Leica M125
Leica M165 C
Leica M205 C
Leica M205 A
Manual
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Safety concept
The individual modules of the Leica M stereo-
microscopy series include an interactive
CD-ROM with all relevant user manuals in 20
other languages. Keep it in a safe place, and
readily accessible to the user. User manuals and
updates are also available for you to download
and print from our web site at www.stereomi-
croscopy.com.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please observe all safety instruc-
tions.
This operating manual describes the special
functions of the individual modules of the Leica
M stereomicroscopy series and contains impor-
tant instructions for their operational safety,
maintenance, and accessories.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
The "Safety concept" booklet contains addi-
tional safety information regarding the service
work, requirements and the handling of stereo-
microscope, accessories and electrical accesso-
ries as well as general safety instructions.
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Leica M series
3
Symbols used
Warning of a danger
Danger due to hot surface
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
This symbol indicates especially impor-
tant information that must be read and
•
complied with. Failure to comply can cause the
following:
Important information
★
★
Hazards to personnel
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Functional disturbances or damaged instru-
ments
Action
This symbol refers to actions described in
the text that are to be carried out.
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that, if not observed,
can cause the following:
★
Figures
★
★
Hazards to personnel
(1) Numbers in brackets within the descrip-
tions relate to the figures and the items
within those figures.
Functional disturbances or damaged instru-
ments.
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4
Safety regulations
Description
non-Leica components that are outside of the
scope of this manual.
Requirements for the owner/operator
★ See “Safety concept” booklet
The individual modules fulfill the highest
requirements for observation and documen-
tation of Leica stereo microscopes of the
M series.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Ensure that:
★
The
M
series stereomicroscopes and
accessories are operated, maintained and
repaired by authorized and trained person-
nel only.
Designated use
★
See “Safety concept” booklet
Location of use
★
See “Safety concept” booklet
★
All operators have read, understood and
observe this user manual, and particularly
the safety instructions.
Non-intended use
See “Safety concept” booklet
★
★
Set up the electrical components at least 10
cm from walls and combustible objects.
Never use M series microscopes or their compo-
nents for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
★
★
Avoid large temperature fluctuations,
direct sunlight and vibrations. These condi-
tions can distort measurements and micro-
graphic images.
The devices and accessories described in this
operating manual have been tested for safety
and potential hazards. The responsible Leica
affiliate must be consulted whenever the device
is altered, modified or used in conjunction with
In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build up of
fungus.
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Leica M series
5
Safety regulations (continued)
Repair, service work
Installation in third-party products
See “Safety concept” booklet
Health risks
★
See “Safety concept” booklet
★
Workplaces equipped with stereomicroscopes
facilitate and improve the viewing task, but
they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
★
Only original Leica Microsystems spare
parts may be used.
Disposal
★
See “Safety concept” booklet
★
★
Before opening the instruments, switch off
the power and unplug the power cable.
Legal regulations
Touching the live circuit can cause injury.
★
See “Safety concept” booklet
★
Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
Transport
EC Declaration of Conformity
★ See “Safety concept” booklet
★
Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
★
Thorough training of the personnel, giving
consideration to ergonomic and organiza-
tional aspects.
★
In order to prevent damage from vibrations,
disassemble all moving parts that (accord-
ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
reduce the exertion of the user to a minimum.
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Leica M series
6
Safety regulations (continued)
Direct contact with eyepieces can be a potential
transmission path for bacterial and viral infec-
tions of the eye.
Light sources: safety instructions
Protective measures of the manufacturer:
Warning
UV radiation could damage the eyes. Therefore:
★
UV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
★
★
★
★
Never look into the light spot on the speci-
men plane without a UV protection screen.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Never look into the eyepieces if no excita-
tion filter is in the beam path.
★
Dummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
Fill empty filter positions with dummy filter
carriers (e.g. M165FC).
★
★
UV filters are installed in the observation
beam paths to protect the eyes.
Do not select a white, strongly reflective
background for the stage.
The stray-light protection on the lamp
housing prevents irradiation of the hands.
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Leica M series
7
Safety regulations (continued)
Supply unit
Lamp housing
Mercury lamp
Always unplug the supply unit from the power
supply:
★
★
★
Never open the lamp housing while the
lamp is switched on. Risk of explosion, UV
exposure, blinding!
★
Follow the user manual and safety instruc-
tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.
★
When installing and disassembling the
lamp housing
Before opening the lamp housing, allow it
to cool off for at least 15 minutes. Danger
of explosion!
★
★
Before opening the lamp housing
★
★
For transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
When replacing the high-pressure mercury
lamp and other parts, such as the heat-ab-
sorbing filter or the collector
Never cover the air duct on the lamp hous-
ing. Danger of fire!
★
during maintenance work on the supply
unit.
To minimize the risk of explosion, replace a
discoloured mercury lamp promptly when
it has reached the end of its rated life (follow
the manufacturer's specifications and the
minute meter on the supply unit).
★
Leica Microsystems assumes no liability
for damage caused by exploding, incor-
rectly installed or improperly used mercury
lamps.
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Leica M series
8
Table of Contents
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10
Leica M125 / M165 C
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Leica M series
11
Congratulations!
Congratulations on obtaining your new Leica M series stereomicroscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
Though the reliability and robustness of Leica stereomicroscopes is
legendary, like any high-tech product, the M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
The M series embodies all the qualities you associate with the name Leica
Microsystems: excellent objectives, high-quality engineering, and reli-
ability. Furthermore, the modular design ensures that the M series adapts
perfectly to your needs—no matter which accessories you require for your
tasks.
We wish you the best of success in your work—after all, you are now
equipped with the best tool!
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Leica M series
13
A Step Towards Infinity
Ever since their introduction by Horatio S.
Greenough, stereomicroscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engi-
neers have worked to push magnification, reso-
lution and image fidelity to the limit permitted
by optics.
Limits are made to be broken.
The Leica M205 C is the world's first stereo-
microscope with a zoom range of 20.5:1. This
accomplishment, however, was not enough for
Leica's engineers. With the new FusionOptic™ in
the M205 C, they have succeeded in going yet
another step beyond previous limits. In addition
to the increase in magnification, the resolution,
too, has been increased to up to 1050 lp / mm*,
which corresponds to a resolved structure size
of 472 nm.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimen-
sional image seen by the observer becomes
distorted—a sphere becomes an ellipse, a flat
surface curves toward the observer.
Of course, this performance increase benefits
your everyday work. Set up your specimens
on the microscope with comfortable freedom
of movement and discover details in stereomi-
croscopy that you could never see before.
* Leica M205 C with 1.0× planapochromat and 10× eyepieces
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Leica M series
14
The Electronics: Comfort, Convenience and Safety for your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new
M series. Optics carrier, tripod, base and illu-
minator are all connected using electrical
contacts—which provides a number of advan-
tages.
Reliability for your experiments
diaphragm and more to the Leica LAS software.
Thus you always know the conditions under
which an image was acquired.
Exact reproducibility of results is an increas-
ingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnifica-
tion, the illumination, the position of the iris
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also trans-
ferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables—this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply
the power.
Leica Application Suite (LAS) evaluates the
transmitted data and can restore the test situa-
tion later.
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Leica M series
15
The Modular Design: Everything is Relative
The Leica M series provides maximum flexibil-
ity in choosing equipment, thanks primarily to
the modular configuration and the compatibil-
ity that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.
Despite this, you will notice that the controls
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customer-
specific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.
and individual components do not differ signif-
icantly. Whichever configuration you choose,
you will quickly feel right at home.
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16
Maximum Compatibility
Leica engineers were careful to ensure that the
new M series—like its predecessors—remains
compatible with existing series. This means
that objectives, bases, tubes and so on can be
reused.
