Leica DM750
Manual
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Chapter overview
Safety regulations
The Leica DM750
Ready!
5
17
20
30
43
45
48
51
Set!
Go!
Care of the Microscope
Troubleshooting
Dimensions
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Leica DM750
Manual
3
Contents
Safety regulations
Safety concept
Symbols used in this instruction manual
Important Notes
Intended Purpose of the Microscope
Instructions on use
Health risks and dangers of use
Information for the person responsible for the instrument
Care instructions
Accessories, maintenance and repair
Electrical data and ambient conditions
5
6
7
8
9
Set!
30
31
32
33
34
35
36
38
40
42
Turning on the microscope
Complete Condenser Centering
Using the Condenser
Prepare to View a Specimen Slide
10 Focusing
12 Viewing Tube Adjustment
13 Koehler Configuration
14 Oil Immersion Technique
15 Time Delay Shutoff
16
Go!
43
The Leica DM750
Introduction
17 Ready! Set! Go!
18
44
Care of the Microscope
45
Unpacking
19
General maintenance
46
Ready!
Substage Illumination
Attaching the viewing Tubes
Leica EZ Viewing Tube – integrated eyepieces
Leica Standard Viewing Tube – separated eyepieces
Eyecups
20
21
22
23
25
26
27
28
Troubleshooting
48
Troubleshooting
49
Dimensions
51
Installing Objectives
Installing the Substage Condenser
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Manual
Leica DM750
4
Safety regulations
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Manual
Leica DM750
5
Safety concept
The individual modules of the Leica DM
microscopy series include an interactive
CD-ROM with all relevant user manuals in
several languages. Keep it in a safe place, and
readily accessible to the user. User manuals and
updates are also available for you to download
and print from our web site at
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
This user manual describes the special func-
tions of the individual modules of the Leica
DM microscopy series and contains important
instructions for their operational safety, main-
tenance, and accessories.
The "Safety concept" booklet contains additi-
onal safety information regarding the service
work, requirements and the handling of
microscope, accessories and electrical accesso-
ries as well as general safety instructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
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Manual
Leica DM750
6
Symbols used in this instruction manual
Warning of a danger
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Danger due to hot surface.
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Failure to comply can cause the following:
Ϙ
Hazards to personnel
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Ϙ
Functional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Explanatory notes
ϐ
This symbol within the text points to additi-
onal information and explanations.
Instructions for disposing of the inst-
rument, accessory components, and
Failure to comply can cause the following:
Ϙ
Hazards to personnel
consumables.
Ϙ
Functional disturbances or damaged
instruments
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Leica DM750
Manual
7
Important Notes
Description
User Manual
Accessories from third-party suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
The Leica DM750 microscope meets today's
state of the art of technology. Nevertheless,
hazards may still arise during operation. The
potential risks are described below.
This user manual includes important instruc-
tions related to operating safety, maintenance
and accessories,
Your Leica DM750 microscope comes
with an interactive CD-ROM with all
relevant user manuals. Keep it in a safe place,
and readily accessible to the user. User manu-
als and updates are also available for you
to download and print from our website at
Before installing, operating or using the
instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
Original condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Legal requirements
Contact address
Adhere to general and local regulations rela-
ting to accident prevention and environmental
protection.
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
EC Declaration of Conformity
Electrically operated accessories are construc-
ted based on the state of the art of technology
and are provided with an EC Declaration of
Conformity.
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Manual
Leica DM750
8
Intended Purpose of the Microscope
The Leica DM750 microscope, for which
this instruction manual has been writ-
Caution! The manufacturer assumes
no liability for any noncompliant use
Caution! This (IVD) instrument is not
intended for use in a patient envi-
ten, is intended for biological routine and
research applications. This includes exami-
ning specimens taken from the human body
for the purpose of gaining information about
physiological or pathological conditions
or inborn anomalies, or testing for safety
and compatibility for potential recipients,
or for monitoring therapeutic measures.
or use outside of the specifications from Leica
Microsystems CMS GmbH as well as any risks
that may arise from such use. In such cases, the
Declaration of Conformity shall be invalid.
ronment as defined by DIN VDE 0100-710.
