MICROSCOPE
COMPONENTS GUIDE
Choosing The Ideal UIS2 Optics Components For Your Equipment
2 0 0 8 - 1 0
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CONTENTS
WELCOME TO UIS2/UIS OPTICS ---------------------------------------------- 3 — 4
EYEPIECES/FILAR MICROMETER EYEPIECE --------------------------------- 33
Widefield eyepieces
WHN10x, WHN10x-H,
CROSSWHN10x, WH15x --------------------- 33
SWH10x-H, MICROSWH10x,
CROSSSWH10x ------------------------------ 33
U-OSM --------------------------------------- 33
SYSTEM DIAGRAM --------------------------------------------------------------- 5 — 6
Super widefield eyepieces
Filar micrometer eyepiece
UIS2/UIS OBJECTIVE LENSES ----------------------------------------------- 7 — 18
NEW
M Plan Apochromat
M Plan SemiApochromat
MPLAPON series -------------------------------8
MPLFLN series -------------------------------- 9
REVOLVING NOSEPIECES---------------------------------------------------- 34 — 35
Quintuple revolving nosepiece U-5RE-2-------------------------------------- 34
Sextuple revolving nosepiece with slider slot for DIC
U-D6RE--------------------------------------- 34
Long WD M Plan SemiApochromat LMPLFLN series ---------------------------- 10
M Plan Achromat MPLN series --------------------------------- 11
LCD Long WD M Plan SemiApochromat
LCPLFLN-LCD series -------------------------12
Super Long WD M Plan Achromat SLMPLN series -------------------------------13
Sextuple revolving nosepiece with slider slot for DIC with ESD treatment
U-D6RE-ESD --------------------------------- 34
Septuple revolving nosepiece with slider slot for DIC
U-D7RE -------------------------------------- 34
Centerable quadruple revolving nosepiece with slider slot for DIC
U-P4RE -------------------------------------- 34
Centerable sextuple revolving nosepiece with slider slot for DIC
U-P6RE -------------------------------------- 34
Quintuple revolving nosepiece for BF/DF
NEW
IR Long WD M Plan Achromat
IR M Plan Achromat
M Plan SemiApochromat BD
M Plan SemiApochromat BDP
LMPlan-IR series -----------------------------14
MPlan-IR -------------------------------------14
MPLFLN-BD series ---------------------------15
MPLFLN-BDP series --------------------------16
Long WD M Plan SemiApochromat BD
LMPLFLN-BD series --------------------------17
MPLN-BD series ------------------------------18
M Plan Achromat BD
U-5BDRE ------------------------------------ 35
Quintuple revolving nosepiece for BF/DF with slider slot for DIC
U-D5BDRE ----------------------------------- 35
Sextuple revolving nosepiece for BF/DF with slider slot for DIC/
U-D6BDRE ----------------------------------- 35
Centerable quintuple revolving nosepiece
MICROSCOPE SYSTEM BXFM ---------------------------------------------- 19 — 22
BXFM frame
BXFM
BXFM-F -------------------------------------- 19
BXFM-F+BXFM-ILH+BXFM-ILHSPU---------- 20
BXFM-F+BXFM-ILHS ------------------------ 21
SZ2-STU2 ----------------------------------- 22
U-ST ----------------------------------------- 22
SZ-STL -------------------------------------- 22
BXFM-S
U-P5BDRE ----------------------------------- 35
Adapter to mount BF objectives
Universal stand type 2
Compact stand
Large stand
BD-M-AD ------------------------------------ 35
VIDEO CAMERA ADAPTERS ------------------------------------------------- 36 — 37
ILLUMINATION UNITS --------------------------------------------------------- 23 — 25
C-mount video camera ports
Video camera mount adapters
Video camera port
U-TV0.25xC, U-TV0.35xC-2,
U-TV0.5xC-3, U-TV0.63xC ------------------- 36
U-CMAD3, U-BMAD, U-SMAD,
U-TMAD, U-FMT ----------------------------- 37
U-TV1x-2 ------------------------------------ 37
Reflected light illuminator for BF/DF
BX-RLA2 ------------------------------------- 23
Universal reflected light illuminatorBX-URA2 ------------------------------------ 23
Reflected light illuminators for BF BX-KMA/BX-KMA-ESD ----------------------- 24
Reflected light illuminator for BF U-KMAS ------------------------------------- 25
MOTORIZED UNITS------------------------------------------------------------- 38 — 41
LAMP HOUSING & ACCESSORIES ----------------------------------------- 26 — 28
Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple
revolving nosepiece+100W halogen lamp housing
BX-RLAA+U-D6REMC+U-LH100-3 ---------- 38
75W xenon apo lamp housing
100W mercury apo lamp housing U-LH100HGAPO ----------------------------- 26
100W mercury lamp housing
100W halogen lamp housings
External power supply
Hand switch
Extension cord
DF converter for BX-URA2
Fiber adapter for reflected light observation
U-LGAD -------------------------------------- 27
Transmitted light guide adapter SZX-TLGAD ---------------------------------- 27
U-LH75XEAPO ------------------------------- 26
U-LH100HG ---------------------------------- 26
U-LH100-3/U-LH100IR/U-LH100L-3 -------- 26
TH4-100/200 -------------------------------- 27
TH4-HS -------------------------------------- 27
U-RMT --------------------------------------- 27
U-RCV --------------------------------------- 27
Motorized universal reflected light illuminator
BX-RFAA ------------------------------------- 38
Motorized sextuple BD revolving nosepiece with slider slot for DIC
U-D6BDREMC ------------------------------- 39
Motorized centerable quintuple revolving nosepiece with slider slot for DIC
U-P5BDREMC -------------------------------- 39
Motorized quintuple BD revolving nosepiece with slider slot for DIC
U-D5BDREMC ------------------------------- 39
NEW
NEW
Motorized sextuple revolving nosepiece with slider slot for DIC
U-D6REMC ---------------------------------- 39
Motorized centerable quintuple revolving nosepiece with slider slot for DIC
U-P5REMC ----------------------------------- 39
Light source
Light guide
Double lamp house adapter
LG-PS2 -------------------------------------- 28
LG-SF ---------------------------------------- 28
U-DULHA ------------------------------------ 28
Control unit
Hand switch
BX-UCB -------------------------------------- 39
U-HSTR2 ------------------------------------ 39
OBSERVATION TUBES -------------------------------------------------------- 29 — 30
Control box for motorized nosepiece and BF/DF illuminator
BX-REMCB ---------------------------------- 39
AC adapter for BX-REMCB
Active auto focus unit
Motorized reflected filter wheel U-FWR --------------------------------------- 40
Motorized illumination with power focus
Widefield trinocular tube
Widefield trinocular tube for IR
Widefield erect image trinocular tube
U-TR30-2 ------------------------------------ 29
U-TR30IR ------------------------------------ 29
U-ACAD4515 -------------------------------- 39
U-AFA2M-VIS -------------------------------- 40
NEW
U-ETR-4 ------------------------------------- 29
U-TLU ---------------------------------------- 29
Single port tube with lens
Single port tube with lens for IR U-TLUIR ------------------------------------- 29
Super widefield trinocular tube U-SWTR-3 ----------------------------------- 30
Super widefield erect image trinocular tube
U-SWETR ------------------------------------ 30
Super widefield erect image tilting trinocular tube
MX-SWETTR --------------------------------- 30
BXFMA-F ------------------------------------ 41
DEEP ULTRAVIOLET OBSERVATION SYSTEM -------------------------------- 42
UV248 compatible intermediate tube
U-UVF248IM --------------------------------- 42
UV quartz light guide
UV248 compatible light source box + Mercury Xenon lamp housing
U-UVF248LB+U-LH80HBXE ----------------- 42
U-UVF2FV/5FB ------------------------------- 42
INTERMEDIATE TUBES & ACCESSORIES ------------------------------- 31 — 32
Magnification changer
Magnification changer 2x
Trinocular intermediate attachment
U-CA ----------------------------------------- 31
U-ECA --------------------------------------- 31
OPTICAL TERMINOLOGY ---------------------------------------------------- 43 — 47
U-TRU --------------------------------------- 31
U-DP ----------------------------------------- 32
U-DP1xC ------------------------------------- 32
U-EPA2 -------------------------------------- 32
U-APT --------------------------------------- 32
Dual port
Dual port 1x
Eyepoint adjuster
Arrow pointer
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2
WELCOME TO UIS2/UIS OPTICS
UIS2/UIS:
The System That Maximizes The Advantage Of Infinity-Corrected Optics
What's infinity-corrected optics?
light between the objective lens and tube lens,
allowing the creation of user-specific or task-
specific optical systems. To establish real flexibility
with such a system, it is necessary to eliminate the
occurrence of coma aberration.
UIS2/UIS optics is an infinity-corrected optical
system — in other words, a system in which light
passes from the specimen through the objective
lens without forming an image along the way.
Instead, it travels in the form of infinity parallel rays
to the tube lens. The tube lens is where the
intermediate image is formed, whereas in finite-
corrected optics, this is done by the objective lens.
*In UIS2/UIS objective lenses, the parfocal distance is designed
at 45mm and the focal length of the tube lens is 180mm.
Figure 2 Advantages of Infinity-corrected
optical system
Infinity-corrected optical system
Finite-corrected optical system
Figure 1 Infinity-corrected and
finite-corrected optical system principles
Infinity-corrected optical system
Objective
lens
Tube lens
Objective
lens
Eyepiece
Parallel light beam
UIS/UIS2
objective lens
Tube lens
Intermediate
image
Basic dimensions of UIS2/UIS optical system
Finite-corrected optical system
Eyepiece
The UIS2/UIS optical system optimally corrects
aberration with a dedicated telan lens and an
eyepiece so that the coma aberration and flatness
are not degraded even when the telan lens exit
pupil position is changed by changing the
objective lens and telan distance. This makes it
possible to use a distance of 50mm to 170mm
from objective lens mounting position to the single
port tube with lens.
