251 1360  
Tel. +39 02 57 486 1  
Fax +33 1 4732 8586  
Fax +49 6251 136 155  
Fax +39 02 5740 3273  
Germany:  
Italy:  
Japan:  
Korea:  
Tokyo  
Seoul  
Tel. +81 3 543 596 09 Fax +81 3 543 596 15  
Tel. +82 2 514 6543 Fax +82 2 514 6548  
Netherlands:  
Portugal:  
Singapore:  
Spain:  
Sweden:  
Switzerland:  
Rijswijk  
Lisbon  
Tel. +31 70 41 32 130 Fax +31 70 41 32 109  
Tel. +35 1 213 814 766 Fax +35 1 213 854 668  
Medical Equipment  
Tel. +65 6 77 97 823  
Tel. +34 93 494 9530  
Tel. +46 8 625 45 45  
Fax +65 6 77 30 628  
Fax +34 93 494 9532  
Fax +46 8 625 45 10  
Innovative technologies in our surgical micro-  
scopes offer new therapeutic approaches in  
microsurgery.  
Barcelona  
Sollentuna  
Glattbrugg  
Tel. +41 44 809 34 34 Fax +41 44 809 34 44  
Tel. +44 1908 246 246 Fax +44 1908 609 992  
United Kingdom: Milton Keynes  
USA:  
Semiconductor Equipment  
Bannockburn/Illinois Tel. +1 800 248 0123  
Fax +1 847 405 0164  
Our automated, leading-edge measurement and  
inspection systems and our E-beam lithography  
systems make us the first choice supplier for  
semiconductor manufacturers all over the world.  
and representatives of Leica Microsystems  
in more than 100 countries.  
In accordance with the ISO 9001 certificate, Leica Microsystems (Switzerland) Ltd,  
Business Unit Stereo & Macroscope Systems has at its disposal a management system  
that meets the requirements of the international standard for quality management. In  
addition, production meets the requirements of the international standard ISO 14001 for  
environmental management.  
Leica Microsystems (Switzerland) Ltd  
Stereo & Macroscope Systems  
CH-9435 Heerbrugg  
Telephone +41 71 726 33 33  
Fax +41 71 726 33 99  
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