It is also not intended to be combined with
medical devices as defined by EN 60601-1. If a
microscope is electrically connected to a medi-
cal instrument in accordance with EN 60601-1,
the requirements defined in EN 60601-1-1 shall
apply. Not suitable for examining potentially
infectious specimens.
The above-named microscope complies with
the Council Directive 98/79/EEC concerning in
vitro diagnostics.
Caution! The Leica DM750 microscope
is not intended for use with biologically
hazardous materials.
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Manual
Leica DM750
9
Instructions on use
Place of use
Non-intended use
Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The Leica DM750 microscope may be
used only in closed rooms and must be placed
on a solid substrate.
Only use the instruments in closed, dust free
rooms and between +10°C and +40°C. Protect
the devices from oil, chemicals and extreme
humidity. If using the devices outdoors, protect
them from dust and moisture. Never use electri-
cal devices outdoors. Install electrical devices at
least 10 cm from the wall and away from flam-
mable substances.
The Leica DM750 microscope can be used in
clean rooms without any problems.
The devices and accessories described in
this instruction manual have been tested
for safety and potential hazards.
Always position the Leica DM750
microscope so that you can disconnect
it from the power supply at any time. The power
cable must remain accessible at all times,
because it is intended as a power disconnect
device.
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.
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Leica DM750
10
Instructions on use (continued)
The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Disposal
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for following
all applicable safety instructions, laws and
guidelines.
Once the product has reached the end of its
service life, please contact Leica Service or Sales
about disposal.
Please observe and ensure compliance with the
national laws and regulations that implement,
for example, the EC Directive WEEE.
Unauthorized alterations to the instru-
ment or noncompliant use shall void all
rights to any warranty claims.
Like all electronic devices, this inst-
rument, its accessory components
and consumables must never be disposed of
with general household waste. Disposal must
comply with locally applicable laws and regu-
lations.
Transport
If at all possible, use the original packaging for
shipping or transporting individual modules.
In order to prevent damage from vibrations,
disassemble all moving parts that (according
to the user manual) can be assembled and
disassembled by the customer and pack them
separately.
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Manual
Leica DM750
11
Health risks and dangers of use
Health risks
The ergonomic design and construction of the
Leica microscopy series are intended to reduce
the exertion of the user to a minimum.
Dangers during use
Workplaces with microscopes facilitate
and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
Ϙ
The Leica DM750 microscope may only be
connected to a grounded socket.
Danger of infection
Ϙ
The Leica DM750 microscope may not be
operated unless it is in proper functioning
condition.
Direct contact with eyepieces is
a
potential transmission method for
bacterial and viral infections of the eye.
LED Klasse 1 /LED 1 Laser Product
The illumination meets laser class 1 require-
ments in accordance with IEC 60825-1 "Safety
of Laser devices".
The risk can be kept to a minimum by
using personal eyepieces for each indivi-
dual or detachable eyecups.
Ϙ
Ϙ
Ϙ
Optimum workplace layout
Frequent changes of activity
Caution: Hazardous radiation may occur if
controls, adjustment jigs, or procedures other
than those outlined in this User Manual are
used!
Thorough training of the personnel, giving
consideration to ergonomic and organiza-
tional aspects
Class 1
LED class 1
LED Product
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Leica DM750
12
Information for the person responsible for the instrument
Information for the person responsible for
the instrument
Ϙ
Ϙ
Do not use the Leica DM750 microscope
unless it is in perfect condition.
Ϙ
Ϙ
Only original Leica replacement parts may
be used in servicing the product.
Ϙ
Ϙ
Ϙ
Ensure that the Leica DM750 microscope is
used only by qualified personnel.
Inform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry
Division, 9435 Heerbrugg, Switzerland,
immediately of any product defect that
could potentially cause injury or harm.
After service work or technical modifica-
tions, the unit must be reconfigured with
observance to our technical requirements.
Ensure that this user manual is always
available at the Leica DM750 microscope.