Intermediate
image
Objective
lens
Advantages of infinity-corrected optics
This system, known as "infinity-corrected optics",
offers a number of advantages:
*Coma aberration: refer to the optical terminology at the end of
• There is no change in magnification even when
the distance between the objective lens and tube
lens is altered.
• With the total magnification remaining constant,
there is no image aberration — even when
prisms or sliders are interposed between the
objective lens and the tube lens.
this document.
Figure 3 Basic dimensions of UIS2/UIS2
optical system
U-TLU
(Single port tube with lens)
Objective lens
Image
* 40mm * 84mm
45mm Recommended distance
50-170mm
57.6mm
102mm
As thousands of users have found by experience,
these advantages are crucial to composing the
ideal microscope optical system. What's more, it
is even possible to freely insert or remove
*Basic dimensions when our revolving nosepiece and illuminator are
combined. When the position of the illuminator above is changed,
illumination performance cannot be maintained.
intermediate attachments in the parallel rays of
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3
WELCOME TO UIS2/UIS OPTICS
Features of UIS2 objective lenses
4. Lightening
Weight has been reduced to approximately 2/3
that of conventional products by using an
aluminum objective lens barrel cover. This has
the effect of lightening the load on the devices at
objective lens up/down, suppressing vibrations
by lowering the inertial force at objective lens
switching, etc. (MPLFLN series, LMPLFLN
series)
UIS2 objective lenses ensure compatibility (screw
diameter, optical performance) with the UIS optical
system and have the following features compared
to conventional objective lenses.
1. Wavefront aberration control
The Olympus UIS2 objective lenses set a new
standard, with wavefront aberration control in
addition to common performance standards of
N.A. and W.D. Olympus challenges farther
highest order optics which has not been fulfilled
by the conventional standards. We offer
excellent performance objective lenses by
minimizing the aberrations that lower resolution.
*Wave front aberration: refer to the optical terminology at the
end of this document.
5. Adoption of eco-lens
The glass materials of UIS2 objective lenses are
all lead- and cadmium-free eco-glass.
Based on our conviction that the UIS2/UIS
system is the best way to maximize the
advantages of infinity-corrected optical
systems, we confidently recommend the
UIS2/UIS-featured Olympus microscope units
for all your high-precision needs in research,
inspection and production equipment.
2. Objective lenses with excellent image
parcentricity
High power SemiApochromatic UIS2 objective
lenses make the centration tolerance between
objective lenses on the microscope nosepiece
keep the image within the enter of the field of
view even with digital cameras. (50x or higher
power in both MPLFLN and LMPLFLN series)
* Refer to the Olympus home page for detailed
objective lenses specifications.
3. Improvement of color reproducibility
UIS2 objective lenses realize natural color
reproduction without any chromatic shifts using
stringently selected high transmittance glass
and advanced coating technology that provides
high transmittance which is flat over an ultra-
wide band wavelength. In addition, since the
total optical system, including the tube lens is
designed to reproduce a natural color, clear
images faithful to the specimen are obtained
even with digital imaging.
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4
SYSTEM DIAGRAM
BXFM SYSTEM DIAGRAM
Video system
Refer to pages 36-37
Video camera
C-mount
Video camera
B mount 2/3"
Video camera
S mount 2/3"
Video camera
F mount
U-FMT
U-CMAD3
U-BMAD
U-SMAD
U-TMAD
U-TV0.25xC
U-TV0.35xC-2
U-TV0.5xC-3
U-TV0.63xC
U-TV1x-2
Observation/single tubes
and eyepieces
Refer to pages 29-30
WHN
Eyepieces
SWH
Eyepieces
Widefield trinocular
observation tubes
Super widefield trinocular
observation tubes
U-TLU
U-TLUIR
Illumination systems and power supply
U-25ND6, U-25ND25,
U-25LBD, U-25IF550,
U-25L42, U-25FR,
Revolving nosepiece
(Refer to pages 34-35)
U-AN360-3
U-AN360IR
U-AN
U-PO3
U-POIR
U-POTP3
U-BP1100IR, U-BP1200IR
Refer to pages 23-28
FS
FS
AS
AS
5
6
SHUTTER
ND
SZX-TLGAD
BX-KMA/
BX-KMA-ESD
BX-RLA2
BX-URA2
U-KMAS
U-RCV
LG-SF
LG-PS2
TH4-HS
U-LGAD
Refer to pages 19-21
BXFM-ILHS
Focusing units
U-DULHA
U-LH100-3
U-LH100L-3
U-LH100IR
TH4-100
BXFM-ILH
U-LH100HGAPO
U-LH100HG
BXFM-ILHSPU
U-LH75XEAPO
BXFM-F
Refer to page 22
Stands
SZ-STL
U-ST
SZ2-STU2
*Different types may be offered in each area.
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5
SYSTEM DIAGRAM
MOTORIZED UNIT SYSTEM DIAGRAM
Refer to pages 38-40
Refer to page 26
Observation tubes
(Refer to pages 29-30)
U-AN
MX-AFDIC
U-AN
U-AN360-3
U-FWO
Intermediate tubes
(Refer to pages 31-32)
U-PO3
U-AFA2M-VIS
U-LH75XEAPO
U-LH100HGAPO
U-LH100HG
U-POTP3
U-25ND6,
U-25ND25
U-25LBD
U-25IF550
U-25L42
U-25FR
Mirror units
U-LH100-3
U-LH100L-3
U-FWR
BX-RLAA
BX-RFAA
U-ZPCB
Z board
BX-UCB*
U-DICR
Refer to
page 35
Refer to pages 7-18
U-D6REMC
See manual
U-HSTR2
BF objective lenses
U-DICRH
BD-M-AD
U-D5BDREMC
U-D6BDREMC
PC
U-DICRHC
BF/DF objective lenses
analySIS FIVE
Control software
* BX-REMCB is also available for BX-RLAA + motorized revolving nosepiece control (refer to page 39)
BXFM-A SYSTEM DIAGRAM
Refer to page 41
Refer to page 26
ø32filter
Video system
(Refer to pages 37-38)
Auxiliary lens
(provided with the BXFMA-F)
Power source
U-LH100HG
U-LH75XEAPO
Observation tubes
(Refer to pages 29-30)
MX-AFDIC
U-AN
Refer to pages 27-28
U-AFA2M-VIS
LG-SF
LG-PS2
U-LGAD
Refer to pages 26-27
U-RMT
BXFMA-F
U-FWR
BX-UCB
U-LH100-3
U-LH100L-3
U-ZPCB
Z board
See manual
U-D6REMC
U-HSTR2
U-P5REMC
U-D5BDREMC
U-D6BDREMC
U-P6BDREMC
U-IFFH
PC
analySIS FIVE
Control software
Objective lenses
U-FH
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6
UIS2/UIS OBJECTIVE LENSES
■ Meaning of abbreviations shown on objective lens
M P L (Plan) F L N - 1 0 0
B D
M:
Metallurgical (no cover)
PL: Plan/
None: Achromat/
None: UIS
N: UIS2
Number:
Objective lens
magnification
None: Brightfield
BD: Brightfield/darkfield
Corrects field
Corrects aberration at 2 wavelengths of
LM: Long working distance
curvature of
the periphery of
the image plane
blue and red
metallurgical use
BDP: Brightfield/darkfield/
FL:
SemiApochromat/
Corrects chromatic aberration in the
visible range (violet~red)
SLM: Super long working
polarizing
distance metallurgical use
IR:
IR
LC: Observation through
LCD: LCD
APO: Apochromat/
substrate
Optimally corrects chromatic aberration
in the entire visible band (violet~red)
Objective lenses series abbreviation (PL: Plan)
N.A. (Numerical Aperture)
■ Objective lens notation
Magnification
Infinity-corrected optical system
Cover glass thickness (no cover)
For brightfield observation
Field Number
■ Objective lens series list
*1
Series
MPLAPON
MPLFLN
Magnification
50/100
BF
\
DF DIC
POL
\
FL
F.N. (Field Number)
26.5
Remarks
UIS2
\U
Use together with polarizer and
analyzer recommended
1.25/2.5
\
1.25x: 22 /2.5x: 26.5
*2
5/10/20/50/100
5/10/20/50/100
5/10/20/50/100
20/50/100
20/50/100
5/10/20/50/100 *
5/10/20/50/100/150
5/10/20/50/100
5/10/20/50/100
5/10/20/50/100
\
\
\
\
\
\
\
\
\
\
\U
\L
\
\
\
26.5
26.5
22
LMPLFLN
MPLN
LCPLFLN-LCD
SLMPLN
LMPlanIR/MPlanIR
MPLFLN-BD
MPLFLN-BDP
LMPLFLN-BD
MPLN-BD
\
\L
26.5
26.5
22
26.5
26.5
26.5
22
For LCD
4
UIS
UIS2
For near-IR observation
*2
\
\
\
\
\U
aU
\L
\
a
\
\
*2
\
\
1
2
* DIC prism U-DICR: UM/LM position, U-DICRHC: LM position fixed, U-DICRH: UM position fixed. * 5~20x: U excitation also possible
3
4
* 50x: DIC observation not applicable * MPlanIR: available 100x only \: Responds a: Optimally responds BF: Brightfield DF: Darkfield
DIC: Differential Interference Contrast POL: Polarized light FL: Fluorescence
■ Features of objective lens series
a MPLAPON: Plan Apochromat — P 8
a SLMPLN series: Super Long WD M Plan Achromat — P 13
Plan Achromat objective lenses with high magnification and super long working distance.
Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses,
select from the LMPLFLN Series.
Plan Apochromat objective lenses that correct chromatic aberrations at the highest level.
Olympus guarantees*1 optical performance (wavefront aberration) with a Strehl ratio*2
of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit.
*1 Definition of guaranteed values: Measurements assessed with Olympus’ Interferometer for
Transmitted Wavefront Measurement under specified conditions (measurement: temp.