Tubes
The interface between the optics carrier and the
tube has remained the same, so existing tubes
fit the new M series. The new tubes are designed
for eyepieces with field number 23, while the
predecessor models were only designed for
field number 21, resulting in a smaller object
field.
Objectives
All new objectives of the M series are parfocal,
meaning that when used with the objective
nosepiece, they can be replaced while keeping
the specimen in perfect focus.
Eyepieces
However, the new M-series eyepieces models
have an audible and tangible click to provide
immediate feedback in case of accidental
adjustment.
If you prefer, you can continue to use the previ-
ous objective series. In this case, parfocality is
no longer guaranteed.
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Leica M series
17
On We Go
If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the instal-
lation instructions and go directly to the Quick
If, on the other hand, you are assembling the
microscope yourself, continue with the "Assem-
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18
Base and Focusing Column
The first step is to connect the focusing column
of the M series to the corresponding base.
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive
2. Securely screw the focusing column to the
base using the six included screws.
Tools used
★
Hex socket screwdriver, 3 mm
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Optics carrier
Tools used
Assembling the optics carrier
★
Hex socket screwdriver, 4 mm
1. Place the optics carrier on the focusing
column so that the screw fit into the thread
provided and the lug fits into the groove.
2. Press the optics carrier backwards to the
focusing column and screw it in place using
your other hand.
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Tube
All intermediate tubes that fit between the
optics carrier and the binocular tube are fitted
in the same manner.
Assembling the tube
2. Push the tube (for example, the inclined
binocular tube) into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.
3. While holding the tube only slightly, care-
fully tighten the positioning screw. It
is automatically brought to the correct
position.
Tools used
★
No tools required.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.
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Eyepieces
Tools used
Preparation
Inserting the eyepieces
★
No tools required.
1. If you want to use an optional graticule,
3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.
Magnification range
You can extend the overall magnification range
using available 10×, 16×, 25× and 40× wide-
field eyepieces for persons wearing glasses.
2. Remove the plastic tube guard.
4. Securely tighten the clamping screws.
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Objective
Tools used
Attaching the objective
Hold the objective firmly during assem-
bly and disassembly so that it does not
fall onto the stage plate. This applies particu-
larly to the 2× planapochromatic objective,
which is very heavy. Remove all specimens from
the stage plate first.
Alternative fastening options
★
No tools required.
★
If using the objective nosepiece, read the
•
Preparation
1. Remove the protective cap on the optics
carrier by turning it.
★
If using the AX carrier, read the instructions
2. Screw the objective clockwise into the
optics carrier.
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Objective Nosepiece – Assembly
Preparations
2. Rotate the moving part by 90° and attach
the objective nosepiece to the drive hous-
ing from the front (!). Screw the objective
nosepiece firmly into place.
4. Screw the optics carrier onto the objective
nosepiece.
Hold the objectives firmly during
•
assembly and disassembly so that they
do not fall onto the stage plate.
5. Screw both objectives onto the objective
nosepiece. It makes no difference which
position an objective occupies.
★
Move the drive housing all the way upwards
and remove the optics carrier, if the carrier
has already been installed.
6. Unscrew the locking screws on both sides
of the objective nosepiece.
Assembly
1. Remove the transport anchor from the
objective nosepiece.
3. Unscrew the three Phillips screws on the
objective mount of the optics carrier and
remove the intermediate ring.
You can now adjust the parfocality (see instruc-
tions on the next page).
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Objective Nosepiece – Adjusting Parfocality
The following procedure only has to be carried
out once. Afterwards, both objectives are parfo-
cal, meaning that the specimen remains in focus
when the objective nosepiece is rotated.
Adjustment
1. Rotate the 2× objective into the beam path
and set it to the lowest magnification.
7. Toggle to the 1× objective.
8. Turn the objective on the thread in both
directions until the specimen appears
absolutely sharp.
2. Focus on the specimen.
This procedure must be repeated if you replace
either of the two objectives with another.
3. Rotate the 1× objective into the beam
path.
By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
with the other objective. If it is not parfocal,
repeat the procedure.
The following example assumes the combi-
nation of the 1× and 2× planapochromats. If
you are using another objective combination,
replace the 2× objective in the description with
the objective with the stronger magnification.
4. Turn the objective on the thread in both
directions until the specimen appears
sharp.
9. Tighten the locking screws.
5. Toggle to the 2× objective.
Preparation
6. Select the strongest magnification and
refocus until the specimen appears abso-
lutely sharp.
★
Open the iris diaphragm.
★
Set the dioptric correction of the eyepieces
to "0".
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26
AX Carrier – Preparation
Tools
★
If the switch position corresponds to the
optics carrier used, skip the following steps.
Otherwise, proceed as follows:
★
Hex socket screwdriver, 2.5 mm
Preparing the AX carrier
2. Unscrew the two screws that hold the
switch in position.
Before the AX carrier is installed, it may first
have to be prepared for the optics carrier to be
used (Leica M125, M205 C or M165 C).
4. Important: push the switch all the way into
the mount while reinserting the screws.
Only this ensures that the switch is posi-
tioned correctly.
1. Check the switch position.
3. Pull out the switch, rotate it into the desired
position and reinsert it.
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AX Carrier – Assembly
Tools
2. Install the AX carrier on the focusing
column.
4. Unscrew the three holding screws and
remove the adapter ring on the optics
carrier.
★
★
Hex socket screwdriver, 4 mm
Philips screwdriver
Once the AX carrier has prepared for the corre-
sponding optics carrier, it can be installed.
1. Move the slide of the AX carrier into mid
position.
3. Screw the objective to the AX carrier.
5. Install the optics carrier on the AX carrier.
Wrong
Right
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Transmitted-light Base TL ST
Unpacking the base
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column to the
bottom using the 4 Allen screws.
The base is delivered with the adapter plate
installed. Make sure the devices are unpacked
on a flat, sufficiently dimensioned, and non-slip
surface.
3. Reattach the adapter plate to its original
position using the 6 Allen screws.
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Transmitted-light Base TL BFDF: Before First Use
Removing the transport anchors
Before you can use the transmitted-
•
light base for the first time, it is abso-
lutely necessary to remove the two transport
anchors as follows.
Anchor of mirror
Anchor of switching slide
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Transmitted-light Base TL BFDF
Standard delivery
The selected stage is mounted on the base
before commissioning. You can switch between
the stages at any time with just a few hand
movements.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
The base is delivered with the adapter plate
installed. The selected stage (IsoPro™ cross-
stage or standard stage 10 447 269), and the
focusing drive will have to be mounted later.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Stage assembly
The TL BFDF transmitted-light base can be
equipped with three different stages.
3. Attach the stage to the base using the four
Allen screws provided.
★
★
★
Leica IsoPro™ manual cross-stage
Leica IsoPro™ automatic cross-stage
Standard stage 10 447 269
4. Insert the glass plate back into the standard
stage.
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TL RC™ / TL RCI™
The base is delivered with the adapter plate
installed. The selected stage (IsoPro™ cross-
stage or standard stage 10 447 269), and the
focusing drive will have to be mounted later.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
Ensure that the instruments are unpacked on a
level, adequately sized, and nonskid underlay.
Stage assembly
3. Attach the stage to the base using the four
Allen screws provided.
The transmitted-light base TL RC™/ RCI™ can
be equipped with three different stages. The
selected stage is mounted on the base before
commissioning. You can switch between the
stages at any time with just a few hand move-
ments.