Ϙ
If the unit is modified or serviced by
unauthorized persons, is improperly
maintained (as long as maintenance was
not carried out by us), or is handled impro-
perly, Leica will not accept any liability.
Carry out regular inspections to make
certain that the authorized users are
adhering to safety requirements.
Ϙ
If you use accessories made by third-party
manufacturers with the Leica DM750
microscope, be sure that each such manu-
facturer confirms the safety-engineering,
harmless usability of the product and
observe the product's user manual.
Ϙ
Ϙ
When instructing new users, do so
thoroughly and explain the meanings of
the warning signs and messages.
Ϙ
The electric installation in the building
must conform to the national standard, e.g.
current-operated ground leakage protec-
tion (fault-current protection) is suggested.
Assign individual responsibilities for star-
ting, operating and servicing the instru-
ment and monitor the observance of these
responsibilities.
Ϙ
Modifications and maintenance of the Leica
DM750 microscope may only be performed
by professionals expressly authorized by
Leica.
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Manual
Leica DM750
13
Care instructions
General instructions
Cleaning coated parts and plastic parts
Cleaning glass surfaces
Ϙ
Protect the Leica DM750 microscope
against damp, vapors, acids, alkalis, and
corrosive substances. Do not store chemi-
cals in the vicinity.
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
Dust and dirt particles should be removed
with a soft brush or lint-free cotton cloth.
Ϙ
Remove dust using a dry and grease-free
brush made from hair, by blowing with
bellows, or by using a vacuum.
Remove coarse debris with a moistened
disposable cloth.
Ϙ
Ϙ
Remove dirt using a clean cloth that has
been moistened with distilled water.
Ϙ
Protect the Leica DM750 microscope from
oil and grease. Never grease or oil mechani-
cal parts or sliding surfaces.
Clinging dirt can be removed using petro-
leum ether or alcohol
Remove tough dirt using alcohol or petro-
leum ether.
Ϙ
Ϙ
Follow the instructions of the disinfectant
manufacturer.
Acetone, xylene or nitro-containing thin-
ners must NOT be used.
It is advisable to enter a service agreement
with Leica Service.
Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces, and
specimens could be contaminated by abra-
ded particles.
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Manual
Leica DM750
14
Accessories, maintenance and repair
Accessories
Maintenance
Repairs and service work
Only the following accessories may be used
with the Leica DM750 microscope:
Ϙ
The Leica DM750 microscope is basically
maintenance-free. To ensure that it always
operates safely and reliably, we recommend
that you take the precaution of contacting
the responsible service organization.
Ϙ
Ϙ
Ϙ
Only original Leica Microsystems spare
parts may be used.
Ϙ
The Leica accessories described in this user
manual.
Before opening the instruments, switch off
the power and unplug the power cable.
Ϙ
Other accessories, provided that these have
been expressly approved by Leica as being
technically safe in this context.
You can arrange for periodic inspections
or, if appropriate, conclude a mainte-
Avoid contact with powered electrical
circuits, which can lead to injury.
nance contract with them.
Service address
In case of problems, please contact us as
follows:
Ϙ
It is advisable to enter a service agreement
with Leica Service.
Ϙ
For maintenance and repair, only OEM
spare parts may be used.
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Phone +41 71 726 33 28
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Leica DM750
15
Electrical data and ambient conditions
Fuse Replacement
In order to maintain this condition and to ensure safe operation,
the user must follow the instructions and warnings contained in
this instruction manual.
Unplug the instrument before changing any fuses. The Leica
DM750 has two fuses, which are located behind the power cord
receptacle.