23°C 1°C; assessment: measurement within the 97% range of the pupil dia.).
a LMPlan-IR series: IR Long WD M Plan Achromat — P 14
MPlan-IR: IR M Plan Achromat — P 14
IR objective lenses which compensate for aberrations from visible to near infrared light.
Ideal for the observations of semiconductor interiors and the back surface of a chip package
as well as CSP bump inspection.
*2 Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual
optical system can concentrate with respect to the proportion of light concentrated in the
image plane (central intensity) by an ideal, aberration-free optical system, with the latter
serving as 100%. A higher percentage indicates a higher quality optical system.
a MPLFLN-BD series: M Plan SemiApochromat BD — P 15
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification.
a MPLFLN series: M Plan SemiApochromat — P 9
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The lineup consists of 7 objective lenses ranging from 1.25x to 100x, and secures a W.D. of
1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized,
the position of the DIC prism does not have to be switched when changing the magnification.
For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the
reflected light illuminator.
a MPLFLN-BDP series: M Plan SemiApochromat BDP — P 16
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification. The BDP series optimizing brightfield/darkfield and
polarized light characteristics is perfect for Nomarski DIC and polarized light observations.
a LMPLFLN series: Long WD M Plan SemiApochromat — P 10
Long working distance Plan SemiApochromat objective lenses, giving high-level correction
for chromatic aberration. Suitable with samples having a height difference and in preventing
collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification.
a LMPLFLN-BD series: Long WD M Plan SemiApochromat BD — P 17
Long working distance Plan SemiApochromat objective lenses, giving high-level correction
for chromatic aberration. Suitable with samples having a height difference and in preventing
collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification.
a MPLN series: M Plan Achromat — P 11
Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.
a LCPLFLN-LCD series: LCD Long WD M Plan SemiApochromat — P 12
Perfect objective lens series for observation of LCD panels and other samples through a
glass substrate. Aberration correction matched to the glass thickness is accomplished using
a correction ring.
a MPLN-BD series: M Plan Achromat BD — P 18
Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.
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7
UIS2 OBJECTIVE LENSES
Plan Apochromat
MPLAPON series
Plan Apochromat objective lenses that correct chromatic aberrations at the highest level.
Olympus guarantees*1 optical performance (wavefront aberration) with a Strehl ratio*2 of 95% or better.
They are also designed for use with Olympus' U-AFA2M active AF unit.
MPLAPON50x
MPLAPON100x
ø20.32
ø20.32
*1 Definition of guaranteed values: Measurements
assessed with Olympus’ Interferometer for
Transmitted Wavefront Measurement under specified
conditions (measurement: temp. 23°C 1°C;
assessment: measurement within the 97% range of
the pupil dia.).
*2 Strehl ratio: Indicates in percent (%) the ratio of the
proportion of light that an actual optical system can
concentrate with respect to the proportion of light
concentrated in the image plane (central intensity) by
an ideal, aberration-free optical system, with the latter
serving as 100%. A higher percentage indicates a
higher quality optical system.
ø8.8
ø18.9
ø20.3
ø28.5
ø5.5
ø16
ø20.2
ø28
Unit: mm
Widefield eyepiece WHN10x
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Field Number 22
Objective lens
(magnification)
Numerical
Aperture
Working distance Focal distance
Weight
(g)
Total
Practical field Depth of
Total
Practical field Depth of
(mm)
0.35
0.35
f (mm)
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
MPLAPON 50x
MPLAPON 100x
0.95
0.95
3.6
139
125
500
0.44
0.22
1.0
500
0.53
0.27
1.0
0.7
1.8
1000
0.67
1000
Screw: W20.32x0.706 (0.8"x1/36")
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8
UIS2 OBJECTIVE LENSES
M Plan SemiApochromat
MPLFLN series
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objective
lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective
lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification.
For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator.
MPLFLN1.25x*
MPLFLN2.5x*
MPLFLN5x
MPLFLN10x
ø28
ø28
ø20.32
ø20.32
ø20.32
ø20.32
ø28.6
ø29
ø24.5
ø29
ø20.9
ø26
ø14.5
ø26
ø30
ø30
MPLFLN20x
MPLFLN50x
MPLFLN100x
ø20.32
ø20.32
ø20.32
ø12.1
ø26
ø10.7
ø7.8
ø15.2
ø17.8
ø26
ø15.2
ø17.8
ø26
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Numerical
Working distance Focal distance
Objective lens
Weight
(g)
Total Practical field Depth of
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Total
Practical field Depth of
(magnification)
MPLFLN 1.25x*
MPLFLN 2.5x*
MPLFLN 5x
Aperture
(mm)
f (mm)
145
72
0.04
3.5
122
12.5
25
17.6
8.8
4.4
2.2
1.1
870
220
59
—
25
—
—
220
59
0.08
10.7
20.0
11.0
3.1
106
10.6
5.3
2.7
1.3
0.15
36
51.5
68.1
70.4
89.9
90.9
50
50
MPLFLN 10x
MPLFLN 20x
MPLFLN 50x
MPLFLN 100x
0.30
18
100
15
100
200
500
1000
15
0.45
9
200
500
5.2
5.1
0.80
1.0
3.6
1.8
0.44
0.22
1.3
0.53
0.27
1.3
0.90
1.0
1000
0.73
0.73
Screw: W20.32x0.706 (0.8"x1/36")
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9
UIS2 OBJECTIVE LENSES
Long WD M Plan SemiApochromat
(WD: Working Distance)
LMPLFLN series
Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
Suitable with samples having a height difference and in preventing collision, as the working distance is long.
Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have
to be switched when changing the magnification.
LMPLFLN5x
LMPLFLN10x
LMPLFLN20x
ø20.32
ø20.32
ø20.32
ø25.4
ø26
ø17
ø26
ø15.2
ø22.4
ø26
LMPLFLN50x
LMPLFLN100x
ø20.32
ø20.32
ø12.5
ø18.2
ø26
ø15
ø18.1
ø26
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance Focal distance
Weight
Total
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Practical field Depth of
Total
Practical field Depth of
(mm)
22.5
21.0
12.0
10.6
3.4
f (mm)
(g)
50
54
73
77
94
LMPLFLN 5x
LMPLFLN 10x
LMPLFLN 20x
LMPLFLN 50x
LMPLFLN 100x
0.13
36
50
100
4.4
70
18
50
100
5.3
70
18
0.25
18
2.2
2.7
0.40
9
200
1.1
6.1
200
1.3
6.1
0.50
3.6
1.8
500
0.44
0.22
2.5
500
0.53
0.27
2.5
0.80
1000
0.87
1000
0.87
Screw: W20.32x0.706 (0.8"x1/36")
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10
UIS2 OBJECTIVE LENSES
M Plan Achromat
MPLN series
Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.
MPLN5x
MPLN10x
MPLN20x
ø20.32
ø20.32
ø20.32
ø10.5
ø6
ø21
ø24
ø11.9
ø12
ø16
ø24
ø15.8
ø24
MPLN50x
MPLN100x
ø20.32
ø20.32
ø4.4
ø6
ø11.9
ø11.6
ø15.8
ø24
ø15.6
ø24
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance
(mm)
Focal distance
Weight
(g)
Total
magnifications
Practical field of Depth of focus
f (mm)
view (mm)
(µm)
MPLN
5x
0.10
20.0
10.6
1.3
36
64
80
50
100
4.4
98
MPLN
MPLN
MPLN
MPLN
10x
20x
50x
100x
0.25
18
2.2
18
0.40
9
111
113
116
200
1.1
6.1
1.4
0.73
0.75
0.38
0.21
3.6
1.8
500
0.44
0.22
0.90
1000
Screw: W20.32x0.706 (0.8"x1/36")
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11
UIS2 OBJECTIVE LENSES
LCD Long WD M Plan SemiApochromat
LCPLFLN-LCD series
Perfect objective lens series for observation of LCD panels and other samples through a glass substrate.
Aberration correction matched to the glass thickness is accomplished using a correction ring.
LCPLFLN20xLCD***
LCPLFLN50xLCD***
LCPLFLN100xLCD***
ø20.32
ø20.32
ø20.32
ø12.77
ø15
ø25
ø15.2
ø25
ø17.84
ø25
ø29.5
ø31
ø29.5
ø31
ø29.5
ø31
Unit: mm
* Value at glass thickness 0.7mm observation
Objective lens
LCPLFLN20xLCD
LCPLFLN50xLCD
LCPLFLN100xLCD
Corresponding glass thickness (mm)
0-1.2
0.7
0-1.2
0.7
0-0.7
0.5
Correction ring indication
Working distance (mm)
Correction system
0
1.2
7.4
0
1.2
2.2
0
0.7
0.9
8.3
7.8
3.0
2.5
1.2
0.98
Correction ring
Correction ring
Correction ring
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Objective lens
(magnification)
Numerical Working distance** Focal distance
Total
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Practical field Depth of
Total
Practical field Depth of
Weight
(g)
Aperture
(mm)
7.8
2.5
0.9
f (mm)
LCPLFLN 20xLCD***
LCPLFLN 50xLCD***
LCPLFLN 100xLCD***
0.45
9
146
170
185
200
500
1.1
5.2
200
500
1.3
5.2
0.70
3.6
0.44
0.22
1.6
0.53
0.27
1.6
0.85
1.8
1000
0.79
1000
0.79
Screw: W20.32x0.706 (0.8"x1/36")
**The figure shown here is the value when the correction ring indication is 0.7. *** To be available in the beginning of 2007
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12
UIS2 OBJECTIVE LENSES
Super Long WD M Plan Achromat
SLMPLN series
Plan Achromat objective lenses with high magnification and super long working distance.
Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN series.