4. Insert the glass plate back into the standard
stage.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
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IsoPro™ Manual Cross-stage: Assembly
IsoPro™ Cross-stage
Before the IsoPro™ cross-stage is mounted to
Left or right operation
Before the IsoPro™ cross-stage is mounted to
the base, the axis containing the control buttons
is attached either on the left or the right side of
the cross-stage.
the base, the axis containing the control buttons
is attached either on the left or the right side of
the cross-stage.
If the controls are to be mounted on the left-
hand side, the gear rod on the bottom side of
the cross-stage must be unscrewed and reat-
tached in reverse.
If the controls are to be mounted on the left-
hand side, the gear rod on the bottom side of
the cross-stage must be unscrewed and reat-
tached in reverse.
1. Take the glass plate from the cross-stage.
2. Turn the cross-stage around and place it
onto a non-slip surface.
3. Change the gear rod from the left to the
right-hand side.
4. Skip the next two steps to mount the
controls.
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IsoPro™ Manual Cross-stage: Assembly (continued)
1. Take the glass plate from the cross-stage
and turn it around.
Control assembly
1. Take the glass plate from the cross-stage
and turn it around.
Cross-stage assembly
1. Place the cross-stage on the base.
2. Change the gear rod from the left to the
right-hand side.
2. Pull the upper part of the cross-stage care-
fully toward the user, fastening the lower
part onto the transmitted-light base.
2. Attach the axis with the control buttons to
the desired side. The fastener snaps into the
cross-stage magnetically.
4. Attach the axis with the two Allen screws
provided.
5. Attach the cover rail to the cross-stage.
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IsoPro™ Manual Cross-stage: Assembly (continued)
3. Screw the cross-stage evenly onto the three
threaded holes.
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
4. Push the cross-stage all the way back
towards the column.
2. Attach your focusing drive column to the
bottom using the three Allen screws.
5. Reinsert the glass plate back into the stan-
dard stage.
3. Reattach the adapter plate to its original
position using the three Allen screws.
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IsoPro™ Motorized Cross-stage: Assembly
Basics
1. Unpack the cross-stage from the transport
2. Secure the motorized cross-stage to the
base using 3 M4 screws.
The transmitted light bases of the TL series
(TL BFDF, TL RC™, TL RCI™) are supplied with
an installed extension plate. The selected
stage (IsoPro™ cross-stage or standard stage
10 447 269), and the focusing drive will have to
be mounted later.
packaging and position it on the transmit-
ted-light base.
1
The motorized cross-stage is a sensitive preci-
sion instrument. During installation, avoid
subjecting the stage to impact or severe vibra-
tions.
2
1
2
Microscope base
Motorized cross-stage
3× M4 screws
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IsoPro™ Motorized Cross-stage: Assembly (continued)
3. Remove the two M3 screws and the holder
from the cross-stage.
4. Remove the two M4 screws and the sleeve
from the cross-stage.
5. Remove the four shock-absorbing cartons
from the cross-stage.
After removal, keep all the trans-
port anchors in the plastic bag
for future transport.
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Leica M series
37
IsoPro™ Motorized Cross-stage: Assembly (continued)
The bases
Cross-stage and base
Never move the sledge of the motorized
cross-stage manually in the X direction,
as otherwise the mechanical system will be
damaged!
The TL BFDF, TL RC™ and RCI™ transmitted light
bases can be equipped with three different
stages: standard stage, manual and IsoPro™
automated cross-stage. The selected stage is
mounted on the base before commissioning.
You can switch between the stages at any time
with just a few hand movements.
•
1. Place the cross-stage on the base.
2. Pull the upper part of the cross-stage care-
fully toward the user, fastening the lower
part onto the transmitted-light base.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
3. Screw the cross-stage evenly onto the three
threaded holes.
4. Push the cross-stage all the way back
towards the column.
5. Reinsert the glass plate back into the stan-
dard stage.
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38
IsoPro™ Motorized Cross-stage: Assembly (continued)
Cross-stage for X-Y Stage Control Module
1. Plug the CTL2 plug of the cross-stage motor
into one of the three available CTL2 inter-
faces.
As soon as all instruments have been plugged
into the control box and it has been plugged
into the power supply, the cross-stage initializes
and automatically drives to the mid position.
2. Plug the CTL2 plug of the Leica PSC control-
ler into another CTL2 interface.
4. Connect the PC and X-Y Stage DCI Module
(with a suitable USB cable).
★
★
The third CTL2 interface is available for
connecting other instruments, such as the
Leica foot switch (10 447 398) or TL RCI™
transmitted light base (10 446 352).
The 15-pin Sub-D interface is intended for
use with the Leica SmartMove™ controller
(11 501 197).
3. Plug a power cable into the socket provided
and into a grounded power socket.
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Leica M series
39
Cables: Terminals
The new Leica M-series features extensive
encoding with which various microscope data
and settings can be read out, transferred to the
PC and reproduced later.
The Terminals
The connection to the PC and to other instru-
ments is made using the terminals on the rear
side of the column:
1. 2×CTL2 terminals for auxiliary equipment
such as the ring illuminator, the base and
other accessories from the Leica product
range.
1
★
The interface to the optics carrier is on the
column.
3
2
2. Terminal for the 50-watt power supply
provided.
3. USB terminal for the connection to the PC.
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Leica M series
40
Cables: Cable Duct
The integrated cable duct in the column enables
a neat cable layout around the microscope.
For example, the USB or FireWire cables of the
camera can be stowed in the cable duct.
Feeding the cables
1. Unscrew the three screws on the cable
duct.
3. Place the cables in the cable duct and screw
the cover on tightly.
Tip: Estimate the length of the cable ends you
will need before screwing on the cover. For
thick cables, it is difficult to change the length
retroactively.
2. Remove the cover of the cable duct.
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Leica M series
41
Cables: Diagram
Alternative installation
Please use the Leica Application
Suite to configure the functions
of the system.
LED5000 RL
LED5000 MCI™
115/230 V
115/230 V
DCI Controlbox
TL RCI™
PC USB
Power/USB
115/230 V
Footswitch 2...
Footswitch 1
(Connect to free CTL2-port on last footswitch.)
Smart Move
Connector from Cross Stage
PSC
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Leica M series
42
Leica LED5000 MCI™
The Leica LED5000 MCI™ (for "Multi Contrast
Illumination") is installed using two screws. For
optimum accessibility, the optics carrier should
Assembly
1. Hold the LED5000 MCI™ with one
hand and tightly screw the retraining
stirrups on both top holes on the drive hous-
ing.
2. Connect the CAN-bus cable to either of the
two sockets. (The flat part of the plug must
be facing downwards.)
•
3. Plug the other end of the cable into one of
the two "CTL2" sockets on the column.
Constraints
The Leica LED5000 MCI™ cannot be used
together with the objective nosepiece.
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Leica M series
43
Leica LED5000 MCI™: Alternative Assembly
Under certain circumstances, the light source
must not be moved along with the optics
carrier. A typical example is multifocus images
in which the Z-stack changes while the angle
of incidence of the light must remain the same.
For such purposes, the Leica LED5000 MCI™ is
directly fastened to the column.
2. Screw the retaining stirrup into the column
3. Push the Leica LED5000 MCI™ onto the
retaining stirrup.
at the notch using the single screw. If you
tighten the screw, the retaining stirrup is
automatically moved into the correct posi-
tion.
Installation on the column
1. Pull the retaining stirrup out of the Leica
LED5000 MCI™.
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Leica M series
44
Leica LED5000 RL
The ring illuminator, the Leica LED5000 RL
("Ring Light"), is installed on the objective using
a single screw. It has been optimized for a work-
ing distance between 60 and 70 mm.
Assembly
1. Connect the CAN-bus cable to the
ring illuminator. The flat part of the plug
must be facing upwards.