Only use the following fuse types: 5×20 mm, 1 A/250V, fast-acting
fuse (# 13RFAG30003)
Environment
Temperature for use
+10 °C … +40 °C
-20 °C … +52 °C
+50 °F to +104 °F
25 mm on 50 mm hard wood
100 g / 6 ms
Storage temperature
Electrical data
Input: 100-240 V, 50/60 Hz, 5 W (3 W LED)
Manipulation shock
Transport shock (unpacked)
Transport shock (packed)
General safety notes
800 mm free fall
5–200 Hz / 1.5 g
500–1060 mbar
20–90%
This instrument of safety class 1 has been built and tested in accordance
with the following safety requirements for electrical equipment for measu-
rement, control, and laboratory use:
Transport vibrations (unpacked)
Air pressure during use and storage:
Humidity during use and storage:
EN 61010-1: 2002-08
EN 61010-2-101: 2008-06
IEC 61010-1: 2010-06
EN 61326-1: 2006-10
EN 61326-2-6: 2006-10
EN 55011: 2007+A2: 2010-05
EN 60825-1: 2008-05
IEC 60825-1: 2007-03
LED Class 1
Installation Category II (Overvoltage Category)
Pollution degree 2
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Leica DM750
16
The Leica DM750
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Leica DM750
17
Introduction
Thank you for purchasing the Leica DM750
Compound Microscope from Leica Microsys-
tems. This model’s exclusive design features
and full range of accessories make it a truly
versatile, high quality-instrument for educatio-
nal and routine clinical applications.
The instrument is to be used only as
described. Hazards to personnel may
result if used improperly.
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Manual
Leica DM750
18
Unpacking
Ϙ
Carefully remove the microscope and any
components from the packing carton.
Ϙ
Ϙ
Verify that all components are intact.
Check the components against the planned
configuration.
Ϙ
Optional items such as contrast accessories,
camera adapters, cameras, and carrying
cases are not shipped as part of the stan-
dard equipment. These items are delivered
in separate packages.
Ϙ
Please do not discard any of the packing
materials. They should be used for safely
storing and transporting the instrument
should the need arise.
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Leica DM750
19
Ready!
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Leica DM750
20
Substage Illumination
Substage Illumination
Type 1: Standard illumination
Adjustable condenser centering with provided
tool.
Type 2: Koehler illumination
Adjustable condenser centering
thumbscrews and adjustable Koehler field
diaphragm.
The Leica DM750 is available with two
types of Substage Illumination. Identify
which type of Illumination you have, as this will
be important to know later.
with
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Leica DM750
21
Attaching the viewing Tubes
Tools used
There are two types of viewing tube.
Identify which viewing tube you have:
1. Loosen the setscrew (on top of the stand)
using the Allen key provided.
Ϙ
Allen key
2. Set the dovetail into the tripod mount and
tighten the setscrew carefully (without
using excessive force). This precisely posi-
tions the viewing tube onto the optical axis
of the microscope, regardless of the selec-
ted rotation.
Type 1: Leica EZ Viewing Tube with integrated
eyepieces.
Type 2: Standard viewing tube with separate
eyepieces
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Leica DM750
22
Leica EZ Viewing Tube – integrated eyepieces
1. In order to use the captive wingscrew,
2. Reinstall the Leica EZ Viewing Tube onto
the stand.
remove the setscrew that was delivered
with the stand.
Leica EZ viewing tube with integrated eyepieces.
To rotate the Leica EZ viewing tube,
either loosen the set screw on the stand
or replace the set screw with the (optional)
captive thumbscrew.
3. Tighten the captive wingscrew using the
wrench included in the delivery package.
Make sure that the wingscrew part
of the captive wingscrew has been
completely separated before you use the stand:
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Leica DM750
23
Leica EZ Viewing Tube – integrated eyepieces (cont.)
You can now safely rotate the Leica EZ
viewing tube by loosening the captive
thumbscrew, rotating the viewing tube, and
tightening the captive thumbscrew again.
The eyepieces are integrated into the
Leica EZ Viewing Tube and preset; there-
fore, there is no need to adjust or install the
eyepieces.
26.
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Leica DM750
24
Leica Standard Viewing Tube – separated eyepieces
1. Insert the eyepieces into the tubes.
2. Secure the eyepieces in the tubes by tighte-
ning the silver screws at the bottom of the
tubes using a standard Phillips screwdriver
(not included in the delivery package).
Standard viewing tubes; tubes do not include
eyepieces yet
The standard viewing tube has a rota-
table dovetail. Therefore, you can now
rotate the standard viewing tube freely in any
orientation.
