Novocastra™ Lyophilized
Mouse Monoclonal Antibody
Topoisomerase II Alpha
Product Code: NCL-TOPOIIA
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Intended Use
Specificity
Clone
Human topoisomerase II alpha.
3F6
IgG1
Ig Class
Prokaryotic recombinant protein corresponding to the C-terminal region of the topoisomerase II
alpha molecule.
Antigen Used for
Immunizations
Mouse myeloma (p3-NS1-Ag4-1).
Hybridoma Partner
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Preparation
Yes.Optimum fixation, Zamboni’s, 10 minutes at 25 °C ( See Stefanini et al.. 1967).
Effective on Frozen Tissue
Yes.
Effective on Paraffin Wax
Embedded Tissue
Immunohistochemistry on paraffin sections.
Recommendations on Use
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH 6.
Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional office of Leica
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Immunohistochemistry: Tonsil.
Western Blotting: RAJI cell line.
Positive Controls
Nuclear.
Staining Pattern
Store unopened lyophilized antibody at 2-8 °C. Under these conditions, there is no significant
loss in product performance up to the expiry date indicated on the vial label. The reconstituted
antibody is stable for at least two months when stored at 2-8 °C. For long term storage, it is
recommended that aliquots of the antibody are frozen at -20 °C (frost-free freezers are not
recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on
the day of use.
Storage and Stability
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it. This reagent contains sodium azide. A
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Warnings and Precautions
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
TOPOIIA 27/07/2012
© Leica Biosystems Newcastle Ltd • NCL- Rev C
(
+44 191 215 4242
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