3. Plug the other end of the cable into
one of the two "CTL2" sockets on the
column.
•
•
2. Push the ring illuminator over the
objective as far as it will go and screw it
into place.
For optimum accessibility of the specimen,
the ring illuminator should be installed with
the cable facing backwards. However, it is also
possible to turn the ring illuminator sideways,
for example if simultaneously using the Leica
LED5000 MCI™ system illumination. In this
case, the ring illuminator cannot be connected
directly to the Leica LED5000 MCI™.
Constraints
•
The Leica LED5000 RL can be used in conjunc-
tion with the planapochromat 1× and planapo-
chromat 0.63× objectives. With all other
objectives, the working distance is too low for
adequate illumination.
The ring illuminator cannot be used together
with the objective nosepiece.
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Leica M series
45
The Fastest Route to Success
Your Leica stereomicroscope has been delivered in completely assembled
condition by your Leica partner, and naturally you want to get right to
work. Therefore, your next step should be to study the Quick Start Guide,
which outlines the most important steps at a glance.
This manual will then familiarize you with the finer details of your micro-
scope. The following pages contain important, practical information that
makes using it every day easier.
Take time to read it – it's worth it!
The M series Quick Start Guide (included with instrument).
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47
Overview of an M series Microscope
Click an entry or a position on the microscope to go directly to the corre-
sponding part of the manual.
1
2
3
4
Magnification changer (zoom)
Switch click stops on/off
Coarse/fine focusing
Fastening screw for the binocular tube
(or accessories)
7
5
6
7
Interchangeable objective
Adjustable eyepiece tubes
Eyepieces for spectacle wearers with dioptric
correction and eyecups
9
8
6
4
2
1
11
8
9
Fastening screws for the eyepieces
Trinocular tube
10
3
10 Built-in iris diaphragm
5
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48
The Correct Interpupillary Distance
The interpupillary distance is correctly set if you
see a single circular image field when looking
at a specimen.
Adjusting the Interpupillary Distance
1. Look into the eyepieces.
3. Slowly approach the eyepieces with your
eyes until you can see the complete image
field without corner cutting.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
If you are still a novice microscope user, you
may need a short time to become accustomed
to this. Not to worry—after a little while, it will
become automatic.
Reference value
The distance between eye and eyepiece
measures approx. 22 mm for 10/23B wide-field
eyepieces for persons wearing glasses.
✓
✗
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Leica M series
49
Using the Eyepieces
The eyepieces form the connection between
the tube and the eye of the observer. Simply
push them into the tube and they are ready to
use.
If you do not wear glasses:
1. Hold the eyepiece firmly and rotate the
eyecups forwards counterclockwise.
If you wear glasses:
1. Hold the eyepiece firmly and rotate the
eyecups counterclockwise towards the rear,
as otherwise the viewing distance is too
great.
2. If an eyepiece is equipped with the inte-
Each eyepiece offers a certain magnification
factor that has a determinative effect on the
total magnification. Furthermore, all Leica
eyepieces can be equipped with practical grati-
cules that enable measuring and quantifying of
specimens.
Dioptric correction
A built-in dioptric correction is available for
eyeglass wearers. For more information, refer
to page 60.
grated dioptric correction, turn the value to
the "0" mark.
2. If an eyepiece is equipped with the inte-
grated dioptric correction, turn the value to
the "0" mark.
By the way, one benefit of viewing with
eyeglasses is a drastically lower risk of bacte-
rial transmission (see page 59). The soft mate-
rial of the eyecup also ensures that your glasses
will not be scratched, even if they contact the
eyepiece.
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Leica M series
50
Focusing
Focusing raises or lowers the stereomicroscope
using the focusing drive. The specimen detail is
brought into sharp focus as soon as it is in the
focal point of the objective.
Focusing
Coarse/fine adjustment
The sharpness is adjusted using the coarse/fine
adjustment.
★
The inner, coarse adjustment is used for
covering great distances.
★
The outer, fine adjustment is used for fine
focusing.
The coarse/fine adjustment carries a
load of up to 15 kg.
★
The focusing drive can be operated either
left- or right-handed.
•
★
The resolution of the coarse/fine adjust-
ment is 1 µm.
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Adjusting the resistance of the focus drive
Adjusting the resistance
Is the focus movement too loose or too tight?
Does the outfit tend to slide downwards? The
resistance can be adjusted individually depend-
ing on the equipment weight and personal
preferences as follows:
1. Grip the outer drive knobs with both hands
and turn them towards each other until the
desired resistance is reached during focus-
ing.
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Leica M series
52
Changing the magnification (zoom)
All M series microscopes have an integrated
zoom. The name indicates the zoom range
covered:
Zooming
1. Look into the eyepieces.
2. Focus on the specimen.
★
★
★
Leica M125 = 16.5:1
Leica M165 C = 16.5:1
Leica M205 C = 20.5:1
3. Rotate the magnification changer until the
desired magnification is configured.
The rotary knob for the zoom can be used either
left or right-handed.
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53
Ratchet Steps and Magnification Levels
The zoom button can optionally be operated
either with or without ratchet steps. Continu-
ous zoom is possible when the ratchet steps are
disabled, which many users find convenient.
On the other hand, when the ratchet steps are
enabled, photographs, measurement results
etc. can be reproduced more accurately.
Enabling and disabling ratchet steps
1. Push the top button downwards to disable
the ratchet steps.
Magnifications and
fields of view
information about the magnifications and field
of view diameters, with consideration given to
the position of the magnification changer and
the eyepiece and objective combination used.
2. Push the bottom button upwards to enable
the ratchet steps.
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54
Parfocality: More Comfort and Convenience for Your Work
All Leica stereomicroscopes are parfocally
matched, meaning that you can view a focused
specimen from the lowest to the highest magni-
fication without having to refocus. There is no
need to readjust the focus to view of specimen
location that is located higher or lower.
Parfocality
1. Enlarge the view to the maximum level.
2. Focus on the specimen.
You are done! Even if you select a smaller work-
ing distance, the specimen remains pin-sharp.
Requirements for parfocal work
★
If you are using an eyepiece with dioptric
correction, the procedure differs from this
description. For more information, refer to
page 61.
The parfocality is maintained until you focus on
another level of the specimen.
★
For the procedure for adjusting the parfo-
cality for the objective revolver, refer to
page 26.
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Leica M series
55
Iris Diaphragm
The iris diaphragm in the optics carrier of your
M series microscope has the same purpose as
those in a camera shutter: it regulates the avail-
able light, which changes the depth of field. The
"depth of field" is the area of a specimen that is
brought into sharp focus.
Closing the iris diaphragm
Opening the Iris Diaphragm
★
Close the iris diaphragm by turning the
knob to the left. The subject appears darker
and the depth of field increases.
★
Open the iris diaphragm by turning the
knob to the right. The subject now appears
brighter, but the depth of field decreases.
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Leica M series
56
Magnification Factors of the Eyepieces
An eyepiece not only makes it possible to look passively into the micro-
scope, but also has a critical effect on the maximum magnification. The
magnification factor is between 10x and 40x.
The following eyepieces are available for the M series:
Magnification
Dioptric correction
5 diopter settings
5 diopter settings
5 diopter settings
5 diopter settings
Order number
10 450 023
10 450 024
10 450 025
10 450 026
10×
16×
25×
40×
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Manual
58
Health Notes
Potential sources of infection
Direct contact with eyepieces is
a
•
potential transmission method for
bacterial and viral infections of the eye. The risk
can be kept to a minimum by using individual
eyepieces or detachable eyecups. Eyecups can
be ordered separately. Please contact your
Leica partner.
Separate eyecups are an effective way of
preventing infections.