SLMPLN20x
SLMPLN50x
SLMPLN100x
ø20.32
ø20.32
ø20.32
ø16.1
ø25
ø16.9
ø17.3
ø21.4
ø26
ø14.7
ø23.8
ø26
ø26
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance Focal distance
Weight
(g)
Total
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Practical field Depth of
Total
Practical field Depth of
(mm)
f (mm)
SLMPLN 20x
SLMPLN 50x
SLMPLN 100x
0.25
0.35
0.6
25
18
7.6
9
56
74
200
500
1.1
11.4
4.2
200
500
1.3
11.4
4.2
3.6
1.8
0.44
0.22
0.53
0.27
100
1000
1.3
1000
1.3
Screw: W20.32x0.706 (0.8"x1/36")
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13
UIS OBJECTIVE LENSES
IR Long WD M Plan Achromat/IR M Plan Achromat
LMPlan-IR series/MPlan-IR
UNIVERSAL
INFINITY SYSTEM
IR objective lenses which compensate for aberrations from visible to near infrared light.
Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection.
LMPlan5xIR
LMPlan10xIR
LMPlan20xIR
ø20.32
ø20.32
ø20.32
ø
21
26
ø12.4
ø10.4
ø
ø
15
22
ø
18
26
ø
ø
ø26
LMPlan50xIR
LMPlan100xIR
MPlan100xIR
ø20.32
ø20.32
ø20.32
ø12.5
ø15.6
ø12.5
ø4.95
ø12.2
ø
15
18.12
26
ø14.8
ø18
ø
ø26
ø26
ø
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
UIS objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance
(mm)
Focal distance
f (mm)
Weight
(g)
Total
magnifications
Practical field of Depth of focus
view (mm)
(µm)
98
18
6.1
LMPlan
5xIR
0.10
0.25
0.40
0.55
0.80
0.95
20.0
18.5
8.1
36
18
9
73
73
50
100
4.4
LMPlan
LMPlan
LMPlan
LMPlan
MPlan
10xIR
20xIR
50xIR
100xIR
100xIR
2.2
110
115
122
130
200
1.1
6.0
3.6
1.8
1.8
500
0.44
0.22
0.22
2.2
3.4
1000
1000
0.87
0.67
0.3
Screw: W20.32x0.706 (0.8"x1/36")
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14
UIS2 OBJECTIVE LENSES
M Plan SemiApochromat BD
(BD:Brightfield/Darkfield)
MPLFLN-BD series
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the
position of the DIC prism does not have to be switched when changing the magnification.
MPLFLN5xBD
MPLFLN10xBD
MPLFLN20xBD
ø26
ø26
ø26
ø16
ø22.4
ø29.5
ø32
ø16.8
ø22
ø17
ø22
ø26.2
ø27
ø27.5
ø28.5
ø32
ø32
MPLFLN50xBD
MPLFLN100xBD
MPLFLN150xBD
ø26
ø26
ø26
ø20
ø27.2
ø32
ø20
ø27.2
ø32
ø20
ø27.2
ø32
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance Focal distance
Weight
(g)
Total
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Practical field Depth of
Total
Practical field Depth of
(mm)
12.0
6.5
f (mm)
MPLFLN 5xBD
MPLFLN 10xBD
MPLFLN 20xBD
MPLFLN 50xBD
MPLFLN 100xBD
MPLFLN 150xBD
0.15
36
95.5
82.8
87.7
99.8
98.9
104.8
50
100
4.4
59
15
50
100
5.3
59
15
0.30
18
2.2
2.7
0.45
3.0
9
200
1.1
5.2
200
1.3
5.2
0.80
1.0
3.6
1.8
1.2
500
0.44
0.22
0.15
1.3
500
0.53
0.27
0.18
1.3
0.90
1.0
1000
1500
0.73
0.6
1000
1500
0.73
0.6
0.90
1.0
Screw: W26x0.706
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15
UIS2 OBJECTIVE LENSES
M Plan SemiApochromat BDP
(BDP:Brightfield/Darkfield/Polarizing)
MPLFLN-BDP series
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of
1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism
does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized
light characteristics is perfect for Nomarski DIC and polarized light observations
MPLFLN5xBDP
MPLFLN10xBDP
MPLFLN20xBDP
ø26
ø26
ø26
ø16.8
ø22
ø26
ø27
ø32
ø17
ø22
ø16
ø22.4
ø29.3
ø32
ø27.5
ø28.5
ø32
MPLFLN50xBDP
MPLFLN100xBDP
ø26
ø26
ø20
ø27.5
ø32
ø20
ø27.5
ø32
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance Focal distance
Weight
(g)
Total
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Practical field Depth of
Total
Practical field Depth of
(mm)
12.0
6.5
f (mm)
MPLFLN 5xBDP
MPLFLN 10xBDP
MPLFLN 20xBDP
MPLFLN 50xBDP
MPLFLN 100xBDP
0.15
36
95.5
83.3
50
100
4.4
59
18
50
100
5.3
59
18
0.25
18
2.2
2.7
0.40
3.0
9
88.5
200
1.1
6.1
200
1.3
6.1
0.75
1.0
3.6
1.8
100.5
101.5
500
0.44
0.22
1.4
500
0.53
0.27
1.4
0.90
1.0
1000
0.73
1000
0.73
Screw: W26x0.706
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16
UIS2 OBJECTIVE LENSES
Long WD M Plan SemiApochromat BD
LMPLFLN-BD series
Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
Suitable with samples having a height difference and in preventing collision, as the working distance is long.
Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have
to be switched when changing the magnification.
LMPLFLN5xBD
LMPLFLN10xBD
LMPLFLN20xBD
ø26
ø26
ø26
ø22
ø15.5
ø21.9
ø28.2
ø30
ø16.2
ø22.4
ø28
ø23
ø28
ø30
ø32
ø29.5
ø32
ø32
LMPLFLN50xBD
LMPLFLN100xBD
ø26
ø26
ø20.7
ø21
ø29
ø23
ø28
ø32
ø30.3
ø32
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
Super widefield eyepiece SWH10x
Field Number 26.5
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance Focal distance
Weight
(g)
Total
magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm)
Practical field Depth of
Total
Practical field Depth of
(mm)
15.0
10.0
12.0
10.6
3.3
f (mm)
LMPLFLN 5xBD
LMPLFLN 10xBD
LMPLFLN 20xBD
LMPLFLN 50xBD
LMPLFLN 100xBD
0.13
36
81
84
50
100
4.4
70
18
50
100
5.3
70
18
0.25
18
2.2
2.7
0.40
9
86
200
1.1
6.1
200
1.3
6.1
0.50
3.6
1.8
85
500
0.44
0.22
2.5
500
0.53
0.27
2.5
0.80
102
1000
0.87
1000
0.87
Screw: W26x0.706
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17
UIS2 OBJECTIVE LENSES
M Plan Achromat BD
MPLN-BD series
Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.
MPLN5xBD
MPLN10xBD
MPLN20xBD
ø26
ø26
ø26
ø16
ø29.3
ø30.5
ø32
ø16.8
ø22
ø26
ø27
ø32
ø17
ø23.6
ø29
ø32
MPLN50xBD
MPLN100xBD
ø26
ø26
ø10
ø10
ø20.7
ø23.1
ø29
ø23
ø27
ø29
ø32
ø32
Unit: mm
Widefield eyepiece WHN10x
Field Number 22
UIS2 objective lenses
Objective lens
(magnification)
Numerical
Aperture
Working distance
(mm)
Focal distance
f (mm)
Weight
(g)
Total
magnifications
Practical field of Depth of focus
view (mm)
(µm)
MPLN 5xBD
MPLN 10xBD
MPLN 20xBD
MPLN 50xBD
MPLN 100xBD
0.10
0.25
0.40
0.75
0.90
12.0
6.5
36
18
9
137
155
162
157
160
50
100
4.4
98
2.2
18
1.3
200
1.1
6.1
1.4
0.38
0.21
3.6
1.8
500
0.44
0.22
1000
0.73
Screw: W26x0.706
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18
MICROSCOPE SYSTEM BXFM
BXFM frame
BXFM-F
Widely used system that allows use in combination with fiber illumination, motorized revolving nosepiece and telan lens unit.
Can easily be integrated into other equipment. Attach to the equipment by rear bolt mounting screw or pillar mounting hole.
84
36
82
58.5
4–M4 depth9
36
4-M8 depth8
(Bolt mount screw)
35
4–M4 depth7
66.2
Pillar mount hole center
2A00002
98
110
Weight: 1.9kg
Unit: mm
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19
MICROSCOPE SYSTEM BXFM
BXFM
BXFM-F+BXFM-ILH+BXFM-ILHSPU
Accommodates the reflected light brightfield/darkfield and fluorescence illuminators.
Light axis
165
Pillar axis
130
Holder mounting position
83
Light axis
Revolving nosepiece mounting position
Objective lens
mounting position
Specimen position
Weight: 3.2kg
Unit: mm
BXFM combination sample
BXFM-F+BXFM-ILH+BXFM-ILHSPU+TR30-2+BX-RLA2+U-LH100L-3
165
130
87.5
83
ø 32
Specimen
surface
220
180
587
* For installation dimensions, refer to those for the BXFM-F (page 19).
Weight: 8.2kg (exclude objective lens)
Unit: mm
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20
MICROSCOPE SYSTEM BXFM
BXFM-S
BXFM-F+BXFM-ILHS
Compact focusing unit suitable for building into existing equipment.
141
106
Pillar axis
Holder mounting position
Light axis
59
Revolving nosepiece
mounting position
Objective lens
mounting position
Weight: 2.4kg
Unit: mm
Specimen position
BXFM-S combination sample
BXFM-F+BXFM-ILHS+TR30-2+U-KMAS+U-LH100L-3
290
106
187
ø32
19-49
(Stroke)
Specimen surface
84
208
* For installation dimensions, refer to those for the BXFM-F (page 19).
Weight: 5.5kg (exclude objective lens)
Unit: mm
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21
MICROSCOPE SYSTEM BXFM
Stands
A wide variety of stands are available to suit different applications and purposes.
