Leica Dollhouse NCL TOPOIIA User Manual

NovocastraLyophilized  
Mouse Monoclonal Antibody  
Topoisomerase II Alpha  
Product Code: NCL-TOPOIIA  
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.  
Intended Use  
Specificity  
Clone  
Human topoisomerase II alpha.  
3F6  
IgG1  
Ig Class  
Prokaryotic recombinant protein corresponding to the C-terminal region of the topoisomerase II  
alpha molecule.  
Antigen Used for  
Immunizations  
Mouse myeloma (p3-NS1-Ag4-1).  
Hybridoma Partner  
Lyophilized tissue culture supernatant containing sodium azide.  
Reconstitute with the volume of sterile distilled water indicated on the vial label.  
Preparation  
Yes.Optimum fixation, Zamboni’s, 10 minutes at 25 °C ( See Stefanini et al.. 1967).  
Effective on Frozen Tissue  
Yes.  
Effective on Paraffin Wax  
Embedded Tissue  
Immunohistochemistry on paraffin sections.  
Recommendations on Use  
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra  
Epitope Retrieval Solution pH 6.  
Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should  
determine their own optimal working dilutions.  
Visualization: Please follow the instructions for use in the NovolinkPolymer Detection Systems.  
For further product information or support, contact your local distributor or regional office of Leica  
The performance of this antibody should be validated when utilized with other manual staining  
systems or automated platforms.  
Immunohistochemistry: Tonsil.  
Western Blotting: RAJI cell line.  
Positive Controls  
Nuclear.  
Staining Pattern  
Store unopened lyophilized antibody at 2-8 °C. Under these conditions, there is no significant  
loss in product performance up to the expiry date indicated on the vial label. The reconstituted  
antibody is stable for at least two months when stored at 2-8 °C. For long term storage, it is  
recommended that aliquots of the antibody are frozen at -20 °C (frost-free freezers are not  
recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on  
the day of use.  
Storage and Stability  
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,  
reasonable care should be taken when handling it. This reagent contains sodium azide. A  
com  
Warnings and Precautions  
Leica Biosystems Newcastle Ltd  
Balliol Business Park West  
Benton Lane  
Newcastle Upon Tyne NE12 8EW  
United Kingdom  
TOPOIIA 27/07/2012  
© Leica Biosystems Newcastle Ltd • NCL- Rev C  
(
+44 191 215 4242  
Download from Www.Somanuals.com. All Manuals Search And Download.  

Lego Baby Toy ROBOLAB 29 User Manual
Lenmar Enterprises Battery Charger KIT2CR2 User Manual
LG Electronics Cell Phone U400 User Manual
LG Electronics Projector BX327 User Manual
LG Electronics Range LRE3091S User Manual
LG Electronics Washer WM2233HU User Manual
Liebert Network Card LDM User Manual
Marley Engineered Products Electric Heater 402A User Manual
Maytag Clothes Dryer MEDZ400TQ1 User Manual
Metra Electronics Automobile Accessories 99 8710 User Manual