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59
Dioptric correction
All Leica eyepieces are also available with
built-in dioptric correction, allowing the micro-
scope to be used without glasses even by those
with vision problems. The correction comprises
5 diopter settings.
Using the Dioptric Correction
1. Set the dioptric correction of both eyepieces
to the mid position ("0" diopter settings.
Note that when using dioptric correction,
the advantage of parfocality is lost—thus
you have to manually refocus each time you
change the zoom level. To also use parfocality
with dioptric correction, refer to the instruc-
2. While wearing your glasses, look through
the eyepieces and focus on the specimen.
3. Rotate both eyepieces to the maximum
value of "+5".
4. Hold one eye closed and rotate the other
eyepiece in "-" direction until the specimen
appears sharp.
5. Then, open the other eye and correct the
diopter settings until the image is uniformly
sharp.
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60
Dioptric Correction and Parfocality
Leicastereomicroscopesareparfocallymatched.
The prerequisite for this is the correct setting of
the diopters and the parfocality. The following
adjustments only have to be carried out once
by each user.
Adjusting
7. Look into the eyepieces.
1. Set the dioptric correction for both
eyepieces to "0".
8. Slowly rotate each eyepiece individually
in the "–" direction until each eye sees the
object sharply imaged.
2. Select the lowest magnification and focus
on a flat specimen.
9. Select the highest magnification and refo-
cus if necessary.
Preparations
3. Select the highest magnification and read-
just the sharpness.
★
★
Move the lever of the video/phototube to
the "observation" position and open the
diaphragm.
Now, if you adjust the magnification from the
lowest to the highest level, the specimen is
always brought into sharp focus. If not, repeat
the process.
4. Select the lowest magnification again, but
do not look into the eyepieces.
If you are using the microscope carrier AX,
set it to stereoscopic observation.
6. Rotate the eyepieces counterclockwise in
the "+" direction as far as they will go (+5
diopter settings).
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Graticules
Use
2. Clamp the graticule on the insert, applying
moderate pressure. Ensure that the grati-
cule fits tightly.
4. You can now align the graticule by rotating
the eyepiece in the tube and then tighten-
ing it using the clamping screw.
Graticules make length measurements and
counting easier, particularly for workstations
that are not equipped with a digital camera and
LAS software.
The graticules for length measurements and
numbering are fitted in mounts and are inserted
into the eyepieces.
1. Screw the insert off of the eyepiece.
3. Screw the insert and graticule firmly into
place and replace the eyepiece in the tube.
Use with the AX carrier
If possible, measure with the micro-
scope carrier AX in vertical position. The
measurements are more accurate without the
convergence angle in the stereoscopic image.
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62
Photography & Video
For most microscope users, digital documen-
tation has become an invaluable part of their
work. Research results can be presented in an
attractive manner; measurements on the digi-
tal image provide clarity and, in conjunction
with the motorized IsoPro™ cross-stage, even
images of large specimens can be captured
step by step and automatically joined to create
a new complete image.
Leica DFC cameras
Leica Application Suite
However, if you require absolute control
over the camera and need the capability for
measurement, evaluation and more in addition
to photography, the digital Leica DFC cameras
are exactly right for you. Together with the
Leica Application Suite, they provide virtually
limitless freedom of use. For additional informa-
tion about Leica cameras, refer to the camera's
documentation.
The "Leica Application Suite", or "LAS" for
short, is, as it were, the digital extension of the
Leica M series microscopes. In addition to
capturing images, it lets you control the micro-
scope, illuminator, stages, cameras and more.
For additional information, refer to the LAS
online help.
Adapter
If camera control using the Leica Application
Suite is not required, conventional mirror reflex
and rangefinder cameras from third-party
manufacturers can be used. For this purpose,
Leica Microsystems offers a variety of adapters
that can be used together with the 50% and
100% trinocular tubes.
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64
Photo tubes and C-mounts
Application
If there is unwanted shading at the corners even
with a compatible C-mount adapter installed, it
can be corrected using the "Shading function"
of the camera software.
Cameras from third-party suppliers
All Leica DFC cameras are equipped with a
standardized C-mount interface. In turn, the
C-mount adapter for the respective trinocular
tube is connected to this interface. This adapter
creates a solid mechanical connection between
the microscope and camera and ensures opti-
mum rendering of the microscopic image on
the image sensor of the camera.
In addition to Leica DFC cameras with the stan-
dardized C-mount interfaces, you can connect
third-party cameras to the microscope using
a T2 bayonet adapter. To do so, instead of the
C-mount adapter, simply use the correspond-
ing SLR adapter with T2 connection. However,
these third-party cameras are not integrated
into the Leica Application Suite and have to
be operated using the corresponding software
from the camera manufacturer.
Alternatively, you can also use a C-mount
adapter with higher magnification. This primar-
ily avoids the critical border area of the field of
vision and concentrates on the center of the
field of vision.
Usually, the ideal is for the digital camera to
capture as much of the field of vision as possi-
ble, while excluding as much of the black edge
of the field of vision as possible. To do so, the
magnification factor of the C-mount adapter
must match the image format of the sensor as
closely as possible (see table).
Camera
optimal
(large
suitable
(smaller
The Leica digital cameras are detailed in a sepa-
rate user manual along with instructions for
their assembly and use.
image field)
image field)
DFC290
DFC420
DFC490
DFC500
0.5×
0.5×
0.63×
0.63×
0.8×
0.63×
0.63×
0.8×
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65
Trinocular video/phototube 50%
Use
With its third beam path, the trinocular video/
phototube 50% enables you to simultaneously
view and photograph a specimen. The available
light is divided as follows:
★
★
50% is available for the two eyepieces.
50% of the light is diverted to the video/
photo beam path.
Assembly
Fasten the "trinocular tube 50%" to the optics
carrier instead of the binocular observation
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66
Trinocular video/phototube 100%
Use
Switchover
With its third beam path, the trinocular video/
phototube 100% enables you to either view or
photograph a specimen. This means that 100%
of the light is available to one or the other beam
path. The other beam path remains opaque or
black.
★
Turn the controller on the right side of the
tube into the horizontal position in order to
guide all available light into the eyepieces.
You can now observe the specimen.
★
Turn the controller on the right side of the
tube into the vertical position in order to
guide all available light into the camera.
You can now photograph the specimen.
Assembly
Fasten the "trinocular tube 100%" to the optics
carrier instead of the binocular observation
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Leica M series
67
The Microscope Carrier AX
Users who intend to do a great deal of photog-
raphy, take measurements or work with polar-
ization should select the microscope carrier AX
for stereoscopic/axial observation. The parallax-
free imaging by means of a perpendicular beam
path leads to results without parallax error.
Stereoscopic viewing
A stereoscopic view is required for examining
spatial objects. For this purpose, the micro-
scope carrier AX must be brought to its home
position.
1. Unscrew the clamping screw.
Compatibility
The planachromatic and planapochromatic
objectives fit into the thread in the microscope
carrier AX for the Leica M125, Leica 165 C and the
Leica M205 C.
2. Push the microscope into the home posi-
tion (mid position) to obtain a spatial view
and tighten the clamping screw.
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The Microscope Carrier AX (continued)
Parallax-free images
The objective is now located directly beneath
The parallax-free imaging produces more
precise results in photography, measuring and
polarization.
either the left or the right beam path.
Coaxial light and the Leica M205 C
When using coaxial incident light together with
the Leica M205 C, unscrew the clamping screw
and press the optics carrier towards the right
(caution: the movement is only 2 mm). This
provides uniform light intensity in both beam
paths.
1. Unscrew the clamping screw.
2. Push the microscope towards the left as
far as it will go and tighten the clamping
screw.