SZ2-STU2
Universal stand type 2
Major specifications
Item
Specifications
1 Diameter of focusing arm or
fixing section of tube
ø32mm
2 Vertical pole diameter
3 Horizontal poles diameters
ø40mm
ø25mm
(both upper and lower poles)
Horizontal: 234mm,
4 Stroke
Vertical: 205mm
5 Movement range
Horizontal: 541 (435+106) mm max.
(Vertical pole —
300
350
BXFM-S optical axis)
6 Maximum specimen weight Forward: 10kg
(within 90-degree area)
622
214~435
Transverse direction: 6kg
Backward direction: 7kg
(at maximum stroke)
30kg
7 Weight
Ø32
ø40
130
* The rotation angle of the horizontal arm can restrict to 90 degrees with stopper.
U-ST
Compact stand
SZ-STL
Large stand
400
35
106
ø32
73
143
ø32
Weight: 1.8kg
Weight: 5kg
Unit: mm
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22
ILLUMINATION UNITS
Reflected light illuminator for BF/DF
BX-RLA2
ND filters are linked when exchanging between brightfield and darkfield.
144
31.5
Accessories
Unit name
U-25LBD
U-25IF550
U-25ND6
U-25ND25
U-25FR
U-25L42
U-PO3
U-POTP3
Description
LBD filter slider
IF550 filter slider
ND filter
ND filter
Frost filter slider
UV-cut filter
Polarizer slider for reflected light
Polarizer slider for reflected light
with tint plate
Weight (g)
20
20
20
20
20
20
71
71
7.5
U-AN360-3
U-AN
U-DICR
U-DICRH
360° rotatable analyzer slider
Analyzer slider for reflected light
DIC slider for reflected light
DIC slider for reflected light
(high resolution type)
DIC slider for reflected light
(high contrast type)
79
50
130
130
38.7
29.4
56.8
U-DICRHC
130
Revolving
nosepiece
mounting
position
Illuminator
mounting
position
45
265
335
Weight: 3.4kg
Universal reflected light illuminator
BX-URA2
Suitable for observations ranging from brightfield to fluorescence.
Six mirror units can be attached to this reflected light illuminator simultaneously.
Accessories
Unit name
U-25LBD
U-25IF550
U-25ND6
U-25ND25
U-25FR
U-25L42
U-PO3
U-POTP3
Description
LBD filter slider
IF550 filter slider
ND filter
ND filter
Frost filter slider
UV-cut filter
Polarizer slider for reflected light
Polarizer slider for reflected light
with tint plate
Weight (g)
20
20
20
20
20
20
71
71
U-AN360-3
U-AN
U-DICR
U-DICRH
360° rotatable analyzer slider
Analyzer slider for reflected light
DIC slider for reflected light
DIC slider for reflected light
(high resolution type)
79
50
130
130
86.8
27.2
U-DICRHC
DIC slider for reflected light
(high contrast type)
130
Revolving
nosepiece
mounting
position
U-MBF3
U-MDF3*
U-MDIC3
U-MBFL3
Mirror unit for reflected brightfield
Mirror unit for reflected darkfield
Mirror unit for reflected DIC
Mirror unit for reflected brightfield,
for high intensity light source
Fluorescence mirror unit for
reflected (U excitation)
Fluorescence mirror unit for
reflected (B excitation)
Fluorescence mirror unit for
reflected (G excitation)
80
80
80
80
41
Illuminator
mounting
position
261
U-MWUS3
U-MWBS3
U-MWGS3
80
80
80
367
Weight: 3.8kg
Unit: mm
* U-RCV (DF converter for BX-URA2) is needed with darkfield observation.
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23
ILLUMINATION UNITS
Reflected light illuminators for BF
BX-KMA/BX-KMA-ESD
Enables brightfield, Nomarski DIC and simple polarizing observations. ESD model is also available.
Accessories
Unit name
U-25LBD
U-25IF550
U-25ND6
U-25ND25
U-25FR
U-25L42
U-PO3
U-POTP3
Description
LBD filter slider
IF550 filter slider
ND filter
ND filter
Frost filter slider
UV-cut filter
Polarizer slider for reflected light
Polarizer slider for reflected light
with tint plate
Weight (g)
20
20
20
20
20
20
71
71
U-AN360-3
U-AN
U-DICR
U-DICRH
360° rotatable analyzer slider
Analyzer slider for reflected light
DIC slider for reflected light
DIC slider for reflected light
(high resolution type)
DIC slider for reflected light
(high contrast type)
79
50
130
130
Revolving
nosepiece
mounting
position
Cable length 260.5mm
Illuminator
mounting
position
U-DICRHC
130
30
250
312.5
* Combine SZX-TLGAD when using fiber illumination.
Weight: 3.1kg
Unit: mm
MOUNTING DIMENSIONS OF ILLUMINATORS (BX-RLA2, BX-URA2 and BX-KMA/BX-KMA-ESD)
100 (±0.1)
21
170 (Distance to the light axis)
36 (±0.1)
2
5.5
4-M5 depth12 or more
3.5
Location face
Location face
82 (Revolving nosepiece relief dimension)
Convex section(2-4) for positioning
Fix illuminator using four M5 screws and projection for fastening.
Unit: mm
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24
LAMP HOUSING & ACCESSORIES
Reflected light illuminator for BF
U-KMAS
Very compact reflected light illuminator with reduced depth.
Accessories
Unit name
U-25LBD
U-25IF550
U-25ND6
U-25ND25
U-25FR
U-25L42
U-PO3
U-POTP3
Description
LBD filter slider
IF550 filter slider
ND filter
ND filter
Frost filter slider
UV-cut filter
Polarizer slider for reflected light
Polarizer slider for reflected light
with tint plate
Weight (g)
20
20
20
20
20
20
71
71
U-AN360-3
U-AN
U-DICR
U-DICRH
360° rotatable analyzer slider
Analyzer slider for reflected light
DIC slider for reflected light
DIC slider for reflected light
(high resolution type)
DIC slider for reflected light
(high contrast type)
79
50
130
130
21
155
198
Weight: 1.2kg
U-DICRHC
130
Unit: mm
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25
LAMP HOUSING & ACCESSORIES
Lamp housings
Various different lamp housings are available, for use with different light sources: choose to suit the intended purpose.
* If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand.
U-LH75XEAPO
75W xenon apo lamp housing
U-LH100HGAPO
100W mercury apo lamp housing
U-LH100HG
100W mercury lamp housing
(148.5)
30.2
(148.5)
(30.2)
83.5
65
83.5
65
65
93
93
65
8
8
169 (depth dimension for installation)
180.5
130
70
169 (depth dimension for installation)
180.5
130
18.5
Cable length 2,000mm Accepted lamp: UXL-75XB Weight: 3.1kg
Cable length 2,000mm Accepted lamp: USH-103OL
Weight: 2.7kg
*Power supply unit (U-RX-T200) and power cable (UYCP) are necessary for 75W xenon
lamp housing. These items are sold separately.
* Power supply unit (U-RFL-T200) and power cable (UYCP) are necessary for 100W
mercury lamp housings. These items are sold separately.
U-RX-T200 dimensions: 115(W)x195(D)x260(H), weight approx. 3kg
U-RFL-T200 dimensions: 150(W)x295(D)x200(H), weight approx. 4.8kg
U-LH100-3/U-LH100IR/U-LH100L-3
100W halogen lamp housings
Cable length U-LH100-3: 290mm
U-LH100IR: 290mm
Weight: 880g
U-LH100L-3: 800mm
Accepted lamp: 12V100WHAL (high intensity lamp)
12V100WHAL-L (long life lamp)
135(depth dimension for installation)
146.5
* External power supply (TH4-100 or TH4-200) and power cable (UYCP) are necessary for 100W halogen
lamp housings. These items are sold separately. For TH4-100/200 installation dimensions, refer to the next page.
Unit: mm
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26
LAMP HOUSING & ACCESSORIES
Lamp housing accessories
For the 100W halogen lamp, the external power supply TH4-100/200 with an intensity adjustment switch and an ON/OFF switch,
both are located close to the operator's hand, are provided. All Olympus reflected light illuminators can be used with fiber illumination.
* If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand.
TH4-100/200
External power supply
TH4-HS
Hand switch
Cable length:
2,000mm
18.5
Weight: 2.2kg
Weight: 140g
42
38
75
14.5
200
U-RMT
Extension cord
1,700
Weight:200 g
U-RCV
DF converter for BX-URA2
U-LGAD
Fiber adapter for reflected light
observation
SZX-TLGAD
Transmitted light guide adapter
37.5
21
26
24
(dimension
for installation)
44
51
(dimension
for installation)
31.5
(dimensions
for installation)
Darkfield light excluding tube
which is built into the BX-URA2.
62.5
* Mountable with BX-KMA/BX-KMA-ESD only.
(42.5)
Weight: 315g
Light guide mount hole ø12
Weight: 390g
Light guide mount hole ø12
Weight: 135g
Unit: mm
i
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27
LAMP HOUSING & ACCESSORIES
LG-PS2*
Light source
ø15 (Light guide mounting position)
8
76
235
10
251
126
*The types of model varies by country in use.
Weight: 1.6kg
LG-SF
Light guide
Groove: Width3, Depth1
10
31
30
61
25
20
1,000
Weight: 210g
U-DULHA
Double lamp house adapter
82
202
Weight: 1.2kg
Unit: mm
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28
OBSERVATION TUBES
Widefield trinocular observation tubes
Trinocular observation tubes with widefield of view. Compatible with F.N. 22.
U-TR30-2/
Widefield trinocular tube
U-ETR-4
Widefield erect image trinocular tube
U-TR30IR
Widefield trinocular tube for IR
60.6
43.5
163.1
199.9
150.5
186.6 (IR: 188.9)
51.6
Unit: mm
Inclination angle
(degree)
Interpupillary distance
(mm)
Light path selector
Weight
(g)
Field Number
Name
U-TR30-2
Observation image
(F.N.)