The eyepieces will still rotate, but they
will be captive in the eyetubes.
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Leica DM750
25
Eyecups
If you wear eyeglasses for microscope
If you have purchased
a
standard
viewing, keep the rubber eyecups folded
down. If you do not wear eyeglasses, you may
find it useful to unfold the rubber eyecups in
order to help block out ambient room light.
microscope configuration, you will
notice that the objectives are already installed
on the nosepiece and the substage condenser
is already installed on the stand. In this case,
purchased your Leica DM750 in the form of
individual components rather than the stan-
dard configuration, please continue with the
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Leica DM750
26
Installing Objectives
Installing Objectives
When rotating the objective nosepiece,
always use the knurled ring on the
objective nosepiece.
As you rotate the nosepiece clockwise, attach the
objectives by screwing them into the nosepiece
holes starting with the lowest magnification and
advancing to the highest magnification.
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Leica DM750
27
Installing the Substage Condenser
Substage Condenser
The Leica DM750 has an open substage
condenser mount, therefore the conden-
ser needs to be installed.
1. Move the specimen stage upwards as far as
3. Unscrew the two setscrews (or wings-
crews for Koehler stands) on the condenser
holder.
it will go by using the coarse focusing knob
on the side of the microscope.
2. Move the condenser holder into the lowest
position by using the condenser focusing
knob on the left side of the stage mount.
The substage condenser mount is open
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Leica DM750
28
Installing the Substage Condenser (cont.)
4. Push the substage condenser under the
specimen stage into the holder by alig-
ning the locating pin on the bottom of the
condenser with the slot on the rear side of
the fork.
5. Move the condenser into the highest posi-
tion by using the condenser focusing knob
on the left side of the stage mount.
6. Tighten the two setscrews using the tool
included in the delivery package (or, for a
Koehler stand, tighten the two wingscrews)
so that the upper lens of the condenser is
centered under the objective in working
position and the substage condenser is
thus roughly centered.
You will center the condenser more accu-
rately when you get to the "Complete
Condenser Centering" section on page 32.
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Leica DM750
29
Set!
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Leica DM750
30
Turning on the microscope
Work Surface
Setting the Illumination Intensity
Set the illumination to the lowest
setting to start with, using the control-
ler on the bottom left of the stand. The illumi-
nation control knob allows you to adjust the
intensity of light produced by the illumination
system.
Plug in and Turn on the Microscope
1. Plug the power cable of the microscope
into a corresponding grounded socket. A
grounded 3-wire cord is provided.
Always use your microscope on a hard,
stable surface.
2. Switch on the microscope using the switch
at the bottom right of the microscope
stand.
Power Cord
If the power cord is not already atta-
ched, attach it securely to the back of
the microscope.
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Leica DM750
31
Complete Condenser Centering
If you have purchased a standard Leica
DM750 configuration, the condenser has
already been pre-centered by Leica Microsys-
tems.
2. Write an "X" on a piece of paper in the size
of a business card and place it onto the
light output of the microscope stand in
such a way that the "X" is centered over the
illumination.
1. Open the condenser aperture by rotating
the knurled ring on the condenser to the
right.
Not centered
3. Look at the X through the eyepieces and
center it in the field of view by tightening
the setscrews with the tool included in the
delivery package (or, for a Leica DM750
with Koehler illumination, tighten the
knurled screws).
Centered
Make sure that the condenser is in the
highest position.
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Leica DM750
32
Using the Condenser
Using the Condenser
1. To open and close this diaphragm, simply
turn the knurled ring on the condenser to
the right or left so that the line on the ring
is aligned with the objective magnification
used.
2. Open the iris diaphragm of the condenser
completely at first by turning the conden-
ser ring all the way to the right.
The condenser is furnished with an iris
diaphragm, which can be adjusted to
match the effective numerical aperture of the
objective
Match the line of the rotating ring with the
objective magnification in use.
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Leica DM750
33
Prepare to View a Specimen Slide
1. Position a specimen slide on the specimen
stage by sliding it under the slide grips.