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70
The Objective Nosepiece
Use
Parfocal work
Technical constraints
The objective nosepiece cannot be
used together with the LED5000 MCI™
system illumination, as the objective nosepiece
can no longer be rotated.
The objective nosepiece enables you to switch
between two objectives using just one hand
movement, for example between a 1× and a 2×
planapochromat.
With the new M series objectives, the parfo-
cality is maintained even when objectives are
changed, meaning that the specimen remains
in sharp focus during the change.
•
Older Leica objectives can continue to be
used, but without parfocality during the
objective change.
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71
The Different Types of Objectives
To meet the various requirements regarding
imaging properties, there is a choice of high-
quality interchangeable planachromatic and
planapochromatic objectives and also lower-
priced interchangeable achromatic objectives.
Achromatic objectives
Achromatic objectives with a long focal
length:
For special applications achromatic objectives
with long working distances and focal lengths
of f=100 mm to 400 mm are available.
The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2×
achromatic objectives offer countless variants
for selecting the object field diameter, magni-
fication ranges and working distances (see
page 112).
★
★
★
Achromatic objectives are particularly
suited for specimens with high-contrast
structures.
Planachromatic objective 1×
For the highest requirements for overall image
quality, we recommend equipping the micro-
scope with the 1× plan (flat-field) objective,
which returns sharp, contrast-rich object fields.
Flat-field (planachromatic) objectives are
particularly well suited for studying flat
objects such as wafers and thin sections.
With planapochromatic objectives, the
finest structures are visible with high
contrast. The sophisticated apochromatic
correction allows these objectives to attain
the highest color brilliance and fidelity.
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73
Transmitted-light Base TL ST: Controls
1
2
1
2
3
3
Extension plate of the Transmitted-light Base TL ST
4
Rear side of the transmitted-light base TL ST
1
2
3
Screws for changing the halogen lamp
Power connection socket
Power switch
1
Adapter plate for easy assembly of focusing
drives
2
3
4
Removable glass plate
Controller for light intensity
Adjustment for deflection mirror
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75
Transmitted-light Base TL ST: Operation
Light intensity control
Transmitted-light control
Bright field
The left control adjusts the intensity of the
12V/20W halogen illumination.
The transmitted-light base TL BFDF has a slider
that automatically moves the deflection mirror
in the base when moved. The mirror is kept in
the correct position at all times and permits
smooth changeover between bright field and
opaque transmitted light.
Bright field is suitable for examining translu-
cent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
1. Switch on the illumination of the base at
the power switch.
3. Focus on the specimen.
★
Move the slider backwards until the desired
effect is achieved.
3. Set the illumination to the desired intensity
using the left control.
Inclined transmitted light
Transmitted light that traverses the object
obliquely will produce effects advantageous for
observing semitransparent, opaque objects.
★
Slowly pull the slider towards yourself until
the desired effect is achieved.
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Leica M series
76
Transmitted-light Base TL ST: Lamp Replacement
Changing the Halogen Lamp
Changing bulbs
Precautionary measures
When inserting the lamp, ensure that
the cables are inside the two metal
clamps. This prevents the cables from getting
caught during insertion.
Before you change the lamp, it is abso-
1. Unscrew the two screws on the cooling unit
and pull the cooling unit out, along with
the lamp.
•
lutely necessary to unplug the power
plug from the base to prevent the risk of elec-
tric shock!
•
The halogen lamp becomes very hot
during operation. Therefore, to avoid
being burned, let the base cool off for approx.
10 minutes after switching it off!
Do not touch new halogen lamps with
•
your bare fingers—this drastically
reduces the service life of the lamp!
2. Carefully pull out the lamp and mount by
pulling them upwards.
3. Disconnect the lamp from the mount.
4. Insert the new lamp into the mount and
reinsert the lamp holder.
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77
Transmitted-light Base TL BFDF: Controls
1
2
Adapter at the focusing drive
3
Extension plate of the Transmitted-light Base TL BFDF
1
Adapter plate for easy assembly of focusing
drives
2
3
Standard stage 10 447 269
Button to toggle between bright field and
dark field
Connector for cold light sources
(light conductor active f = 10 mm,
end tube f = 13 mm)
Button to toggle between bright field/ dark field
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Leica M series
78
Transmitted-light Base TL BFDF: Operation
Light intensity control
Please observe the user manual—in
particular, all safety regulations—from
the manufacturers of the light guide and cold
light source.
Bright field
Dark field
Bright field is suitable for examining translu-
cent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
In dark-field illumination, a ring illuminator is
used in such a way that the direct light
does not reach the objective without a speci-
men. Only the structure of semitransparent,
opaque objects disperses the light, making the
object visible against a dark background.
•
★
Switch on the cold light source according to
the manufacturer's user manual and adjust
the brightness.
★
Turn the control as far as it will go towards
"BF" ("bright field").
★
Turn the control as far as it will go towards
"DF" ("dark field").
Transmitted-light control
The TL BFDF transmitted-light base has a poten-
tiometer that switches the light from "bright
field" to "dark field".
Fingertip with bright field illumination
Identical subject with dark field illumination
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Leica M series
79
TL RC™ / TL RCI™: Controls
1
2
1
2
3
4
5
6
7
3 4 5
6
Vertical column - transmitted-light base adapter
plate
1
2
3
4
5
6
Power switch
Power connection socket
USB socket, type B
USB socket, type A
2× CAN bus
1
Heat sink of the integrated halogen illumina-
tion (only TL RCI™)
2
3
4
5
Extension plate for focusing drives
Standard stage 10 447 269
Filter holder
Control of top and bottom flaps of the
Rottermann Contrast™
Screws for changing the halogen lamp
6
7
Button for mirror and horizontal movement
of the mirror
Transmitted-light base
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Leica M series
80
TL RCI™: The Deflection Mirror
Tilted mirror
Functions of the rotary knob
The built-in mirror features one flat and one
concave side and can be rotated and moved.
The concave side has been specially designed
for the optical requirements of objectives with a
high numerical aperture. The black rotary knob
on the left side of the transmitted-light base
can be used to rotate the built-in path-folding
mirror and move it forwards/back.
The rotary knob (1) fulfills the following tasks:
★
★
Turning the deflection mirror from the flat
to the concave side.
Slight tilting to guide the light beam
through the specimen plane at a steeper or
flatter angle
1
The concave cutout on the handle indicates the
concave side of the mirror, allowing intuitive
operation at any time without eye contact.
★
Moving the deflection mirror (forwards/
back)
Inverted world?
Depending on the properties of the specimen
(refractive index of the environment) and the
perception of the viewer, it is possible that
the switches described below for positive and
inverted relief contrast are to be operated in
reverse. This means that the lower switch, rather
than of the upper switch, controls inverted relief
contrast and vice-versa.
The angle of light incidence in the specimen
plane changes depending on the tilt and posi-
tion of the mirror. As a result, switching between
transmitted light bright field, oblique illumina-
tion and dark field-like illumination is possible.
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Leica M series
81
TL RCI™: Color Intensity and Temperature
The transmitted-light base TL RCI™ has two
electronic potentiometers that control the color
intensity (1) and color temperature (2).
Using a USB mouse (only TL RCI™)
The Leica USB mouse controls the CCIC™ and
dimming function of the TL RCI™ base. Connect
the mouse to the corresponding USB port of
the base.
The controller for the color temperature simul-
taneously serves as an electronic shutter:
★
The scroll wheel of the mouse is occupied
by default with the CCIC™ control system
and is used to control the illumination
intensity.
★
★
To interrupt your work, click the potentio-
meter (2).
1
2
Click again to switch on the illumination.
The electronics return to the previously
configured settings.