22
(eyepiece/video port)
100/0, 20/80, 0/100
100/0, 0/100
30
30
30
50-76
50-76
50-76
Inverted
Inverted
Erect
1600
1600
1900
U-TR30IR
U-ETR-4
22
22
100/0, 0/100
*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm.
Single port tube with lens
When the visual observation is not needed and only video observation is required, a single port tube with a built-in telan lens
can be attached directly to the video port.
U-TLU
Single port tube with lens
U-TLUIR
Single port tube with lens for IR
ø60
Weight: 350g
• For attachable video camera adapters, refer to video camera adapters system diagram page (pages 5-6).
Unit: mm
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29
OBSERVATION TUBES
Super widefield trinocular observation tubes
Trinocular observation tubes with super widefield of view. Compatible with F.N. 26.5.
U-SWTR-3
Super widefield trinocular tube
U-SWETR
Super widefield erect image trinocular tube
68.6
49.9
98.6
62
173.8
220.8
201.9
248.9
MX-SWETTR
Super widefield erect image tilting trinocular tube
318.527
328.338
337.718
72.200
Unit: mm
Field Number
(F.N.)
Inclination angle
(degree)
Interpupillary distance
(mm)
Light path selector
(eyepiece/video port)
Weight
(g)
Name
U-SWTR-3
Observation image
26.5
26.5
26.5
24
24
50-76
50-76
50-76
100/0, 20/80, 0/100
100/0, 0/100
Inverted
Erect
2300
4200
4200
U-SWETR
MX-SWETTR
0-42
100/0, 0/100
Erect
*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm.
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30
INTERMEDIATE TUBES & ACCESSORIES
Intermediate tubes
Various accessories for various observation need.
U-CA
U-ECA
Magnification changer 2x
Magnification changer
Provides 1x, 1.2x, 1.6x and 2x intermediate magnifications.
Provides 1x and 2x intermediate magnifications.
88
96
42
ø75
Weight: 1.3kg
ø70
Weight: 1.3kg
U-TRU
Trinocular intermediate attachment
Intermediate attachment which divides the light path,
allowing attachment of both digital and video cameras.
183.9
106.9
BI:PT=100:0/20:80
Weight: 1.3kg
Unit: mm
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31
INTERMEDIATE TUBES & ACCESSORIES
U-DP
Dual port
U-DP1xC
Dual port 1x
Use this intermediate tube to divide the light path.
Combine with U-DP to obtain a 1x image.
88
17.53
1-32UN
4.5
170.5(mount face)
182
Weight: 500g
51(mount face)
Weight: 1kg
Light path selector by mirror unit
U-APT
Arrow pointer
Transmitted side port: side port = 100:0
Transmitted side port: side port = 70:30 (with use of U-MBF3)
Projects an arrow into the field of view.
U-EPA2
Eyepoint adjuster
Raises eyepoint by 30mm.
88
15V0.2A
0.92 (tolerence from light axis)
120
45.3
Weight: 1.2kg
Weight: approximately 500g
Unit: mm
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32
EYEPIECES/FILAR MICROMETER EYEPIECE
Eyepieces
Eyepieces for UIS2 optical system.
WHN10x
Widefield eyepiece
WHN10x-H
CROSSWHN10x
Widefield eyepieces
WH15x
Widefield eyepiece
SWH10x-H
MICROSWH10x
CROSSSWH10x
Super widefield eyepieces
ø46.2
ø43.2
ø39
ø41
ø41
ø41
ø36.5
ø38.5
ø38.5
EP
EP
ø30
ø30
ø30
Diopter
ø30
Weight
(g)
Field
Micrometer
Name
WHN10x
WHN10x-H
CROSSWHN10x
WH15x
SWH10x-H
MICROSWH10x
CROSSSWH10x
adjustment range
(1/m)
Remarks
Number
diameter (mm)
22
22
22
14
26.5
26.5
26.5
—
24
24
—
24
—
—
—
90
170
170
90
210
210
210
-8 — +5
-8 — +5
—
-8 — +2
-8 — +2
-8 — +2
With adjustable diopter
With cross lines and adjustable diopter
With adjustable diopter
With micrometer and adjustable diopter
With cross lines and adjustable diopter
Unit: mm
*EP=eyepoint
Filar micrometer eyepiece
U-OSM
Used for precise measurement in the field of view.
137
129.2
117.8
23.5
Eyepiece
Magnification 10✕, erect image (inverted when
used with erect image observation tube),
F.N. 14. Diopter adjustment range: 5 1/m.
Provided with rubber eye shade.
Measuring scale Scale lines graduated in increments of 1mm in
the entire 10mm length. Shift of scale lines: 1mm
per rotation of the shift ring, the circumference of
which is divided into 100 graduations.
EP
Measuring range 10mm/objective lens magnification
Compensation
limit for objective
lens magnification
tolerance
5% by combined use of the zoom
Mounting position
(inside)
compensation ring and the provided stage
micrometer. Compensation ring clamping screw.
Magnification compensation scale.
Actual size
Measured value (mm)
Actual size (mm) =
Objective lens magnification
29.5
75.5
Repeatability
0.007
A
Repeatability error
mm
…
(A
Objective lens magnification)
Accuracy
*Measuring error
Weight: 580g
…
…
(A
L
Objective lens magnification:
Measured length in mm)
0.007
[ (0.0002✕A+0.002) L +
] mm
A
Unit: mm
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33
REVOLVING NOSEPIECES
Revolving nosepieces for BF objective lenses
Choose from following 6 types. For motorized nosepieces, refer to motorized unit page.
U-5RE-2
Quintuple revolving nosepiece
U-D6RE
U-D7RE
Sextuple revolving nosepiece with slider slot for DIC
Septuple revolving nosepiece with slider slot for DIC
U-D6RE-ESD
Sextuple revolving nosepiece with slider slot for DIC
with ESD treatment
(87.4)
(125.6)
26.5
60.9
38
87.6
(114.4)
38
76.4
Weight: 520g
Weight: 800g
Weight: 980g
U-P4RE
U-P6RE
Centerable quadruple revolving nosepiece with
slider slot for DIC
Centerable sextuple revolving nosepiece with slider slot for DIC
(125.6)
(114.4)
38
76.4
38
87.6
Weight: 1kg
Weight: 1kg
Insert the DIC dummy when not using the DIC slider
Unit: mm
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34
REVOLVING NOSEPIECES
Revolving nosepieces for BF/DF objective lenses
Choose from following 3 types. Use of adapter to mount BF objectives (BD-M-AD) enables attachment of brightfield objective
lenses. For motorized nosepieces, refer to motorized unit page.
U-5BDRE
U-D5BDRE
Quintuple revolving nosepiece for BF/DF
with slider slot for DIC
U-D6BDRE
Sextuple revolving nosepiece for BF/DF
with slider slot for DIC/
Quintuple revolving nosepiece for BF/DF
U-P5BDRE
Centerable quintuple revolving nosepiece
(125.6)
(114.4)
(111.2)
38
87.6
34.8
76.4
38
76.4
Weight: 800g
Weight: 800g
Weight: 1kg
Insert the DIC dummy when not using the DIC slider
BD-M-AD
Adapter to mount BF objectives
W26✕0.706
W20.32✕0.706
Weight: 10g
(4)
4
Unit: mm
8
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35
VIDEO CAMERA ADAPTERS
C-mount video camera ports
Allows direct attachment of a C-mount video camera. Four types are provided: 0.63x, 0.5x, 0.35x and 0.25x.
All models feature a focus adjustment function
U-TV0.25xC
C-mount video port with 0.25x lens
U-TV0.35xC-2
C-mount video port with 0.35x lens
1-32UN
Image plane
ø36
1-32UN
Image plane
ø60
ø60
ø64
Weight: 1.2kg
Weight: 100g
U-TV0.5xC-3
C-mount video port with 0.5x lens
U-TV0.63xC
C-mount video port with 0.63x lens
ø30
1-32UN
Image plane
1-32UN
Image plane
ø30
2
A
0
0
0
0
1
ø60
ø60
Weight: 200g
Weight: 430g
Unit: mm
Field of view (F.N.)
Projection area (F.N.)
]Video camera adapter
(Projection lens)
Projection
magnifications
2/3" CCD
1/2" CCD
2/3" CCD
1/2" CCD
1/3" CCD
U-TV1x-2
1x
11
17.5
22
8
12.7
16
6
9.5
12
U-TV0.63xC
U-TV0.5xC-3
U-TV0.35xC-2
U-TV0.25xC
0.63x
0.5x
0.35x
0.25x
—
22
17.1
24
—
—
Projection area (Field Number)
Objective lens magnifications
Practical field of view (mm) =
Projection area
Focus the video camera adapter to prevent defocusing the eyepiece image and defocusing by magnification switching.
Generally, the video camera adapter is focused by switching to a low magnification after focusing at a high magnification.objective lens.
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36
VIDEO CAMERA ADAPTERS
Video camera mount adapters
Allows attachment to video cameras with C, Bayonet, Sony and F mounts.
Use with the U-TV1x-2. Focus by amount of screwing into U-TV1x-2.
U-CMAD3
U-BMAD
U-SMAD
C-mount adapter
Bayonet mount adapter
Sony mount adapter
ø44.5
ø30
1-32UN
Image
plane
Image plane
Image plane
ø48
ø42
M56X2
M56X2
M56X2
ø64
ø64
ø64.4
Weight: 165g
Weight: 80g
Weight: 90g
U-TMAD
U-FMT
T mount adapter
F/T mount adapter *
Image plane
Image plane
M42X0.75
ø42
ø45.7
ø54.7
M56X2
ø64
Weight: 70g
Weight: 30g
* It must be combined with U-TMAD
Unit: mm
Video camera port
This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens.
U-TV1x-2
Video port 1x
ø64
ø60
Weight: 150g
Unit: mm
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37
MOTORIZED UNITS
Motorized units
Various motorized units, perfect for automation of equipment, are available.