2. Using the X/Y-stage control, position the
specimen slide such that a part of the speci-
men is under the objective used.
Slide grips hold the slide in place.
Slide grips
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Leica DM750
34
Focusing
1. Rotate the objective nosepiece (using the
knurled ring) in such a way that the objec-
tive with the lowest magnification level is
rotated into the working position.
2. Move the specimen stage upwards by
turning the coarse focusing knob as far as
it will go to the maximum position.
3. Look into the eyepieces and adjust the illu-
mination intensity to a level that is comfor-
table for your eyes.
The stand of the Leica DM750 has been
calibrated at the factory so that the focus
can be found from this position within 1.5 rota-
tions of the fine focus.
4. Bring the specimen into focus using the
fine focusing knob.
Coarse focus adjustment knob
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Leica DM750
35
Viewing Tube Adjustment
Adjust the eyetubes
Leica EZ Viewing Tube
Ϙ
If you have a Leica DM750 with stan-
dard illumination (without Koehler field
diaphragm), continue with the "Oil Immer-
sion Technique" section on page 40.
1. Adjust the tubes to your interpupillary
distance. Fold or unfold the eyetubes to
decrease or increase the distance between
the eyepieces until you see one illuminated
circle.
Ϙ
If you are using a Leica EZ viewing tube,
which has the eyepieces integrated with
the eyetubes, no additional adjustments
are necessary. Be sure you are wearing your
eyeglasses or contact lenses.
Ϙ
If you have a Leica DM750 with Koehler
field diaphragm, continue with the "Koeh-
ler Configuration" section on page 38.
Ϙ
If you have a Leica DM750 with stan-
dard illumination (without Koehler field
diaphragm), continue with the "Oil Immer-
sion Technique" section on page 40.
Standard Viewing Tube with One or Two
Focusing Eyepieces
Ϙ
If you have a Leica DM750 with Koehler
field diaphragm, continue with the "Koeh-
ler Configuration" section on page 38.
If you are using a standard viewing tube with
one or two focusing eyepieces, you need to
make some adjustments.
1. Set the focusing eyepieces to "0".
The viewing tubes maintain a constant
tube length for all interpupillary settings.
This means that a change of interpupillary
distance does not affect parfocality, magnifica-
tion, or calibrations that depend on magnifica-
tion.
Standard Viewing Tube with Two Fixed
Eyepieces
Ϙ
If you are using a standard viewing tube
with two fixed eyepieces (no focusing
eyepieces), no additional adjustments
necessary. Be sure you are wearing your
eyeglasses or contact lenses.
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Leica DM750
36
Viewing Tube Adjustment (cont.)
If you are comfortable wearing your
corrective lenses (contact lenses or
eyeglasses) for microscope viewing, leave them
on and your adjustments will be minimal.
vision differences between your right eye
and left eye.
5. Now, switch to an objective with a high
magnification level (no oil objective) and
bring the microscope into focus while
looking through the eyepieces with both
eyes.
2. Using the fine focus adjusting knob, focus
on the specimen while looking through
only one of the eyepieces (when using one
eyepiece that is focusable and one that is
not focusable, look through the one that is
not focusable). To help focus, cover or close
The higher magnifications have a shallo-
wer depth of field. Therefore, after focus-
ing with a high magnification, you will find that
when you change to lower magnifications, you
only have to adjust the fine focus slightly, if at
all.
3. Now look with the other eye just through
the other eye.
the other eyepiece (focusing eyepiece). This
time, focus the specimen by using the focu-
sing capability in the focusing eyepiece.
When doing so, do not change the
height of the specimen stage.
Ϙ
If you use a DM750 with standard illumi-
nation, continue with the "Oil Immersion
Technique" section on page 40.
4. Grip the knurled ring on the focusing
eyepiece with one hand and rotate the top
of the eyepiece with the other hand until
the specimen is in focus for this eye and
this focusing eyepiece. This corrects for any
Ϙ
If your DM750 has a Koehler configuration,
please continue with the next section,
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Leica DM750
37
Koehler Configuration
If your Leica DM750 is fitted with a field diaphragm for Koehler Illu-
mination, use the following procedure to assure condenser cente-
ring and focus.