★
★
To switch the illumination on or off, briefly
click the scroll wheel.
1
2
Potentiometer for controlling the CCIC™
(Constant Color Intensity Control) illumina-
tion intensity
To enter or exit dimming mode, click and
hold down the scroll wheel for longer than
2 seconds.
Potentiometer for controlling the IsoCol™
color temperature
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Leica M series
82
TL RC™ / TL RCI™: Operation
Setting the relief contrast
Depending on the flap position, a part of
the opening of the built-in Fresnel lenses are
covered, which results in the different contrast
effects. Phase structures typically act as spatial,
relief-type images—in the positive relief
contrast like hills, in the inverted relief contrast
like valleys.
Increased contrast without relief is attained
if both diaphragms are set to 45°. A gap-like
illuminated area is created. By tilting the deflec-
tion mirror slightly, you can move the gap over
the entire field of view and quickly toggle
between positive and negative relief images.
The dynamic effect makes it easy to distinguish
phase structures from amplitude structures.
The two switches on the left side of the trans-
mitted-light base TL RC™/TL RCI™ activate two
built-in flaps. The outer switch (1) controls the
inverted relief contrast, and the inner switch (2)
adjusts the positive relief contrast.
1
2
3
1
2
3
Switch for adjusting the inverted relief
contrast
Switch for adjusting the positive relief
contrast
Deflection mirror
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Leica M series
83
TL RCI™: Methods in Transmitted Light
Vertical bright-field illumination
Suitable for stained amplitude specimens with
sufficient contrast.
Inclined transmitted light
Single-sided dark field
Suitable for fixed specimens and fine struc-
tures.
Suitable for semitransparent, opaque speci-
mens such as foraminifera and fish eggs. Move
the deflection mirror until the desired data are
visible.
The light beams are deflected vertically through
the specimen. This results in an accurate bright
field with maximum brightness.
The flatter the angle at which the light beams
are deflected into the specimen plane, the
darker the substrate appears. A dark field-like
transmitted light is created. Outlines, fine edges
and structures are bright, in contrast with the
dark background, through diffraction of the
light beams on the dark background.
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Leica M series
84
TL RCI™: Relief Images
Starting position
Positive relief contrast
Negative relief contrast
Suitable for semitransparent and transparent
specimens. The phase structures look like hills.
Suitable for semitransparent and transparent
specimens. These settings result in a nega-
tive relief contrast. Phase structures look like
valleys.
1. Push the deflection mirror all the way back
towards the column.
The effect can be strengthened or weakened by
gently tilting the deflection mirror.
2. Turn the deflection mirror into the notch
position at an angle of 45°.
The effect can be strengthened or weakened by
gently tilting the deflection mirror.
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Leica M series
85
TL RCI™: Relief Images (continued)
Dynamic relief contrast
Constraints
Suitable for semitransparent and transparent
specimens.
The relief methods provide good results from
mid-zoom to high magnifications and with 1×,
1.6× and 2× magnifications. In the lower zoom
half and with weaker specimens, the object
field may not be uniformly illuminated.
By tilting the deflection mirror slightly, you can
move the gaps over the entire field of view and
quickly toggle between positive and negative
relief images. The dynamic effect makes it easy
to distinguish phase structures from amplitude
structures.
We recommend using the transmitted-light
base with 1× or higher objectives, and not
objectives with a long focal length.
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Leica M series
86
Using Filters
Filters for TL RC™ and TL RCI™
2. Take the empty filter from an available filter
slot in the filter holder.
Daylight filter for TL ST
daylight filter is also available for the
transmitted-light Base TL ST.
The transmitted-light bases TL RC™ and
TL RCI™ can be equipped with up to three
filters—available as accessories—simultane-
ously. By customer request, the filters are also
available as one-off items.
A
3. Insert the desired filter.
1. Switch off the light source or click (TL RCI™)
the button for the shutter.
4. Switch the light source back on.
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Leica M series
87
IsoPro™ (Motorized): Controls
IsoPro
X-Y Stage DCI module
Leica PSC Controller
1
1
2
2
3
1
2
3
4
1
2
3
4
Sub-D interface for Leica SmartMove™
3 CTL2 interfaces
USB interface (type B)
1
2
3
Quick control/memory function
Fine control in X direction
Fine control in Y direction
1
2
Motorized cross-stage Leica IsoPro™
Housing with motorization
Socket for grounded power cable
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Leica M series
89
Leica LED5000 MCI™
Use
Contact with the base
If the optics carriers are accidentally
lowered too far, contact between the
base and MCI may result. A safety mechanism
in the linkage ensures that in this case, the MCI
is automatically pushed upwards to prevent the
possibility of damage.
The Leica LED5000 MCI™ (for "Multi Contrast
Illumination") is a universal high-output illu-
minator. Three groups of 3 LEDs each can be
arranged as desired around the specimen. The
targeted control of the LEDs enables various
illumination scenarios to be implemented.
•
Preparation
Installed too high
★
★
Be sure not to place any specimens directly
beneath the MCI.
Hold the MCI with both hands and pull it down-
wards until the illuminator clicks into place on
the bottom end of the guide rods.
After the optics carrier is lifted, put the MCI
back into the original position.
In this position, you always have the same
contrast with identical illumination. This guar-
antees the reproducibility of an experiment.
Optimum height
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Leica M series
91
Leica LED5000 MCI™ (continued)
Using the keyboard
The illumination scenarios
★
Use the key to switch the illumination on
or off.
Use the " " and " " keys to toggle between the
illumination scenarios shown below.
★
Use the "+" and "–" keys to adjust the
brightness in 10 increments. Touch either
of the two buttons to adjust the intensity in
small increments. Hold a key to change the
intensity more quickly.
Maximum brightness
For temperature reasons its is not possible to
switch on all nine LEDs simultaneously. There-
fore, to provide bright overall illumination, the
top two rows are activated. The bottom row
primarily provides contrast.
The Leica LED5000 MCI™ can also be controlled
by the LAS (Leica Application Suite) software.
Maximum brightness
Maximum contrast
Point illumination
from the rear
Point illumination
from the left
Point illumination
from the right
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Leica M series
92
Leica LED5000 RL
Use
Using the keyboard
The illumination scenarios
The Leica LED5000 RL ("Ring Light") generates
a very bright and homogenous incident light.
It has a diameter of 80 mm and illuminates the
specimen with 48 LEDs that can be switched on
and off completely or in various combinations.
It is controlled using either the integrated or via
the Leica Application Suite (LAS).
★
Use the key to switch the illumination on
or off.
Use the " " and " " keys to toggle between the
illumination scenarios shown below.
★
Use the "+" and "–" keys to adjust the
brightness in 10 increments. Touch either
of the two buttons to adjust the intensity in
small increments. Hold a key to change the
intensity more quickly.
Constraint
The Leica LED5000 RL has been designed for
use with the 1.0× and 0.63× objectives. For the
1.6× and 2.0×, conventional ring illuminators
from the Leica illuminator product range are
available.
LAS enables you to create fully reproducible
illumination scenarios and automatically toggle
between them. For additional information, refer
to the LAS online help.
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Leica M series
93
Leica PSC Controller
Use
Passing over the specimen quickly
Control by LAS
The Leica PSC controller gives you precision
control of the Leica IsoPro™ motorized cross-
stage.
★
Move the joystick in any desired direction
(including diagonally) to quickly control
the cross-stage.
The instructions for controlling the
Leica IsoPro™ using the Leica Application Suite
are provided in the software's help file.
Connection
Connect the Leica PSC controller and the Leica
IsoPro™ motorized cross-stage.
Fine control of the cross-stage
The motorized Leica IsoPro™ cross-stage offers
an accuracy of up to 0.25µm. To move to a posi-
tion with maximum accuracy, use the left and
right knobs on the joystick.