BX-RLAA+U-D6REMC+U-LH100-3
Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen lamp housing
Enables motorized exchange of objective lenses, selection between brightfield and darkfield observations as well as aperture diaphragm
closing/opening. The BX-UCB control unit has an RS232C connector, allowing control via a PC.
For method of attaching illuminator, refer to page 24.
86.2
(487)
87
400
Illuminator cable length: 1.800mm Weight: 5.5kg(exclude objective lens)
BX-RFAA
Motorized universal reflected light illuminator
Reflected light fluorescence illuminator with simultaneous attachment of six mirror units. Incorporates motorized mirror unit changeover and shutter.
86.8
27.2
Revolving
nosepiece
mounting
position
Illuminator
mounting
position
Illuminator cable length: 1.800mm Weight: 4.3kg
Unit: mm
135
41
261
371
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38
MOTORIZED UNITS
U-D6BDREMC
Motorized sextuple BD revolving nosepiece with
slider slot for DIC
U-D5BDREMC
Motorized quintuple BD revolving nosepiece with
slider slot for DIC
U-P5BDREMC
Motorized centerable quintuple BD revolving nosepiece with
slider slot for DIC
U-D6REMC
Motorized sextuple revolving nosepiece with
slider slot for DIC
U-P5REMC*
Motorized centerable quintuple revolving nosepiece with
slider slot for DIC
74.5
83.5
103.5
103.5
135
135
150
40.5
150
40.5
Weight: 1.4 kg
*Please contact Olympus for the availability. Weight: 1.1kg
BX-UCB
Control unit
U-HSTR2
Hand switch
Motorized units including motorized illuminator and auto focus unit can be totally controlled
from BX-UCB
32
7°
146
Cable length 2000mm
Weight: 370g
310
125
332 (depth)
Weight: 1.0kg
* Extension cord U-RMT (1700mm) should be used to connect the lamp housing (U-LH100-3) to the BX-UCB.
BX-REMCB
U-ACAD4515
AC adapter for BX-REMCB
Control box for motorized nosepiece and BF/DF illuminator
+100
BX-RLAA and U-D5BDREMC/U-D6REMC/U-P5REMC can be controlled from
U-HSTR2, or direct from the computer keyboard via an RS232C connector.
2000
0
* BX-RFAA and U-D5BDREM/U-D6REM combination not applicable.
39.8
34
190.4
129.5±1
Unit: mm
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39
MOTORIZED UNITS
U-AFA2M-VIS
Active auto focus unit
Featuring an AF laser light source in wavelength 785 nm. The multiple-spots sensor enables the high-speed and stable focusing of specimens with
variable height differences.
313
62.5
Weight: 2.6kg
Cable
AFA2M-CBL2M Weight: 0.23kg length: 2m
AFA2M-CBL3M Weight: 0.36kg length: 3m
* Consult your Olympus dealer about the motorized focus.
U-FWR
Motorized reflected filter wheel
Accomplish maximum 6 filter position exchange
180.5
147.9
Weight: 1.0kg
2000
Unit: mm
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40
MOTORIZED UNITS
BXFMA-F
Motorized illumination with power focus
A motorized microscope unit for integration with your equipment. Motorized operations such as revolving nosepiece up/down, objective lens
switching, aperture diaphragm open/close, and brightfield/darkfield switching are accomplished with this component.
Several microscopic operations are totally controlled from an external unit by combining this component with an auto focus unit.
This is the configuration combined with Active Auto Focus Unit U-AFA2M-VIS, Single Port Tube with Lens U-TLU, a lamphousing, a motorized
nosepiece and objective lenses.
32.5
Stroke
Specimen surface
77
51
(standard focusing position)
334
342.1
92
* Consult your Olympus dealer about the mounting dimensions.
Weight: 13kg
(BXFMA-F frame 7.6kg)
U-FH
Focus adjustment knob unit
U-IFFH
Focus adjustment knob interface
70
33.6
Weight: 760g
210
214
70
75.5
4
54
82.3
Weight: 1450g
Unit: mm
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41
DEEP ULTRAVIOLET OBSERVATION SYSTEM
Deep ultraviolet observation system
This module adds a deep ultraviolet (248nm) optical system to a general microscope.
An ultra-high resolution observation is executed by using an extremely short wavelength ray.
U-UVF248IM
UV248 compatible intermediate tube
U-UVF2FB/5FB
UV quartz light guide
+200
0
+200
or 5000
0
2000
79
Weight: U-UVF2FB 50g
U-UVF5FB 80g
105.8
258.5
ø39.4
1-32 UNF
(C Mount Thread)
ø30
196.8
ø70
196
Weight: 1.9kg
U-UVF248LB+U-LH80HGXE
UV248 compatible light source box + Mercury Xenon lamp housing
45
30
150
180
4-ø4.5, ø8 C'bore 5 Deep
4-ø4.5, ø13 C'bore 5 Deep
240
41
Weight:6.5kg
Unit: mm
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42
OPTICAL TERMINOLOGY
1. Field Number (F.N.) and Practical Field of View
5. Total Magnification
The field number (F.N.) is referred to as the diaphragm size of
eyepiece in mm unit which defines the image area of specimen.
The diaphragm diameter actually seen through eyepiece is
known as the practical field of view (F.O.V.) which is determined
by the formula:
5.1 Observation through eyepiece (binocular observation)
M(ob)×M(oc)
M(bino)=
M(bino): Total magnification for binocular observation
M(ob): Objective lens magnification
M(oc): Eyepiece magnification
Eyepiece F.N.
F.O.V. =
(mm)
Objective lens magnification
5.2 Video monitor observation
● Total magnification for video monitor
M(ob)×M(video camera adapter
M(video monitor)=
)×Monitor magnification*
2. Working Distance (W.D.)
M(video monitor): Total magnification on the video monitor
M(ob): Objective lens magnification
The distance between the front edge of the objective lens and
the specimen surface (with the surface of the cover glass in case
of the cover glass objective lens) when the specimen is focused.
M(video camera adapter): Projected magnification for video camera
adapter including photo eyepiece
(refer to Figure 1)
* Refer to Figure 3 for "Monitor magnification"
● Practical field of view for video monitor observation
3. Parfocal Distance
Image device size *
Practical field of view for
video monitor observation
=
It is the distance between the objective lens mounting plane and
the specimen. In UIS2/UIS objective lenses, the parfocal
distance is designed at 45mm.
M
(ob)×M(video camera adapter)
M(ob): Objective lens magnification
M(video camera adapter): Projected magnification for video camera
adapter including photo eyepiece
Working distance and parfocal distance
Objective lens
mounting position
(refer to Figure 1 for projected magnifications)
* Refer to Figure 2 for image device size
Figure 1 Video camera adapter and projection magnifications
Video camera adapter (Projection lens)
U-TV1x-1 +
Projection magnifications
1x
Parfocal distance
video camera mount adapters
Working Distance (W.D.)
U-TV0.63xC
U-TV0.5xC-3
U-TV0.35xC-2
U-TV0.25xC
0.63x
0.5x
0.35x
0.25x
Focal plane
Figure 2 Imaging device size
Camera format
Diagonal
6.0mm
8.0mm
Horizontal
4.8mm
6.4mm
Vertical
3.6mm
4.8mm
6.6mm
For parfocal distance of the LCPLFLN-LCD series objective
lenses, refer to the appropriate objective lens page.
1/3"
1/2"
2/3"
11.0mm
8.8mm
The above table is for standard image device sizes.
Check your device size for precise calculation.
4. Relationship between the objective lens's focal length
and magnifications
Figure 3 Imaging device size and monitor magnifications
Monitor size (diagonal)
Camera format
10"
15"
17"
19"
21"
Indicated magnifications of UIS2/UIS objective lenses are the
values when the focal length of the tube lens is 180 mm.
1/3"
1/2"
2/3"
42.3x
31.8x
23.1x
63.5x
47.6x
34.6x
72.0x
54.0x
39.3x
80.4x
60.3x
43.9x
88.9x
66.7x
48.5x
Focal length of tube lens
M(ob)=
f
Example
What is total magnifications for video monitor when objective
lens is 50x, video camera adapter U-TV0.5xC, 2/3" video
camera and 21" monitor are used ?
M(ob): Objective lens magnification
f: Objective lens's focal length
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43
OPTICAL TERMINOLOGY
•Total magnification on the video monitor:
● Resolving power formula
m
(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and monitor
The following formula is generally used for determing resolution.
magnification is 48.5× from Figure 3.
λ
N.A.
(
ε = 0.61 ×
Reyleigh formula)
M
(monitor observation)=M(ob)×M(video camera adapter)×monitor magnification
=50×0.5×48.5=1213×
λ: Wavelength or radiation in use
(λ=0.55µm is used for visible light)
N.A.: Objective lens N.A.
•Practical filed of view for video observation(horizontal side):
M
(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and
horizontal side of 2/3" imaging device is 8.8mm from Figure 2
Example
MPLFLN100×(N.A.=0.90), λ=0.55µm
Image device size
Practical field of view
for video observation
=
M
(ob) × M(video camera adapter)
λ
0.3355
N.A.
0.3355
0.90
ε = 0.61 ×
=
=
= 0.37µm
8.8 (mm)
50 × 0.5
N.A.
=
=352µm
8. Focal depth of Microscope
6. Numerical Aperture (N.A.)
The focal depth refers to the depth of the specimen layer which
is in sharp focus at the same time, even if the distance between
the objective lens and the specimen plane is changed when
observing and shooting the specimen plane by microscope. As
human eyes are individually different in the ability of their focus
adjustment, each person's perception of the focal depth varies.
At present, the Berek formula is generally used, because it gives
a focal depth value that often coincides with that obtained
through experiments.
The numerical aperture is a key factor to the performance of
objective lens (resolving power, focal depth and brightness).