2. Focus the leaves of the field diaphragm using the condenser focusing
knob on the left side of the stage mount.
1. Connect the Koehler field diaphragm to the base of the microscope
so that the diaphragm leaves are in the field of view when you look
through the eyepieces.
Condenser focusing knob
Leaves are in sharp focus
Close the Koehler field diaphragm
Close the Koehler field diaphragm
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Leica DM750
38
Koehler Configuration (cont.)
3. Turn the condenser centering wingscrews simultaneously to center
the image of the field diaphragm.
4. Open the field diaphragm until the diaphragm leaves are just outside
of the field of view.
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Leica DM750
39
Oil Immersion Technique
1. Search for the area on the specimen slide
that you want to study.
3. Add a drop of immersion oil from Leica
to the area of the specimen slide you are
studying.
4. Rotate the oil immersion objective (the
objective labeled "OIL") to the working
position.
2. Move the specimen stage down to the
lowest position using the coarse adjust-
ment knob.
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Leica DM750
40
Oil Immersion Technique (cont.)
5. Slowly move the specimen stage upwards
using the coarse adjustment knob until
the oil drop on the slide comes into direct
contact with the lens of the oil immersion
objective.
7. Look through the microscope and slowly
rotate the fine adjustment knob, so that the
specimen stage moves upwards until the
specimen is in focus.
8. If you are finished working with the oil
immersion objective, clean the front side
of the objective, the specimen slide, and all
other surfaces that have come into contact
with the oil, following the instructions in
page 46.
6. Hold the knurled ring on the objective
turret and swing the objective back and
forth to eliminate air bubbles. Then bring
the oil objective into the final position, so
that the oil drop is between the front lens
of the objective and the specimen slide.
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Manual
Leica DM750
41
Time Delay Shutoff
The Leica DM750 is equipped with a time
delay shutoff capability, which automati-
cally turns the illumination off after 2 hours of
no changes in the intensity control.
Changing the status of the time delay
shutoff
Ϙ
Ϙ
The LED Illumination will flash to indicate
the time delay shutoff status was changed.
1. Rotate the intensity regulator to the lowest
level.
The LED Illumination will flash two times
slowly then stay on when you disable the
Time Delay Shutoff.
Ϙ
All four-position nosepiece stands have
the time delay shutoff enabled as a default
(mostly educational applications).
2. Switch on the instrument.
3. Rotate the intensity regulator to the high-
est level and then back to the lowest level
within five seconds.
Ϙ
The LED Illumination will flash three times
quickly then stay on when you enable the
Time Delay Shutoff.
Ϙ
All five-position nosepiece stands have the
time delay shutoff disabled as a default
(mostly clinical applications).
When you turn the power off and then
turn the power on again, the system will
be in the last Time Delay Shutoff status (Enabled
or Disabled) and you will not see any blinking.
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Leica DM750
42
Go!
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Leica DM750
43
Ready! Set! Go!
Now all you need to do is change objectives, set
the condenser aperture (and field diaphragm if
you have a DM750 Koehler Stand) appropriately
for the objective magnification you are using,
and enjoy the view!
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Manual
Leica DM750
44
Care of the Microscope
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Leica DM750
Manual
45
General maintenance
Storage and handling
Ϙ
The cord wrap allows you to wrap the cord
in such a way that only the length you need
is extended.
Instructions for cleaning
Always use both hands to carry the
microscope. There is a handle on the
back of the microscope and an undercut in the
front for this purpose.
Ϙ
If any optical surface becomes coated with
dust or dirt, clean the surface by blowing it
off with a syringe or brushing it off with a
camel hair brush before attempting to wipe
the surface clean.
Ϙ
Optical surfaces should be cleaned using
a lint-free cloth, lens cloth or cotton swab
moistened with a commercially available
glass cleaner.
Ϙ
It is very important to avoid the excessive
use of solvents, so use them sparingly. The
lint-free cloth, lens tissue or cotton swab
should be moistened with solvent, but
not be wet enough for the solvent to seep
around the lens.