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Leica M series
95
Leica M125 / M165 C (continued)
Leica M125 / M165 C with transmitted-light base TL ST and manual focus
(dimensions in mm)
292
217
154
154
356
340
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Leica M series
98
Leica M125 / M165 C (continued)
Leica M125 / M165 C with transmitted-light stand TL RC™ and manual focus
(dimensions in mm)
292
217
154
154
385
340
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Leica M series
99
Leica M125 / M165 C (continued)
Leica M125 / M165 C with transmitted-light stand TL RC™, manual cross-stage Leica IsoPro™ and manual focus
(dimensions in mm)
max. 370
292
217
154
187
497
max. 370
X = +-75 mm
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Leica M series
100
Leica M205 C (continued)
Leica M205 C with transmitted-light stand TL RCI™ and manual focusing drive
(dimensions in mm)
292
217
154
154
385
340
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Leica M series
Manual
102
Leica M205 C (continued)
Dimensions of Leica M205 C with transmitted-light stand TL RCI™, manual cross-stage Leica IsoPro™ and manual focus
(dimensions in mm)
292
217
497
154
187
max. 410
X = +-75 mm
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Leica M series
103
Transmitted-light Base TL ST
Light source
Halogen lamp 12V/20W
Quick illuminant change
Illuminated area
Power supply unit
Yes
50 mm
Input voltage 100 – 240 V~, frequency 50 – 60 Hz
energy consumption 30 W max.,
ambient temperature 10–40 °C
Power plug, power switch
7.4 kg
Connections
Weight
Illumination types
Bright field
Yes
Dark field
Yes (single-sided)
Oblique light
No
No
Relief Contrast System (RC™)
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control
Integrated filter holder
Coated optics for increasing the color
temperature
No
Yes
Yes
Matching of high num. aperture
Remote control options
AntiShock™ Pads
No
No
Yes
Dimensions (W×H×D)
340×430×85 mm
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Manual
107
Transmitted-light Base TL BFDF
Light source
External via cold light source
Illuminated area
Connections
40 mm
Connection for cold light guide,
active f=10 mm, end tube f=13 mm
5.8 kg
Weight
Illumination types
Bright field
Yes
Yes
No
No
Dark field
Oblique light
Relief Contrast System (RC™)
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control
Integrated filter holder
Coated optics for increasing the color
temperature
Yes*
No
No
Matching of high num. aperture
Remote control options
AntiShock™ Pads
Yes**
Yes***
Yes
Dimensions (W×H×D)
340×390×90 mm
*With cold light source Leica CLS150 LS **Concave mirror *** With external light source
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Leica M series
108
TL RC™ / TL RCI™
Leica TL RC™
Leica TL RCI™
Light source
External via cold light source
Halogen lamp 12V/20W
Yes
Quick illuminant change
Illuminated area
Power supply unit
–
35 mm
–
35 mm
100 – 240 V~, frequency 50 – 60 Hz
energy consumption 30 W max.,
ambient temperature 10–40 °C
1×USB type A, 1×USB type B, 2×CAN-BUS
Connections
Weight
Connection for cold light guide,
active f=10 mm, end tube f=13 mm
6.0 kg
7.2 kg
Illumination types
Bright field/dark field
Yes/yes
Yes/yes
No
Yes/yes
Oblique light/Relief Contrast System (RC™)
CCIC (Constant Color Intensity Control)
Internal shutter/lamp control
Integrated filter holder
Yes/yes
Yes
Yes**
Yes
Yes
Yes
Coated optics for increasing the color temperature
Matching of high num. aperture
Remote control options
Yes
Yes
Yes***
Yes****
Yes
Yes***
Yes
AntiShock™ Pads
Yes
Dimensions of base (W×H×D)
340×390×95 mm
340×440×95 mm
* Single-sided **With cold light source Leica CLS150 LS ***Concave mirror **** With external light source
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Leica M series
109
IsoPro™ Motorized XY Stage
Compatibility
Stage size (L ×W × H)
Travel
Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™)
335.5 mm × 370 mm × 41.5 mm
152 mm × 102 mm (6" × 4")
Speed X,Y
30 mm/s
Motor resolution X,Y
Repeatability X,Y
Positioning accuracy X,Y
Hysteresis X, Y
Motor
0.25 µm
2 µm
20 µm over the entire travel range
30 µm
Direct current (DC) motor with encoder
Slack-free
Backlash
Adapter
All adapters for TL bases
S-shaped acceleration
USB
Acceleration
Computer connection
Control options
Power supply
Leica PSC™, Leica Smart Move, Leica LAS via PC
Input: 100–240VAC 50 / 60 Hz 1.0 A
Output: 15 VDC 2.66 A 40W MAX
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Manual
110
Calculating the Total Magnification and Field of View Diameter
Parameter
Example
MO
ME
z
Magnification of objective
MO
ME
z
1× objective
Magnification of eyepiece
25×/9.5 eyepiece
Magnification changer position
Zoom position 4
q
Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45°
ErgoTube™
q
Coaxial reflected light 1.5×, tube factor
Factor 1.25×
r
r
Factor 1.25x if the planachromatic and planapochromatic
objectives of the MZ125/MZ16 are used on the MS5, MZ6,
MZ75 or MZ95
NFOV Field number of the eyepiece. Field numbers are printed on the
eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.
Calculation example: magnification in binocular tube
Calculation example: field of view diameter in the specimen
MTOT VIS = MO × ME × z × q × r
or
1 × 25 × 4 × 1.5 × 1.25 = 187.5×
N
FOV
∅ OF:
M
O × z ×q ×r
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Leica M series
112
Care, Maintenance, Contact Persons
We hope you enjoy using your high-perfor-
mance microscope. Leica microscopes are
renowned for their robustness and long service
life. Observing the following care and clean-
ing tips will ensure that even after years and
decades, your Leica microscope will continue to
work as well as on the very first day.
Contact address
Care
However, if your instrument should no longer
function properly, contact your technician,
your Leica representative or Leica Microsystems
(Switzerland) AG, CH-9435 Heerbrugg.
★
Protect your microscope from moisture,
fumes and acids and from alkaline, caustic
and corrosive materials and keep chemicals
away from the instruments.
★
Plugs, optical systems and mechanical parts
must not be disassembled or replaced,
unless doing so is specifically permitted
and described in this manual.
E-mail contact:
stereo.service@leica-microsystems.com
Warranty benefits
The guarantee covers all faults in materials
and manufacture. It does not, however, cover
damage resulting from careless or improper
handling.
★
★
Protect your microscope from oil and
grease.
Do not grease guide surfaces or mechani-
cal parts.
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Leica M series
113
Care, Maintenance, Contact Persons (continued)
Protection from dirt
Cleaning polymer components
Permitted measures
Dust and dirt will affect the quality of your
results.
Some components are made of polymer or are
polymer-coated. They are, therefore, pleasant
and convenient to handle. The use of unsuitable
cleaning agents and techniques can damage
polymers,
★
Clean the microscope (or parts of it) using
warm soapy water, then wipe using distilled
water.
★
★
★
Put a dust cover over the instrument when
it will not be used for a long time.
★
For stubborn dirt, you can also use ethanol
(industrial alcohol) or isopropanol. When
doing so, follow the corresponding safety
instructions.
Use dust caps to protect tube openings,
tubes without eyepieces, and eyepieces.
Keep accessories in a dust-free place when
not in use.
★
★
Remove dust with a pneumatic rubber bulb
or with a soft brush.
Clean objectives and eyepieces with special
optic cleaning cloths and with pure alco-
hol.
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Leica M series
114
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