The N.A. is determined by the following formula:
N.A.= n × sinθ
n=Refraction rate of the medium between specimen and
objective lenses. (Air: n=1, oil: n=1.515)
θ: Angle which is made by the optical axis and refraction of the
light farthest from the center of the lens.
Focal depth formula
The visual field brightness (B) of the microscope is determined
by the following formula in relation to the objective lens
magnification (M). The larger the N.A. and the lower the
objective magnification, brightness will increase in the factor of
the second power.
● Visual observation (Berek formula)
ω×250,000
N.A. × M
λ
(µm)
D.O.F.=
+
2
2(N.A.)
D.O.F.: Depth Of Focus
2
N.A.
M
ω: Resolving power of eyes 0.0014
B ∝
2
(when optical angle is 0.5 degrees)
M: Total magnification
Numerical aperture
(objective lens magnification x eyepiece magnification)
Objective
350
N.A. × M
0.275
N.A.
D.O.F. =
+
(λ=0.55µm)
➔
2
n=1
(air)
θ
This indicates that the focal depth becomes smaller as the
numerical aperture becomes larger.
Sample
surface
Example
With MPLFLN100×(N.A.=0.90), WHN10×:
350
0.90 × 1,000
0.275
0.81
D.O.F. =
+
= 0.39 + 0.34 = 0.73µm
7. Resolving Power
The resolving power of an objective lens is measured by its
ability to differentiate two lines or points in an object. The greater
the resolving power, the smaller the minimum distance between
two lines or points that can still be distinguished. The larger the
N.A., the higher the resolving power.
● Video camera
In the case of a video camera, the focal depth will vary according
to number of pixels of CCD, optical magnification, and numerical
aperture. The above-mentioned formula is used as a rough
guide only.
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44
OPTICAL TERMINOLOGY
9. Aberrations
characteristics of glass materials used for the optical system.
“Expansion of a point image” can also be expressed by
“wavefront aberration” that regards the light as “waves” and
takes account of the phase to include the influence of diffraction.
A difference between an ideal image and an actual image that
passes through an optical system is called an “aberration.”
9.1 Requirements for Ideal Image Formation
The following three requirements must be satisfied to form an
image with no aberration, or an ideal image.
(i) All the light rays coming from a single point and passing
through an image formation optical system converge on a
single point.
(1) Spherical aberration
When light rays coming out of an axial object point enter a lens,
the light rays with a larger numerical aperture (N.A.) are
subjected to stronger refraction power and cross the optical axis
in positions with larger differences from the ideal image
formation position. The aberration caused this way by different
image forming positions due to differences in N.A. of axial light
rays is called “spherical aberration.” (“Spherical aberration” is
proportional to the cube of N.A.)
(ii) Image points, which correspond to object points on the
same plane perpendicular to the optical axis, are present
on the same plane.
(iii) The planar shape of an object and the planar shape of an
image that are on the same plane perpendicular to the
optical axis have a similarity relation.
Figure 9-3 Spherical Aberration
Figure 9-1 Requirements for Ideal Image Formation
Specimen
Aplanatic
tube lens
（i）
Image plane
（ii）
（iii）
Object
Objective lens with
spherical aberration
Image plane
It is said that objective lenses with larger N.A. have better
resolution but worsen spherical aberration. Our advanced
design and manufacturing techniques have realized good optical
performance even with large numerical aperture.
In an actual optical system, however, it is very difficult to strictly
meet the requirements for ideal image formation and this causes
“aberrations” that interfere with image forming performance.
(2) Coma aberration
9.2 Classification of Aberrations
Aberrations that interfere with image forming performance are
classified as shown below in Figure 9-2.
Even though spherical aberration is compensated to be very
small, there are cases where light rays coming out of an off-axis
object point are not condensed to a single point on the image
plane but generate asymmetric blur just like a comet leaving
traces. This is called coma aberration.
Seidel’s aberration = “Expansion of a point image” +
“Curvature of image plane” + “Deformation”
Figure 9-4 Coma Aberration and Spot Shape
on the Image Plane
Figure 9-2 Classification of Aberrations
(1) Spherical aberration
Specimen
Aplanatic
tube lens
(2) Coma aberration
Seidel's
(3) Astigmatism
aberration
(4) Field curvature
(5) Distortion
Aberration
Objective lens with
coma aberration
Image plane
(6) Longitudinal (axial)
chromatic aberration
Chromatic
aberration
(7) Chromatic aberration
of magnification
(3) Astigmatism
Even though a lens is compensated for spherical aberration and
coma aberration, there are cases where an image of an off-axis
object point is not focused to a single point but separated to a
concentric line image and a radial line image. This is called
“astigmatism.” When astigmatism is present, a point image
blurs vertically and horizontally, before and after the focus
position.
Types (1) to (3) correspond to “expansion of a point image” that
goes against requirement (i) for ideal image formation in Figure 9-
1. Type (4) corresponds to “curvature of image plane” that goes
against requirement (ii) in Figure 9-1. Type (5) corresponds to
“deformation” that goes against requirement (iii) in Figure 9-1.
Types (6) and (7) correspond to “color blur” of images caused by
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45
OPTICAL TERMINOLOGY
aberration of magnitude.”
Figure 9-5 Astigmatism and Change in Spot
Shape in Different Focus Positions
Many special glass materials are used, e.g., for apochromats
(MPlanApo in Olympus), to eliminate chromatic aberration in a
wide range from violet light (g-rays with wavelength of 435 nm)
to red light (c-rays with wavelength of 656 nm).
9.3 Wavefront Aberration
Since a long time ago, aberrations have been used in “geometric
optics,” which considers light as “light rays.” Microscope optical
systems are often used for observation of very small specimens
at a wavelength level, and sometimes adopt “wave optics,”
which regards light as “waves” and handles the phase
information, taking account of the influence of diffraction.
In such a case, “wavefront aberration” is used for evaluation.
As shown below, when requirements for ideal imaging are
satisfied in a microscope optical system, the spherical wavefront
(spherical waves) coming from a single point on an object
(specimen) is converted to plane waves through an ideal
objective lens. The plane waves are converted to spherical
waves through an ideal tube lens, and condensed to a single
point. The wavefront of these waves is called the “ideal
wavefront.”
(a) (b) (c)
(a)
(b)
(c)
(4) Field curvature
An image plane of an object on a plane perpendicular to an
optical axis does not always become a plane perpendicular to
the optical axis, but it generally becomes a curved plane. This
symptom is called “field curvature.”
When field curvature is present, the image is more displaced as
it becomes closer to the periphery of the visual field. Therefore,
when the center of an image is brought into focus, blur occurs in
the peripheral areas of the image. To bring the entire image,
including the periphery, into clear focus, it is necessary to
adequately compensate for this type of aberration.
Figure 9-7 Ideal Microscope Optical System
Specimen Ideal
objective
lens
Ideal
tube lens
Image plane
(5) Distortion
When there is no similar relation between a planar shape on an
object and a shape on the image plane, this is called “distortion.”
When distortion is present, a square image appears in a shape
of a barrel or pin-cushion as shown in Figure 9-6.
Spherical
wave
Plane
wave
Spherical
wave
Figure 9-6 Distortion
Based on the figure indicated for (1) spherical aberration, the
behavior of the wavefront in an optical system that has an
aberration is described below.
(a) Barrel shape (a) Pin-cushion
type
type
Figure 9-8 Illustration of Wavefront Aberration
Actual
wavefront
Specimen
Ideal
wavefront
The microscope optical system contains some distortion. When
distortion is present, it can bring erroneous results of shape
measurements. When a microscope is used for precision
measurements, pay close attention to this aberration, for
example, by providing it with an aberration compensation
function.
Objective lens with
spherical aberration
(6) Chromatic aberration
A difference (a degree of disagreement) between the ideal
wavefront and the actual wavefront shown above is called
“wavefront aberration.”
Glasses used for optical systems have different refractive
indexes depending on the wavelength. This causes differences
in focal length between wavelengths and generates
displacement of image forming position. This phenomenon is
called “chromatic aberration,” which is sometimes subdivided
into axial displacement on the optical axis, called “axial
chromatic aberration” (or lateral chromatic aberration) and
displacement on the image plane, called “chromatic
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46
OPTICAL TERMINOLOGY
9.4 Strehl ratio
When a point light source is observed with an aberration-free
optical system and an aberrated optical system , the former
concentrates the focal point to a point at the image formation
position. In contrast, the latter fails to produce a focal point,
instead causing a spread in the intensity distribution of the point
image (this is known as “point spread”). The specific
appearance of such a point image (i.e. point spread) is shown in
Fig. 9-9.
Figure 9-9 Appearance of condensed light in
the image plane (point spread)
Aberration-free optical system
Aberrated optical system
With the proportion of light concentrated in the image plane
(intensity of light concentrated in the Airy disk) by an aberration-
free optical system serving as 100%, the proportion of light
concentrated by an aberrated optical system is known as the
Strehl ratio. When graphed, the Strehl ratio reveals peaks in
intensity as shown in Fig. 9-10. The higher the SR, the closer an
optical system is to being aberration-free.
Figure 9-10 Strehl ratio
Intensity
Aberration-free
optical system
100%
Aberrated
optical system
SR
Image plane
A Strehl ratio of 80% is typically called the diffraction limit, and
lenses with a lower ratio lack the performance required to serve
as an objective lens. A ratio of over 95% means that the lens’
performance in general observations is comparable to that of an
aplanatic lens (which is corrected for spherical aberrations and
coma).
Note) A laser interferometer is used for actual assessment of
optical performance, so assessment is done at a single
wavelength. Unless otherwise noted, Strehl ratio measurements
are at the e-line (544nm).
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OLYMPUS CORPORATION has obtained ISO9001/ISO14001
Illumination devices for microscope have suggested lifetimes. Periodic inspections are required.
Visit our Website for details.
Specifications are subject to change without any obligation on the part of the manufacturer.
Printed in Japan M1606E-0408B
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