Ϙ
Ϙ
Keep all optical components clean. Clean-
liness is important for maintaining good
optical performance.
The microscope should always be covered
with the plastic dust cover (provided with
the instrument) when it is not in use.
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Leica DM750
46
General maintenance (cont.)
Ϙ
No part of the microscope is quite so vulne-
rable to collecting dirt, dust, and oil as the
front lens of the objective. Whenever you
encounter lack of contrast, cloudiness or
poor definition, carefully check the condi-
tion of the front lens with a magnifier.
Make sure that the cotton tip contacts the
concave lens surface. Check the objective
with a magnifier after cleaning.
Illumination
The Leica DM750 uses LED illumination.
Ϙ
Therefore, no lamp changing is required for
the life of the microscope.
Ϙ
If you need to remove the microscope’s
viewing body, be careful not to accidentally
touch the outer lens surface (located on
the underside of the body). Fingerprints on
this surface will reduce image clarity. This
lens can be cleaned in the same manner as
objectives and eyepieces.
Ϙ
The lower magnification objectives have
fairly large front lenses and can be cleaned
with a cloth or lens tissue wrapped around
a finger and moistened with methanol.
Ϙ
Cleaning 40× and 100× objectives requires
more care. Note: To attain the typical flat-
ness for objectives with higher magnifica-
tion, the objective has a small concave front
lens with a very small radius and slight
curvature. The surface of this front lens can
be readily cleaned with a toothpick cove-
red with a cotton tip, or with a small cotton
swab. Soak the cotton with isopropanol
and squeeze it out until it is almost dry
again. Wipe the front lens lightly without
applying undue force or scrubbing action.
Mechanical Maintenance
Ϙ
Use the dust cover. It is the most impor-
tant way to keep the microscope in good
mechanical and physical condition.
Ϙ
The Leica DM750 has a durable finish. All
non-optical parts of the instrument can
be cleaned with isopropanol or soap and
water. Avoid the use of all other organic
solvents when cleaning the instrument.
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Manual
Leica DM750
47
Troubleshooting
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Manual
Leica DM750
48
Troubleshooting
Stand
Make sure that the socket has voltage.
Check the cable connections.
The microscope does not respond.
Make sure that the stand is connected to the power supply.
Focus
Use the correct immersion medium.
Lay the specimen with the cover glass toward the top.
Make sure that the cover slip thickness is correct and that it meets the specifica-
tions on the objective.
The specimen cannot be brought into focus.
Dark field
Make sure that a DF objective is being used.
The objective aperture is too high (maximum 0.75/ 1.10); if necessary, reduce
the objective aperture through the iris diaphragm on the objective.
Check the condenser centering.
No definite DF contrast is possible.
Open the aperture diaphragm completely.
The image is not uniformly illuminated.
Unwanted stray light.
The objective magnification is too weak. Use a higher magnification.
Clean the specimen and neighboring lens surfaces.
Polarization
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Manual
Leica DM750
49
Troubleshooting (continued)
Bring the polarizer and analyzer into cross position until they reach maximum
darkness (without specimen).
Polarization contrast cannot be adjusted.
Phase contrast
The specimen is too thick, too thin, or too brightly stained.
Refractive indexes of the mounting medium and specimen are identical, so that
there is no phase jump.
The cover glass is not placed uniformly.
Phase contrast cannot be adjusted.
Check that the correct light ring is positioned.
Check the centering of the light rings.
Check the condenser centering.
Open the aperture diaphragm completely.
Specimen Stage
Move the mechanical stage with coaxial drive all the way to the left.
Manually press the screw that holds the mechanical stage even farther to the
left, as far as it will go. Then move the mechanical stage with coaxial drive all the
way to the right.
Positioning range of the stage in the x-direc-
tion decreases after working a long time.
Manually press the screw that holds the mechanical stage even farther to the
right, as far as it will go.
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Leica DM750
50
Dimensions
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Leica DM750
51
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Leica DM750
52
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Leica DM750
53
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Manual
Leica DM750
54
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