Leica Life Jacket NCL L CD7 580 User Manual

NovocastraLiquid  
Mouse Monoclonal Antibody  
CD7  
Product Code: NCL-L-CD7-580  
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.  
Intended Use  
Specificity  
Clone  
Human CD7 antigen.  
LP15  
IgG2b  
Ig Class  
Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain of  
the human CD7 molecule.  
Antigen Used for  
Immunizations  
Mouse myeloma (p3-NS1-Ag4-1).  
Hybridoma Partner  
Liquid tissue culture supernatant containing sodium azide.  
Volume as indicated on vial label.  
Preparation  
Not tested.  
Yes  
Effective on Frozen Tissue  
Effective on Paraffin Wax  
Embedded Tissue  
Immunohistochemistry on paraffin sections.  
Recommendations on Use  
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra  
Epitope Retrieval Solution pH 6.  
Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should  
determine their own optimal working dilutions.  
Visualization: Please follow the instructions for use in the NovolinkPolymer Detection Systems.  
For further product information or support, contact your local distributor or regional office of Leica  
The performance of this antibody should be validated when utilized with other manual staining  
systems or automated platforms.  
Western Blotting: Not recommended.  
Immunohistochemistry: Tonsil.  
Positive Controls  
Membrane  
Staining Pattern  
Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after  
expiration date indicated on the vial label. Storage conditions other than those specified above  
must be verified by the user.  
Storage and Stability  
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,  
reasonable care should be taken when handling it.  
Warnings and Precautions  
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or  
Leica Biosystems Newcastle Ltd  
Balliol Business Park West  
Benton Lane  
Newcastle Upon Tyne NE12 8EW  
United Kingdom  
(
+44 191 215 4242  
CD7-580-L 10/06/2013  
Download from Www.Somanuals.com. All Manuals Search And Download.  
© Leica Biosystems Newcastle Ltd. NCL-L Rev D  

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